Effect of Lactobacillus Plantarum KLDS 1.0386on the cholesterol metabolism in C57BL/6 mice
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摘要: 目的:本研究测定了植物乳杆菌KLDS 1.0386对模拟胃肠液的耐受力,并通过构建高脂血症模型小鼠评价该菌株对小鼠体内胆固醇代谢的影响。方法:体外模拟胃液肠液,菌落计数法检测胃液肠液耐受性,并以健康C57BL/6雄性小鼠为对象,设置空白组、模型组、低剂量组、高剂量组、普伐他汀对照组,分别以生理盐水、KLDS 1.0386悬浊液和普伐他汀连续灌胃4周,检测小鼠肝脏胆固醇和甘油三酯,粪便胆汁酸和胆固醇水平。结果:在p H为3的模拟胃液环境中,该菌株3 h存活率可达92.62%,在模拟肠液环境中,3 h存活率为83.91%,说明植物乳杆菌KLDS 1.0386能通过胃进入肠道并保持活性。植物乳杆菌KLDS 1.0386菌株可显著降低高脂小鼠肝脏甘油三酯和胆固醇水平(p<0.05),增加粪便胆汁酸和胆固醇的排出(p<0.05)。结论:该菌可以用做降胆固醇的潜在益生菌。Abstract: Objective: It was evaluated that the effect of Lactobacillus plantarum KLDS strain on the cholesterol metabolism in hyperlipidemia mice and zhe ability under simulated gastric and intestinal juice. Method: Viable count was used to detect the tolerance characteristics under simulated gastric and intestinal juice environment.Health C57 BL/6 male mice, as objects, were divided into control group, model group, low-dose group, high-dose group and pravastatin group, which were respectively given normal saline, Lactobacillus plantarum KLDS 1.0386 bacteria suspension and pravastatin solution by gavage for 4 consecutive weeks.The liver levels of total cholesterol ( TC) , triglyceride ( TG) and the feces levels of TC, total bile acid ( TBA) were determined on the 28 th day of the experiment.Results: It showed that the tolerance rate in gastric juice p H3.0 was 92.62%, and the intestinal juice was 83.91%, so, Lactobacillus plantarum KLDS 1.0386 can pass the stomach into the intestines and keep active in the gut. After 4 weeks administration, compared with mice fed a high-cholesterol diet without lactic acid bacteria supplementation, there was a significant ( p < 0.05) decrease in triglyceride and total cholesterol levels in the hepatic of mice treated with KLDS 1.0386 strain. Furthermore, total cholesterol and total bile acid levels in the feces were significantly ( p <0.05) increased after KLDS 1.0386 administration.Concluton: Lactobacillus plantarum KLDS 1.0386 could be as the probiotics with cholesterol-lowering activity.
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Keywords:
- Lactobacillus plantarum /
- gastric juice /
- intestinal juice /
- cholesterol /
- bile acid
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