摘要:
目的:研究美味牛肝菌多糖对2型糖尿病(T2DM)大鼠肝损伤的改善作用。方法:采用高脂高糖饲料喂养联合链脲佐菌素(STZ)腹腔注射法构建T2DM大鼠模型。将T2DM大鼠随机分成模型组、牛肝菌多糖低、高剂量组(200,400 mg/kg·bw)、二甲双胍组(200 mg/kg·bw),以正常组作对照,正常组与模型组给予生理盐水。各组灌胃给药1次/d,4周后,测定各组大鼠空腹血糖(GLU)值并计算肝脏指数;测定血清中甘油三酯(TG)含量,谷丙转氨酶(ALT)、谷草转氨酶(AST)的活力;检测肝脏中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力,还原型谷胱甘肽(GSH)和丙二醛(MDA)含量。结果:与模型组相比,三组药物组大鼠的GLU水平显著降低(p<0.05)。TG和MDA含量减少,肝脏指数下降,ALT、AST的活力显著降低(p<0.01);CAT、SOD、GSH-Px活力和GSH的含量极显著升高(p<0.01)。结论:美味牛肝菌多糖对减轻T2DM大鼠的肝损伤有显著的疗效。
Abstract:
Objective:The objective of this work was to investigate the effect of Boletus edulis polysaccharide on the liver injury in type 2 diabetes mellitus (T2DM) rats. Methods:The rats were fed with high-fat high-sugar fodder and then injected with streptozocin (STZ) to induce T2DM rat model. Then the T2DM rats were divided randomly into model group, Bolete polysaccharide dose groups (200, 400 mg/kg·bw) and metformin group (200 mg/kg·bw), the normal rats were used as blank control, the normal and model group rats were fed with normal saline. The drugs were infused to stomach of every group of rats and once a day for 4 weeks. Then the levels of blood glucose (GLU) were detected, the liver indexs were calculated;the contents of triglyceride (TG), the activities of Glutamic-pyruvic Transaminase (ALT) and Glutamic oxalacetic Transaminase (AST) in serum were measured;the activities of catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) and the contents of glutathione (GSH), malonaldehyde (MDA) in the liver were detected. Results:Compared with the model group, three drug groups showed obviously lower contents of GLU, TG and MDA, liver index, activities of ALT and AST;and higher activities of CAT, SOD and GSH-Px, contents of GSH, the difference was significant. Conclusion:Boletus edulis polysaccharide can observably relieve the liver injury in T2DM rats.
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