Extraction of water soluble protein from selenium contained Pleurotus ostreatus and antioxidant activity in vitro
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摘要: 在提取温度、提取时间、料液比单因素实验研究基础上结合三因素三水平的正交实验对富硒平菇水溶性蛋白的提取工艺进行优化实验,并对不同浓度下水溶性蛋白的抗氧化性进行了测定。结果表明:水溶性蛋白提取的最优条件为:提取温度为30℃,料液比为1∶30,提取时间4 h,在此条件下提取得到的水溶性蛋白浓度为(8.5±0.29)mg/mL。水溶性蛋白具有清除超氧阴离子自由基、羟自由基、ABTS阳离子自由基的能力,浓度为1.0 mg/mL时对自由基的清除率可达到50%以上。Abstract: In this experiment, the main research material was dried selenium-enriched Pleurotus ostreatus, in order to get optimization of extraction technology for water soluble protein and determine its antioxidant activity in vitro, single factors and orthogonal experiments were designed. The results showed that the best conditions for extraction were described as follows:extraction temperature 30 ℃, extraction time 4 h and the ration of raw material to water 1∶ 30, under this conditions, the content of water soluble protein reached ( 8.5 ± 0.29) mg/mL.The water soluble protein had the capacity of scavenging superoxide anion free radical, hydroxyl radical and ABTS radical, and the scavenging rate could get higher than 50% at the concentration of 1.0 mg/mL.
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Keywords:
- Se enriched Pleurotus ostreatus /
- selenoprotein /
- extraction conditions /
- antioxidant
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