鳕鱼卵中磷脂提取工艺的优化

李晓彬; 杨书庆; 吕宪峰; 张姗姗; 张轩铭; 何秋霞; 侯海荣; 刘可春; 韩利文

doi:10.13386/j.issn1002-0306.2018.03.035

鳕鱼卵中磷脂提取工艺的优化

1. 齐鲁工业大学(山东省科学院), 山东省科学院生物研究所, 山东省科学院药物筛选技术重点实验室, 山东省生物检测技术工程实验室, 山东济南 250103;
2. 山东师范大学生命科学学院, 山东济南 250014;
3. 蓬莱海洋(山东)股份有限公司, 山东蓬莱 265607

基金项目:

国家海洋公益性行业科研专项经费项目(201505030-2)

山东省重点研发计划项目(2016YYSP004)

烟台市科技计划项目(2016YTZD0007)

山东省三院联合基金项目(ZR2015YL009)。

山东省重点研发计划项目(2016GSF121009)

详细信息

作者简介:
李晓彬(1985-),女,博士,助理研究员,研究方向:海洋天然活性成分的发现和制备,E-mail:bin85666666@163.com。

通讯作者:
韩利文(1980-),男,博士,副研究员,研究方向:天然活性成分的发现和制备,E-mail:hanliwen08@126.com。

中图分类号: TS201.1
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出版历程
- 收稿日期: 2017-07-19
- 网络出版日期: 2020-12-25
- 刊出日期: 2018-01-31

Optimization of extraction process for phospholipids from codfish roe

1. Biology Institute, Qilu University of Technology(Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Ji'nan 250103, China;
2. College of Life Science, Shandong Normal University, Ji'nan 250014, China;
3. Penglai Marine(Shandong) Co., Ltd., Penglai 265607, China

摘要

摘要: 为了充分利用鳕鱼资源,提高海产品附加值,采用溶剂提取法,以乙醇液为提取溶剂,以磷脂得率及纯度为考察指标,通过单因素实验及正交实验,探讨乙醇浓度、料液比、提取次数和提取时间等四个因素对磷脂提取的影响,并制定出最优鳕鱼卵中磷脂的提取工艺。结果表明,鳕鱼卵中磷脂的最佳提取工艺条件为:以1:10 (g/mL)的料液比比例,采用乙醇浓度为90%的乙醇溶剂搅拌提取鳕鱼卵3 次,每次提取6 h。该工艺成本低、方法简单、污染少、易于实现工业化。
- 鳕鱼卵 /
- 正交实验 /
- 磷脂 /
- 提取工艺
Abstract: In order to make full use of the codfish resources and improve the added value of seafood,the extraction process of phospholipids in cod roe was optimized and studied by solvent extraction(ethanol as the extraction solvent). The effects of four factors(ethanol concentration,ratio of material to liquid,the extraction time and the number of extraction)on the yield and phospholipid purity of extracts were researched to determine the optimum extraction process of cod roe through single factor study and orthogonal experimental analysis. The results showed the optimal extraction conditions were as follows:90% ethanol solution as extraction solvent,ratio of cod roe to solvent 1:10 (g/mL),extraction time 6 h and extraction times 3.This extraction process had low cost,simple,and less pollution,and was suitable for large scale industrialization.
- cod roe /
- orthogonal experiment /
- phospholipid /
- extraction process