应用多重实时荧光PCR检测蜡样芽胞杆菌毒力基因

刘芳; 陈国培; 王远洋; 郭正洋; 陈晶; 刘钟栋; 兰全学

doi:10.13386/j.issn1002-0306.2018.02.048

应用多重实时荧光PCR检测蜡样芽胞杆菌毒力基因

1. 深圳市计量质量检测研究院, 广东深圳 518000;
2. 河南工业大学, 河南郑州 450000

基金项目:

深圳市科技创新委员会基金资助项目(JCYJ20150625100647056)。

详细信息

作者简介:
刘芳(1982-),女,硕士,工程师,研究方向:食品微生物学检验,E-mail:4275777@qq.com。

通讯作者:
兰全学(1977-),男,硕士,高级工程师,研究方向:卫生检验与食品安全风险管理,E-mail:61723585@qq.com。

中图分类号: TS207.4
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出版历程
- 收稿日期: 2017-06-27
- 网络出版日期: 2020-12-25
- 刊出日期: 2018-01-14

Development of a mutiplex real-time PCR assay for detection of the toxin gene to Bacillus cereus

1. Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518000, China;
2. Henan University of Technology, Zhengzhou 450000, China

摘要

摘要: 目的:建立一种快速、准确、简便的蜡样芽胞杆菌毒力基因检测方法,为蜡样芽胞杆菌更深入的研究提供依据和便利。方法:采用TaqMan探针法设计了三重hblA,hblC,hblD基因,三重nheA,nheB,nheC基因,三重ces,entFM,bceT基因的多重实时荧光PCR体系检测蜡样芽胞杆菌毒力基因。结果:多重体系对蜡样芽胞杆菌毒力基因的检测具有良好的特异性和灵敏性,其最低检出限为63 CFU/mL。结论:利用上述体系对蜡样芽胞杆菌的毒力基因进行检测能够省时省力,特异性、灵敏性良好,具有实际应用价值。
- 蜡样芽胞杆菌 /
- 多重TaqMan实时荧光PCR /
- 毒力基因
Abstract: Objective:To develop a fast,accurate and simple method to detect the toxin genes in Bacillus cereus,and provide basis and convience for a deeper research. Methods:Three multiplex real-time fluorescence PCR systems were developed by TaqMan Probe method for the detection of toxin genes of hblA-hblC-hblD for the first PCR reaction system,nheA-nheB-nheC for the second,entFM-bceT-ces for the third. Results:The detection results of toxin genes in Bacillus cereus indicated excellent specificity and sensitivity,detection of limit of bacterial concentration was 63 CFU/mL. Conclution:The systems above had the characteristics of time saving and labor saving. They had excellent specificity and sensitivity.
- Bacillus cereus /
- multiplex real-time fluorescence PCR /
- toxin gene