猴头菇多糖的乙酰化修饰及其抗氧化活性研究

徐兵; 王华丽; 林晓颖; 陈义勇

doi:10.13386/j.issn1002-0306.2018.08.010

猴头菇多糖的乙酰化修饰及其抗氧化活性研究

常熟理工学院, 生物与食品工程学院, 江苏常熟 215500

基金项目:

苏州市农业科技创新项目(SNG201615)。

详细信息

作者简介:
徐兵(1982-),男,硕士,实验师,研究方向:微生物学,E-mail:xubing@cslg.cn。

通讯作者:
陈义勇(1974-),男,博士,副教授,研究方向:食品科学与天然活性成分,E-mail:chenyyxp@126.com。

中图分类号: TS201.1
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出版历程
- 收稿日期: 2017-08-16
- 网络出版日期: 2020-11-23
- 刊出日期: 2018-04-14

Acetylation modification of polysaccharides from Hericium erinaceus and its antioxidant activity

School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China

摘要

摘要: 以猴头菇为原料,采用传统水提法提取猴头菇多糖,并制备乙酰化猴头菇多糖。以乙酰基取代度为实验指标,研究料液比(即多糖与乙酸酐的比例(g/mL))、反应时间和反应温度对HEP乙酰化修饰取代度的影响。在单因素实验的基础上,通过响应面实验设计对乙酰化实验的工艺参数进行优化,并比较修饰前后HEP的抗氧化活性。结果表明,HEP乙酰化的最佳工艺条件为:料液比为1:34 g/mL,反应时间3 h,反应温度30 ℃,在此条件下测得猴头菇多糖乙酰化的取代度为0.609,与修饰前的HEP相比,A-HEP的抗氧化性均有显著提高。乙酰化修饰是一种能够有效提高猴头菇多糖抗氧化活性的一种方法。
- 猴头菇多糖,乙酰化修饰,抗氧化活性
Abstract: Hericium erinaceus(HE)was used as raw materials. Polysaccharides from Hericium erinaceus(HEP)was extracted by water. Acetylation of polysaccharides from Hericium erinaceus(A-HEP)were prepared.The degree of substitution was used as index,the effect of the ratio of polysaccharides to acetic anhydride(g/mL),reaction temperature and time on the degree of substitution of polysaccharide was studied. Based on single factor experiment,response surface experiments with Box-Behnken design for the optimization of acetylation process were adopted. Antioxidant activity of HEP and A-HEP was discussed.The optimal conditions of A-HEP were as follows:Material liquid ratio of 1:34 (g/mL),reaction time of 3 h and reaction temperature of 30 ℃.Under the optimum process conditions,the degree of A-HEP substitution was 0.609.Compared with the unmodified HEP,the oxidation resistances of A-HEP were improved. Acetylation modification was a method that could effectively improve the antioxidant activity of HEP.
- polysaccharides from Hericium erinaceus