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中国精品科技期刊2020

三嗪类除草剂免疫分析技术的研究进展

韩正政, 刘媛, 王健, 张俊花, 黄伟

韩正政, 刘媛, 王健, 张俊花, 黄伟. 三嗪类除草剂免疫分析技术的研究进展[J]. 食品工业科技, 2017, (16): 324-329. DOI: 10.13386/j.issn1002-0306.2017.16.061
引用本文: 韩正政, 刘媛, 王健, 张俊花, 黄伟. 三嗪类除草剂免疫分析技术的研究进展[J]. 食品工业科技, 2017, (16): 324-329. DOI: 10.13386/j.issn1002-0306.2017.16.061
HAN Zheng-zheng, LIU Yuan, WANG Jian, ZHANG Jun-hua, HUANG Wei. Research progress of immunoassay on the detection of triazine herbicides[J]. Science and Technology of Food Industry, 2017, (16): 324-329. DOI: 10.13386/j.issn1002-0306.2017.16.061
Citation: HAN Zheng-zheng, LIU Yuan, WANG Jian, ZHANG Jun-hua, HUANG Wei. Research progress of immunoassay on the detection of triazine herbicides[J]. Science and Technology of Food Industry, 2017, (16): 324-329. DOI: 10.13386/j.issn1002-0306.2017.16.061

三嗪类除草剂免疫分析技术的研究进展

基金项目: 

河北省现代农业技术体系蔬菜产业创新团队建设项目(HBCT2013050208); 国家公益性行为(农业)科研专项(201203094);

详细信息
    作者简介:

    韩正政 (1990-) , 男, 硕士研究生, 研究方向:农药残留检测技术, E-mail:498233705@qq.com。;

    王健 (1980-) , 男, 博士, 副教授, 研究方向:食品安全检测技术, E-mail:xuanyuanjian0228@126.com。;

  • 中图分类号: TQ450.7

Research progress of immunoassay on the detection of triazine herbicides

  • 摘要: 在针对三嗪类除草剂的检测中,免疫分析技术的发展已越来越受到社会的关注,并成为研究的热点。本文主要介绍了免疫分析技术在三嗪类除草剂检测中的几个关键技术环节,即半抗原及人工抗原的设计合成、抗体的制备以及各种免疫分析技术在三嗪类除草剂检测中的应用,并对目前存在的问题和今后的发展方向进行了讨论。 
    Abstract: In the detection of triazine herbicides, the development of immunoassay has gotten more and more social attention and it has been the research hotspots. In this study, several key steps of immunoassay on the determination of triazine herbicides were introduced.These steps contained design and synthesis of hapten and artificial antigen, preparation of antibody and the application of many kinds of immunoassay on the detection of triazine herbicides.Besides, the current problems and the prospect of the field were also discussed.
  • [1]

    Baranowska I, Barchanska H, Pacak E.Procedures of trophic Chain samples preparation for determination of triazines by HPLC and metals by ICP-AES methods[J].Environ Pollut, 2006, 143 (2) :206-211.

    [2] 王晓春, 王广, 焦杏春.三嗪类除草剂分析方法研究进展[J].农药, 2011, 50 (5) :320-324.
    [3] 黎其万, 潘灿平.农药残留免疫分析方法及其应用研究进展[J].西南农业学报, 2004, 1 (2) :248-252.
    [4] 杨依军, 王勇, 杨秀荣, 等.免疫分析法在农药残留分析中的应用[J].华北农学报, 2001, 16 (4) :119-124.
    [5] 郑晓冬, 何丹.食品中农药残留免疫检测技术的研究进展[J].中国食品学报, 2004, 4 (2) :88-94.
    [6]

    Hammock B D, Mumma R O.Recent advances in pesticide analytical methodology[J].Journal of the American Chemical Society, 1980, 321-352.

    [7]

    Huber S.Improved solid-phaseenzyme immunoassay systems in the ppt range for atrazinin fresh water[J].Chemosphere, 1985, 14 (11-12) :1795-1803.

    [8] Jung F, 黄葵.免疫分析技术在农药分析中的应用[J].国外农业环境保护, 1991 (4) :17-19.
    [9] 汪尔康.21世纪的分析化学[M].北京:科学出版社, 2001:269-284.
    [10]

    Sun P, Kobayashi N, Goto J.Design and synthesis of6α-Corticoste-roid haptens and their bovine serum albumin (BSA) conjugates[J].Chinese Chemical Letters, 2003, 14 (3) :259-2621.

