摘要:
为进一步提高虫草素的产量,研究了不同浓度的三十烷醇、赤霉素和6-苄基腺嘌呤以及它们的组合对蛹虫草表面液体发酵生长及虫草素积累的影响。采用中心复合实验设计和响应面方法建立数学模型优化植物生长调节的组合。结果表明,这三种植物生长调节剂对蛹虫草的生长及虫草素的积累均有不同程度地促进作用。单独使用时,赤霉素的促生对蛹虫草作用最明显,其最佳质量浓度为10 mg/L。三十烷醇促进虫草素积累的作用最明显,其最佳质量浓度为0.6 mg/L。组合使用时,0.55 mg/L三十烷醇、22.64 mg/L赤霉素与1.69 mg/L 6-苄基腺嘌呤的组合是最有利于虫草素积累的组合,虫草素最大产量达到7.31 g/L。因此,该植物生长调节剂的组合是有利于虫草素的积累。
Abstract:
To further improve the cordycepin production, the effects of three plant growth regulators, triacontanol, gibberellin and 6-benzylaminopurine, and their combinations on mycelial growth of Cordyceps militaris and cordycepin accumulation were investigated in the surface liquid fermentation. Through central composite design and response surface method, a mathematical model was developed to identify the optimal value of plant growth regulators combination for high cordycepin production. The results showed that the three plant growth regulators can promote the growth of Cordyceps militaris and cordycepin accumulation to different degrees. When the three were used respectively, gibberellin exhibited the best growth promoting effect and the optimum concentration was 10 mg/L. The triacontanol exhibited the best cordycepin-producing effect and the optimum addition was 0.6 mg/L. The optimal combination of 0.55 mg/L triacontanol, 22.64 mg/L gibberellin and 1.69 mg/L 6-benzylaminopurine was the most favorable to cordycepin accumulation. Under the optimal conditions, the maximum cordycepin yield reached 7.31 g/L.Therefore, the combination of the plant growth regulators was favourable for cordycepin accumulation
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