摘要:
为了得到微波辅助提取人参花黄酮的最佳工艺,在单因素的基础上,以黄酮提取量为响应值,通过响应面法对其工艺进行优化。并对经大孔树脂D101纯化后的人参花黄酮进行了体外抗氧化活性研究。结果表明最佳工艺条件为:微波功率800 W,微波时间30 s,乙醇质量分数54%,料液比1 g:21 g,此时黄酮提取量为5.624 mg/g,与模型预测值接近,表明模型适用。当人参花黄酮浓度为0.24 mg/mL时,对DPPH、羟基自由基、超氧阴离子3种自由基清除能力最好,自由基清除率分别为71.47%、49.91%和72.32%,IC50分别为0.17、0.24、1.73 mg/mL,表明人参花黄酮具有较强的体外抗氧化活性,有很好的开发价值。
关键词:
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人参花
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黄酮
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响应面法
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优化
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抗氧化活性
Abstract:
To obtain the optimal microwave-assisted extraction process of total flavonoids from ginseng flower. On the basis of single-factor test,the quadratic regression parasitism model was established through the response surface methodology. The antioxidant activity was analysized after purified with D101 macroporous resin.The results indicated that the optimal extraction conditions were microwave power of 800 W,time of 30 s,alcohol concentration of 54%,material-liquid ratio of 1 g:21 g,the maximum yield of total flavonoids was 5.624 mg/g,which results fitted well with the prediction of quadratic regression parasitism model. The good antioxidant activity had been determined by scavenging effect of flavonoids from ginseng flower on 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical(71.47%),hydroxyl free radicals(49.91%)and superoxide anion free radical(72.32%)with the IC50 of 0.17,0.24,1.73 mg/mL respectively,when GFF concentration was 0.24 mg/mL. All results established a good scavenging ability to free radicals of flavonoids of ginseng flower with good development.
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