    [11]

    Brum E M, Garcés-García M, Puchades R, et al.Highly sensitive enzyme-linked immunosorbent assay for chlorpyrifos application to olive oil analysis[J].Journal of Agricultural and Food Chemistry, 2005, 53 (24) :9352-9360.

    [12] 范祚舟, 徐加发, 等.酶联免疫分析技术研究进展[J].分析科学学报, 2011, 27 (1) :113-118.
    [13]

    M H Goodrow, R O Harrison, B D Hammock.Hapten synthesis, antibody development, and competitive inhibition enzyme immunoassay for s-triazine herbicides[J].Journal of Agricultural and Food Chemistry, 1990, 38 (4) :990-996.

    [14]

    J Schlaeppi, W F9ry, K Ramsteiner.Hydroxyatrazine and atrazine determination in soil and water by enzyme-linked immunosorbent assay using specific monoclonal antibodies[J].Journal of Agricultural and Food Chemistry, 1989, 37 (6) :1532-1538.

    [15]

    R O Harrison, M H Goodrow, B D Hammock.Competitive inhibition ELISA for the s-triazine herbicides:assay optimization and antibody characterization[J].Journal of Agricultural and Food Chemistry, 1991, 39 (1) :122-128.

    [16]

    K Charlton, W J Harris, A J Porter.The isolation of supersensitive anti-hapten antibodies from combinatorial antibody libraries derived from sheep[J].Biosensors&Bioelectronics, 2001, 16 (9-12) :639-646.

    [17]

    Wie S I, Hammock B D.Development of enzyme-linked immunosorbent assays for residue analysis of diflubenzuron and BAY SIR 8514[J].Journal of Agricultural and Food Chemistry, 1982, 3 (5) :949-957.

    [18]

    Karl Kramer.Synthesis of a group-selective antibody library against haptens[J].Journal of Immunological Methods, 2002, 266 (1-2) :209-220.

    [19]

    Qie Z, Bai J, Xie B, et al.Sensitive detection of atrazine in tap water using TELISA[J].Analyst, 2015, 140 (15) :5220-5226.

    [20]

    Belkhamssa N, Justino CI, Santos PS, et al.Label-free disposable immunosensor for detection of atrazine[J].Talanta, 2015, 146 (1) :430-434.

    [21]

    LV Zhi Qiang, WANG Cai Hong, WANG Ting Ting, et al.Detection of Atrazine Residue in Food Samples by a Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay[J].Biomedical and Environmental Sciences, 2013, 26 (5) :389-402.

    [22]

    Won-Bo Shim, Zheng-You Yang, Ji-Young Kim, et al.Immunochromatography Using Colloidal Gold-Antibody Probe for the Detection of Atrazine in Water Samples[J].Journal of Agricultural and Food Chemistry, 2006, 54 (26) :9728-9734.

    [23] 李伶伶.三嗪类除草剂扑草净快速免疫检测方法的研究[D].天津:天津科技大学, 2012.
    [24]

    Sonia Herranz, Javier Ramón-Azcón, Elena Benito-Pe1a, et al.Preparation of antibodies and development of a sensitive immunoassay with fluorescence detection for triazine herbicides[J].Analytical and Bioanalytical Chemistry, 2008, 391 (5) :1801-1812.

    [25] 柳明, 祁军, 刘磊, 等.SPR免疫传感技术检测水中阿特拉津除草剂[J].解放军预防医学杂志, 2013, 31 (4) :299-301.
    [26]

    Leif Bruun, Claus Koch, Mogens Havsteen Jakobsen, et al.Characterization of monoclonal antibodies raised against different structures belonging to the s-triazine group of herbicides[J].Analytica Chimica Acta, 2001, 436 (1) :87-101.

    [27] 徐德武, 朱永清.用放射免疫测定法分析水中莠去津残留量的研究[J].莱阳农学院学报, 2000, 17 (3) :225-228.
    [28]

    Eline P Meulenberg, L G de Vree, J Dogterom.Investigation of indicative methods in the Netherlands:validation of several commercial ELISAs for pesticides[J].Analytica Chimica Acta, 1999, 399 (1-2) :143-149.

    [29]

    Jasdeep Kaur, Robin C Boro, Nishima Wangoo, et al.Direct hapten coated immunoassay format for the detection of atrazine and 2, 4-dichlorophenoxyacetic acid herbicides[J].Analytica Chimica Acta, 2008, 607 (1) :92-99.

