Optimization of extraction technology of dietary fiber from Oenanthe javanica and its character
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摘要: 以水芹为试材,采用酶和化学结合法提取其膳食纤维,在单因素实验基础上,利用正交实验优化水芹膳食纤维提取工艺,最后对水芹膳食纤维持水力和膨胀力进行评价。结果表明,最佳酶解工艺条件为酶底比40∶1 U/g、酶解温度50℃、酶解时间1.5 h,最佳碱解工艺条件为液料比30∶1(m L/g)、碳酸钠浓度2.5%、碱解温度30℃、碱解时间1 h,在此条件下水芹总膳食纤维(TDF)提取率为47.94%,其中水溶性膳食纤维(SDF)为4.78%,不溶性膳食纤维为43.16%,同时发现水芹叶中膳食纤维含量高于茎秆,且SDF比例较高。水芹TDF的膨胀力和持水力分别达到6.27 m L/g和389%,且水芹叶TDF的膨胀力和持水力高于水芹茎TDF,可能是由水芹叶TDF中SDF占比例较高所致。Abstract: This study aimed to optimize the extraction technology of dietary fiber from Oenanthe javanica using single-factor experiment and orthogonal array. Combined method of enzymolysis and alkaline hydrolysis were applied to optimize the extraction of dietary fiber, and the water holding capacity and swelling capacity of extracted dietary fiber were evaluated. The optimal enzymolysis conditions were determined as follows: enzyme-to-substrate ratio, 40 ∶ 1 U/g, enzymolysis temperature50 ℃, enzymolysis time 1.5 h. The optimal alkaline hydrolysis conditions were determined as follows: liquid-to-solid ratio30∶ 1 ( m L/g) , sodium carbonate concentration 2.5%, alkaline hydrolysis temperature 30 ℃, alkaline hydrolysis time 1 h.Under these conditions, the extraction rate of total dietary fiber ( TDF) , soluble dietary fiber ( SDF) , insoluble dietary fiber were47.94%, 4.78% and 43.16%, respectively.The content of dietary fiber in leaf of O.javanica was much higher than in stem, and SDF also possessed higher proportion in TDF from leaf.The water holding capacity and swelling capacity of TDF from O.javanica were 389%, 6.27 m L/g, respectively.TDF from leaf had higher water holding capacity and swelling capacity than those in stem, probably because of the higher proportion of SDF.
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Keywords:
- Oenanthe javanica /
- dietary fiber /
- extraction /
- character
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[1] 王彦玲, 刘冬, 付全意, 等.膳食纤维的国内外研究进展[J].中国酿造, 2008, 27 (5) :1-4. [2] Snchez-tena S, Lizarraga D, Miranda A, et al.Grape antioxidant dietary fiber inhibits intestinal polyposis in Apc Min/+mice:relation to cell cycle and immune response[J].Carcinogenesis, 2013, 34 (8) :1881-1888.
[3] Hanan M A, Ahmed A R.Utilization of watermelon rinds and sharlyn melon peels as a natural source of dietary fiber and antioxidants in cake[J].Annals of Agricultural Sciences, 2013, 58 (1) :83-95.
[4] Crescenti A, Sol R, Valls R M, et al.Polymorphisms in LEP and NPY genes modify the response to soluble fiber Plantago ovata husk intake on cardiovascular risk biomarkers[J].Genes and Nutrition, 2013, 8 (1) :127-136.
[5] Castellanos-jankiewicz A, Bosque-plata L D, Tejero M E.Combined effect of plant sterols and dietary fiber for the treatment of hypercholesterolemia[J].Plant Foods for Human Nutrition, 2014, 69 (2) :93-100.
[6] de Luis román, la Fuente B D, Jáuregui O I, et al.Double blind randomized clinical trial controlled by placebo with an alpha linoleic acid and prebiotic enriched cookie on risk cardiovascular factor in obese patients[J].Nutricion Hospitalaria, 2011, 26 (4) :827-833.
