大豆油酶法精炼的应用实践

基金项目:

广东省产学研项目(2013B090200015)；

详细信息

Application of enzymatic refining in soybean oil

摘要: 以水化脱胶大豆油为原料,选用磷脂酶lecitase ultra进行小样实验,单因素实验结果表明最优的反应条件为:酶添加量30 mg/kg,加水量3.0%,p H5.0,反应温度50℃,反应时间5 h。在此基础上,进行了800 t/d连续式酶法精炼工厂化实验,生产实践表明酶法成品油得率较化学精炼法提高0.8%,经济效益明显,同时对比了酶法精炼成品油与化学精炼成品油的理化指标及稳定性,两者无明显差异。
- 酶法精炼 /
- 大豆油 /
- 得率 /
- 稳定性
Abstract: The laboratory experiments were conducted with hydration degumming soybean oil as raw material by the phospholipase lecitase ultra.The single factor results showed that the optimum reaction conditions were as follows:enzyme dosage of 30 g/t oil, water content of 3.0%, p H5.0, reaction temperature of 50℃, reaction time of 5 h.Under these conditions, the continuous enzymatic degumming production experiments were carried out on the 800 t/d production line.The results showed that the oil yield increased by 0.8%compared to the chemical method, it showed obvious economic benefits.The physical and chemical indexes and stability of refined oils produced by both methods were compared, and the results showed no obvious difference.
- enzymatic refining /
- soybean oil /
- yield /
- stability

[1]	杨博, 王宏建.经济环保的酶法脱胶技术[J].中国油脂, 2004, 29 (3) :21-23.
[2]	Buchold H, Boensch R, Schroeppel J.Process for enzymatically degumming vegetable oil:, US5558781[P].1996.
[3]	杨继国, 杨博, 周2) , 等.酶法脱胶过程反应机理的研究[J].中国油脂, 2007, 32 (9) :35-37.
[4]	潘明喆, 柴玉华, 杜鹏.磷脂酶A_1用于大豆油酶法脱胶技术的研究[J].食品工业科技, 2008, (5) :219-222.
[5]	罗淑年, 于殿宇, 韩锋, 等.酶法脱胶物理精炼大豆油[J].食品科学, 2007, 28 (10) :287-289.
[6]	叶展, 冉玉斌, 何东平, 等.菜籽油磷脂酶C脱胶与水化脱胶条件优化及效果对比研究[J].食品工业科技, 2015, 36 (14) :260-265.
[7]	于殿宇, 马莺, 张欢, 等.磁性固定化PLA1的制备及其在脱胶应用中的研究[J].食品工业科技, 2014, 35 (19) :128-131.
[8]	冯晓阳, 冯晓明.大豆油酶法脱胶条件的优化[J].中外食品工业月刊, 2015, (3) :4-5.
[9]	段书平.大豆油酶法脱胶的生产应用[J].中国油脂, 2013, 38 (8) :11-13.
[10]	杨亚济.大豆油酶法脱胶应用实践[J].中国油脂, 2016, 41 (8) :107-109.
[11]	Seibert E, Tracy T S.Fundamentals of Enzyme Kinetics[J].The Quarterly Review of Biology, 1997, 116 (Volume 72, Number3) :9-22.
[12]	Klibanov A M.Enzyme-Catalyzed Processes in Organic Solvents[J].Proceedings of the National Academy of Sciences of the United States of America, 1985, 82 (10) :3192.