    [30]

    Hanna Barchanska, Elzbieta Jodo, Robert Graham Price, et al.Monitoring of atrazine in milk using a rapid tube-based ELISA and validation with HPLC[J].Chemosphere, 2012, 87 (11) :1330-1334.

    [31]

    Elena V Yazynina, Anatoliy V Zherdev, Boris B Dzantiev, et al.Microplate immunoassay technique using polyelectrolyte carriers:kinetic studies and application to detection of the herbicide atrazine[J].Analytica Chimica Acta, 1999, 399 (1-2) :151-160.

    [32] 单国民, 钱传范, 过琴媛, 等.三氮苯类除草剂的酶联免疫吸附测定[J].中国农业大学学报, 1996, 1 (3) :52-58.
    [33] 邓安平, Milan F.酶联免疫吸附分析法测定水样中的阿特拉津[J].分析化学, 1998, 26 (1) :29-33.
    [34] 邓安平, 杨红, Milan F.酶联免疫吸附分析法测定土壤试样中的阿特拉津[J].分析化学, 1999, 27 (6) :657-660.
    [35] 胡晓航, 王皙玮, 吴玉梅, 等.酶联免疫法测定土壤中莠去津[J].中国糖料, 2011 (1) :28-30.
    [36]

    Reimer G J, Gee S J, Hammock B D.Comparison of a timeresolved fluorescence immunoassay and an enzyme-linked immunosorbent assay for the analysis of atrazine in water[J].Journal of Agricultural and Food Chemistry, 1998, 46 (8) :3353-3358.

    [37]

    Choi M J, Lee J R, Eremin S A.Development of single reagent for fluorescence polarization immunoassay of atrazine[J].Food and Agricultural immunology, 2002, 14 (2) :107-120.

    [38]

    Schobel U, Egelhaaf H J, Brecht A, et al.New donoracceptor pair for fluorescent immunoassays by energy transfer[J].Bioconjugate Chemistry, 1999, 10 (6) :1107-1114.

    [39]

    Matveeva E G, Melik-Nubarov N S, Miethe P, et al.Antigen-antibody interactions in the reverse micellar system:quenching of the fluorescence of fluorescein-labeled atrazine by antibodies against atrazine[J].Analytical Biochemistry, 1996, 234 (1) :13-18.

    [40]

    Jeanne V Samsonova, Maya Yu Rubtsova, Anna V Kiseleva, et al.Chemiluminescent multiassay of pesticides with horseradish peroxidase as a label[J].Biosensors&Bioelectronics, 1999, 14 (3) :273-281.

    [41]

    Tudorache M, Tencaliec A, Bala C.Magnetic bead-based immunoassay as a sensitive alternative for atrazine analysis[J].Talanta, 2008, 77 (2) :839-8431.

    [42]

    Koivunen M E, Dettmer K, Vermeulen R, et al.Improved methods for urinary atrazine mercapturate analysis—Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE) [J].Analytica Chimica Acta, 2006, 572 (2) :180-189.

    [43]

    Hongyan Zhang, Shuo Wang, Guozhen Fang.Applications and recent developments of multi-analyte simultaneous analysis by enzyme-linked immunosorbent assays[J].Journal of Immunological Methods, 2011, 368 (1-2) :1-23.

    [44]

    L Campanella, S Eremin, D Lelo, et al.Reliable new immunosensor for atrazine pesticide analysis[J].Sensors and Actuators b B:Chemical, 2011, 156 (1) :50-62.

    [45]

    S Hleli, C Martelet, A Abdelghani, et al.Atrazine analysis using an impedimetric immunosensor based on mixed biotinylated self-assembled monolayer[J].Sensors and Actuators B:Chemical, 2006, 113 (2) :711-717.

    [46]

    Ramón-Azcón J, Kunikata R, Sanchez F J, et al.Detection of pesticide residues using an immunodevice based on negative dielectrophoresis[J].Biosensors and Bioelectronics, 2009, 24 (6) :1592-1597.

    [47] 严智燕, 周立群.生物传感器在农药残留检测中的应用[J].农药研究与应用, 2010, 14 (3) :6-10.
    [48]

    Milan Fránek, Anping Deng, Vladimír Koláǐ.Performance characteristics for flow injection immunoassay using monoclonal antibodies against s-triazine and 2, 4-D herbicides[J].Analytica Chimica Acta, 2000, 412 (1-2) :19-27.

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  • 收稿日期:  2017-01-10

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