植物乳杆菌YS-1对恶唑酮诱导BALB/c小鼠结肠炎的预防作用

孙鹏; 易若琨; 赵欣

doi:10.13386/j.issn1002-0306.2017.16.058

植物乳杆菌YS-1对恶唑酮诱导BALB/c小鼠结肠炎的预防作用

孙鹏^1,2,3,4,
易若琨^1,2,3,4,
赵欣^1,2,3,4

1. 重庆第二师范学院重庆市功能性食品协同创新中心
2. 重庆第二师范学院重庆市功能性食品工程技术研究中心
3. 重庆第二师范学院功能性食品研发重庆市工程实验室
4. 重庆第二师范学院生物与化学工程学院

基金项目:

重庆高校创新团队建设计划资助项目(CXTD201601040)；重庆市基础科学与前沿技术研究项目(cstc2016jcyjA 0820)；重庆第二师范学院校级科研项目(KY2015TBZC)；

详细信息

作者简介:
孙鹏 (1983-) , 男, 硕士, 讲师, 研究方向:食品营养和功能性食品, E-mail:598535244@qq.com。;

赵欣 (1981-) , 男, 博士, 教授, 研究方向:食品营养和功能性食品, E-mail:zhaoxin@cque.edu.cn。;

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- 收稿日期: 2017-02-06

Preventive effects of Lactobacillus plantarum YS-1 on oxazolone-induced BALB/c mice colitis

SUN Peng^1,2,3,4,
YI Ruo-kun^1,2,3,4,
ZHAO Xin^1,2,3,4

Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education
Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing University of Education

摘要

摘要: 利用动物实验,就植物乳杆菌YS-1（LP-YS1）对结肠炎的预防效果进行研究。实验将小鼠分为五组,分别为正常组、模型组、LB（保加利亚乳杆菌）处理组、低、高浓度LP-YS1（植物乳杆菌YS-1）处理组,分别涂抹（0.20 mL）和灌肠（0.15 mL）1%恶唑酮溶液诱导BALB/c小鼠结肠炎。分别检测各组小鼠疾病活动指数（DAI）值、结肠重量/结肠长度比值、血清细胞因子水平（IL-2和IL-10）和结肠组织抗氧化相关指标（MPO、NO、MDA、GSH）和n NOS、e NOS、i NOS、c-Kit、SCF、IL-8、CXCR2等的mRNA表达。实验结果显示,LP-YS1显著降低（p<0.05）结肠炎小鼠的DAI,并能抑制结肠炎造成的结肠长度缩短,提高结肠重量/结肠长度比值,还可显著降低（p<0.05）结肠炎小鼠结肠组织MPO、NO、MDA活性和提高GSH活性,并显著提高（p<0.05）结肠炎小鼠血清中的IL-2水平和降低IL-10细胞因子水平。RT-PCR结果表明,LP-YS1可显著上调（p<0.05）结肠炎小鼠结肠组织中的n NOS、e NOS、c-Kit、SCF表达和下调i NOS、IL-8、CXCR2表达。LP-YS1结肠炎具有良好的预防效果,且高浓度的LP-YS1具有更好的预防效果。
- 植物乳杆菌 /
- 恶唑酮 /
- 结肠炎 /
- BALB/c小鼠 /
- mRNA表达
Abstract: In this study, the colitis preventive effects of Lactobacillus plantarum YS-1 (LP-YS1) were studied using an animal experiment.The mice were divided into five groups, which were normal group, model group, LB (Lactobacillus bulgaricus) treated group, low and high concentrations of LP-YS1 (Lactobacillus plantarum YS-1) treated groups, the research object smeard (0.20mL) and clystered (0.15 mL) 1%oxazolone induced colitis in BALB/c mice were detected respectively.The mice DAI value, colon weight/length ratio, serum (IL-2 and IL-10 cytokine levels) and colon tissue index (MPO, NO, MDA, GSH levels and n NOS, e NOS, i NOS, c-Kit, SCF, IL-8, CXCR2 mRNA expression) were used for determination of preventive effects of LP-YS1.The experiment results showed that LP-YS1 significantly reduced (p<0.05) DAI of colitis mice.It could also inhibit the colon length shortening caused by colitis and increase the weight/length ratio of colon.LP-YS1 could significantly reduce the activity of MPO, NO, MDA and increase the activity of GSH in colon tissue of mice with colitis (p<0.05) .LP-YS1 also could significantly increase the serum IL-2 cytokine level and reduce IL-10 level in mice with colitis (p<0.05) .RT-PCR results also showed that LP-YS1 could significantly increase (p<0.05) n NOS, e NOS, c-Kit, SCF expression and decrease i NOS, IL-8, CXCR2 expression in colon tissue of mice.Thus, it can be seen that LP-YS1 has a good preventive effect on oxazolone induced experimental colitis.
- Lactobacillus plantarum /
- oxazolone /
- colitis /
- BALB/c mouse /
- mRNA expression

