Mechanisms and effects of calycosin on learning-memory function in aging mice
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摘要: 目的:研究毛蕊异黄酮对D-半乳糖致衰老小鼠学习记忆能力的改善作用及作用机制。方法:小鼠皮下注射D-半乳糖150 mg·kg-1·d-1,连续6周,造模后的小鼠分为模型组、奥拉西坦组(300 mg·kg-1·d-1)、和毛蕊异黄酮高、中、低剂量组(20、12、7 mg·kg-1·d-1)。模型组和正常对照组小鼠给予等剂量的生理盐水。处理7 d后,利用跳台和Y迷宫实验检测空间记忆和被动学习能力,测定肝、脾、胸腺和脑器官指数,检测血清总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）含量和脑组织中乙酰胆碱酯酶（Ach E）活力。结果表明,与模型组相比,毛蕊异黄酮处理组小鼠在Y迷宫中正确反应次数和主动回避率明显增加（p<0.05）,在跳台实验中逃避期均明显缩短,错误次数明显减少（p<0.05）;毛蕊异黄酮处理组小鼠肝、脾、胸腺和脑器官指数与正常对照组没有明显差异;抗氧化酶（T-SOD、GSH-Px）活力显著升高（p<0.05）,MDA含量明显降低,脑组织中Ach E活性降低（p<0.05）。结论:毛蕊异黄酮对D-半乳糖致衰老小鼠学习记忆能力有改善作用,毛蕊异黄酮可能是通过提高内源抗氧化酶（T-SOD、GSH-Px）活力,降低脂质过氧化损伤,保护脑、脾、胸腺等器官功能和降低脑组织Ach E活性来提高D-半乳糖致衰老小鼠的学习记忆能力。Abstract: Objective: The effects of calycosin on learning-memory behavior in aging mice induced by D-galactose were investigated in this study. Methods: The aging mice model was induced by subcutaneous injection of D-galactose ( 150 mg·kg-1·d-1) were established after 6 weeks.The D-galactose-induced aging mice were divided into model group, oxiracetam group ( 300 mg·kg-1·d-1) , and calycosin high, medium and low dose treated-groups ( 20, 12, 7 mg·kg-1·d-1) .Mice in the model group and normal control group were given normal saline. After intraperitoneal injection 7 d, the passive learning ability and memory capacity of mouse were investigated by the platform and the Y maze. Then organ index and antioxidant activity were detected, such as liver, spleen, thymus, brain and serum superoxide dismutase ( T-SOD) , glutathione peroxidase ( GSH-Px) , malondialdehyde ( MDA) and brain acetylcholinesterase ( Ach E) activity. Results: Compared with the model group, in the Y maze, the number of correct reaction and active avoidance rate of calycosin-treated mice were significantly increased ( p < 0.05) , and the escape period was significantly shortened and the number of errors in the step-down test was significantly reduced ( p < 0.05) . Compared with the model group, liver, spleen, thymus and brain organ index of calycosin-treated mice were significantly different ( p < 0.05) .In the calycosin groups, antioxidant enzyme ( T-SOD, GSH-Px) activity was significantly elevated ( p < 0.05) , MDA content was significantly reduced ( p < 0.05) , and brain tissue Ach E activity was decreased ( p < 0.05) . Conclusion: Calycosin can improve the learning-memory function of aging mice induced by D-galactose. The mechanism may be to increase the endogenous antioxidant enzyme activity, reduce lipid peroxidation damage, protect organ function such as brain, spleen and thymus and reduce brain tissue Ach E activity.
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Keywords:
- calycosin /
- D-galactose /
- organ index /
- antioxidation /
- anti-aging
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