[7] Kitano Y, Murazumi K, Duan J J, et al.Effect of dietary porphyrin from the red alga, porphyra yezoensis, on glucose metabolism in diabetic KK-Ay mice[J].Journal of Nutritional Science and Vitaminology, 2012, 58 (1) :14-19.
[8] Sohn V R, Giros A, Xicola R M, et al.Stool-fermented Plantago ovata husk induces apoptosis in colorectal cancer cells independently of molecular phenotype[J].British Journal of Nutrition, 2012, 107 (11) :1591-1602.
[9] Zeng H W, Lazarova D L, Bordonaro M.Mechanisms linking dietary fiber, gut microbiota and colon cancer prevention[J].World Journal of Gastrointestinal Oncology, 2014, 6 (2) :41-51.
[10] Prez-jimnez J, Diaz-rubio M, Mesias M, et al.Evidence for the formation of maillardized insoluble dietary fiber in bread:a specific kind of dietary fiber in thermally processed food[J].Food Research International, 2014, 55:391-396.
[11] Foschia M, Peressini D, Sensidoni A, et al.The effect of dietary fiber addition on the quality of common cereal products[J].Journal of Cereal Science, 2013, 58 (2) :216-227.
[12] Eim V S, Simal S, Rossell C, et al.Optimisation of the addition of carrot dietary fiber to a dry fermented sausage sobrassada using artificial neural networks[J].Meat Science, 2013, 94 (3) :341-348.
[13] 李银姬, 朴惠善.水芹的研究进展[J].延边医学院学报, 1996, 19 (4) :243-245. [14] 黄正明.有关中草药水芹现代研究的若干探讨[J].世界华人消化杂志, 2001, 9 (1) :1-5. [15] 黄正明, 杨新波, 曹文斌, 等.中药水芹的药用研究[J].中国药理通讯, 2003, 20 (1) :25-26. [16] Lee C H, Park J H, Cho J H, et al.Effect of Oenanthe javanica extract on antioxidant enzyme in the rat liver[J].Chinese Medical Journal, 2015, 128 (12) :1649-1654.
[17] Yang X B, Huang Z M, Cao W B, et al.Antidiabetic effect of Oenanthe javanica flavone[J].Acta Pharmacologica Sinica, 2000, 21 (3) :239-242.
[18] 刘焕云, 李慧荔, 赵红.燕麦麸中水溶性膳食纤维提取工艺优化[J].农业机械学报, 2008, 39 (7) :103-106. [19] Wan Y T, Rodezno L A E, Solval K M, et al.Optimization of soluble dietary fiber extraction from defatted rice bran using response surface methodology[J].Journal of Food Processing and Preservation, 2014, 38 (1) :441-448.
[20] Jing Y, Chi Y J.Effect of twin-screw extrusion on soluble dietary fiber and physicochemical properties of soybean residue[J].Food Chemistry, 2013, 138 (2) :884-889.
[21] 令博, 田云波, 吴洪斌, 等.微生物发酵法制取葡萄皮渣膳食纤维的工艺优化[J].食品科学, 2012, 33 (15) :178-182. [22] Li X L, He X L, LüY P, et al.Extraction and functional properties of water-soluble dietary fiber from apple pomace[J].Journal of Food Process Engineering, 2014, 37 (3) :293-298.
[23] 刘红开, 李放, 张亚宏, 等.不同品种蚕豆种皮中膳食纤维的提取工艺优化及其理化特性[J].食品科学, 2016, 37 (16) :22-28. [24] 李来好, 杨贤庆, 吴燕燕, 等.用正交设计法提取马尾藻高活性膳食纤维[J].湛江海洋大学学报, 1998, 18 (2) :39-43. [25] 李敏, 李含晰, 周韬, 等.纤维素酶提取刺葡萄膳食纤维的工艺研究[J].湖南农业科学, 2012 (19) :99-101.
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