Screening of Degradable Urethane Yeast and Optimization of Its Degradation Conditions
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摘要: 本实验旨在筛选得到一株可以有效降解氨基甲酸乙酯的酵母菌,并根据ITS DNA片段鉴定其种属。采用气相色谱-质谱联用(GC-MS)法,测定所筛选株菌的发酵产物中氨基甲酸乙酯的含量,确定一株降解能力较强的菌株,根据系统发育树确定酵母种属,并对该菌株的降解条件进行了优化。结果表明,降解能力较强的菌株为G1-3,为鲁考弗梅奇(Metschnikowia reukaufii)酵母,优化降解条件为接种量为3%,38℃,170 r/min,发酵4.5 d,最终氨基甲酸乙酯的降解量在65.57%左右。
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关键词:
- 氨基甲酸乙酯 /
- 酵母菌 /
- 筛选 /
- 气相色谱-质谱联用(GC-MS) /
- 降解条件
Abstract: The purpose of this experiment was to screen a yeast that could effectively degrade urethane and identify its species by ITS DNA fragment. The content of urethane in the fermented product of the selected strains was determined by gas chromatography-mass spectrometry(GC-MS),a yeast was determined which had a strong degradation capacity,the species was identified according to the phylogenetic tree,and the degradation conditions were optimized. The results showed that,it was G1-3 which had the stronger degradation capability,identified Metschnikowia reukaufii,the optimized degradation conditions were:the amount of inoculation 3%,38℃,170 r/min,fermented for 4.5 days,the final degradation of quantity was around 65.57%.
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