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参考文献(18)

[1]	樊慧丽, 陈玉梅.溃疡性结肠炎的发病机制和治疗进展[J].中国全科医学, 2012, 15 (1B) :228-230.
[2]	孙芳美.溃疡性结肠炎的发病机制与治疗进展[J].中国医药指南, 2012, 10 (12) :445-447.
[3]	韦宜宾, 刘锟荣, 陈国忠.中药治疗溃疡性结肠炎研究进展[J].辽宁中医药大学学报, 2015, 17 (3) :222-224.
[4]	崔海宏, 陈村龙, 孙勇, 等.溃疡性结肠炎患者肠黏膜菌群改变及抗体反应[J].中国微生态学杂志, 2003, 15 (3) :151-155.
[5]	孙勇, 丁彦青.溃疡性结肠炎患者肠道菌群与病理变化关系的探讨[J].现代消化及介入诊疗, 2009, 14 (1) :26-28.
[6]	吴春生, 舒畅, 李键, 等.牦牛酸乳中乳酸菌的研究进展及前景展望[J].食品工业, 2012 (9) :135-139.
[7]	吴春生, 李键, 骞宇, 等.牦牛乳及牦牛酸乳营养价值的研究现状[J].乳业科学与技术, 2012, 35 (3) :43-46.
[8]	周小芳, 易若琨, 赵欣.Lactobacillus fermentum Zhao的小鼠抗皮肤老化效果[J].重庆第二师范学院学报, 2016, 29 (5) :160-167, 170.
[9]	Qian Y, Suo HY, Du MY, et al.Preventive effect of Lactobacillus fermentum Lee on activated carbon-induced constipation in mice[J].Experimental and Therapeutic Medicine, 2015, 61 (9) :272-278.
[10]	冯霞, 赵欣.不同容器发酵水豆豉预防CCl4诱导肝损伤的研究[J].现代食品科技, 2016, 37 (7) :338-342.
[11]	Strober W, Fuss IJ, Blumberg RS.The immunology of mucosal models of inflammation[J].Annual Review of Immunology, 2002 (20) :495-549.
[12]	Mustafa A, El-Medany A, Hagar HH, et al.Ginkgo biloba attenuates mucosal damage in a rat model of ulcerative colitis[J]. Pharmacological Research, 2006, 53 (4) :324-330.
[13]	Osman N, Adawi D, AhrnéS, et al.Probiotics and blueberry attenuate the severity of dextran sulfate sodium (DSS) -induced colitis[J].Digesrive Disease and Sciences, 2008, 53 (9) :2464-2473.
[14]	Fiocchi C.Inflammatory bowel disease:new insights into mechanisms of inflammation and increasingly customized approaches to diagnosis and therapy[J].Current Opinion in Gastroenterology, 2004, 20 (4) :309-310.
[15]	周国胜.一氧化氮与炎症性肠病[J].安徽医药, 2002, 12 (11) :1010-1012.
[16]	李慧, 陆宗海, 陈蕾, 等.溃疡性结肠炎小鼠结肠Cajal间质细胞及干细胞因子的变化[J].南京医科大学学报:自然科学版, 2009, 29 (5) :648-651.
[17]	Galli SJ, Tsai M, Wershil BK.The c-kit receptor, stem cell factor, and mast cells.What each is teaching us about the others[J].American Journal of Pathology, 1993, 142 (4) :965-974.
[18]	朱锋, 王晓兵, 刘适, 等.CXC趋化因子受体2和白细胞介素-8在炎症性肠病患者中的表达及其意义[J].胃肠病学, 2016, 21 (6) :331-335.

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植物乳杆菌YS-1对恶唑酮诱导BALB/c小鼠结肠炎的预防作用

作者简介: 孙鹏 (1983-) , 男, 硕士, 讲师, 研究方向:食品营养和功能性食品, E-mail:598535244@qq.com。; 赵欣 (1981-) , 男, 博士, 教授, 研究方向:食品营养和功能性食品, E-mail:zhaoxin@cque.edu.cn。;

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Preventive effects of Lactobacillus plantarum YS-1 on oxazolone-induced BALB/c mice colitis

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作者简介:
孙鹏 (1983-) , 男, 硕士, 讲师, 研究方向:食品营养和功能性食品, E-mail:598535244@qq.com。;

赵欣 (1981-) , 男, 博士, 教授, 研究方向:食品营养和功能性食品, E-mail:zhaoxin@cque.edu.cn。;