Ameliorate Effect of Fractions of Schisandra chinensis Acidic Polysaccharide on Acute Alcoholic Liver Injury in Mice
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摘要: 目的:研究五味子酸性多糖分级产物(Schisandra chinensis acidic polysaccharides-2,SCAP-2)对酒精诱导小鼠急性肝损伤的保护作用。方法:采用50%乙醇12 mL/kg·bw灌胃建立小鼠急性肝损伤模型。将50只小鼠随机分为正常对照组、模型组、SCAP-2低剂量(5 mg/kg·bw)、SCAP-2中剂量组(10 mg/kg·bw)和SCAP-2高剂量组(20 mg/kg·bw)。检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)水平,超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;测定肝组织中MDA、甘油三酯(TG)含量以及SOD活性;HE染色观察肝脏组织病理学变化。结果:与酒精性肝损伤模型组相比,SCAP-2高剂量可降低小鼠血清中ALT和AST水平(p<0.05),升高血清及肝组织中SOD活性(p<0.01)、降低MDA含量(p<0.05),降低肝组织中TG含量(p<0.01),并可改善肝脏病理学变化。结论:五味子酸性多糖分级产物(SCAP-2)对小鼠急性酒精性肝损伤具有一定的保护作用。Abstract: Objective:To study the ameliorations of the fractions of Schisandra chinensis polysaccharide-2(SCAP-2)on the acute liver injury induced by alcohol in mice. Methods:An acute liver injury model was established by giving 12 mL/kg·bw 50% alcohol to mice. Fifty mice were randomly divided into normal control group,model group,and low-dose SCAP-2(5 mg/kg·bw),middle-dose SCAP-2(10 mg/kg·bw)and high-dose SCAP-2(20 mg/kg·bw)groups. The activities of lanine amino-transferase(ALT),aspartate amino-transferase(AST),and superoxide dismutase(SOD),as well as the contents of malondialdehyde(MDA)in the serum of mice were measured. MDA,triglyceride(TG)contents and SOD activities in the liver tissue were tested. HE staining was performed to observe pathological changes of the liver tissue. Results:Compared with the model group,high-dose SCAP-2 could significantly decrease the activities of ALT and AST in the serum(p<0.05),increase the SOD activities(p<0.05)and reduce the MDA contents in the serum and liver tissue(p<0.05),reduce the TG(p<0.05)content in the liver tissue(p<0.01),and improve the pathological changes of the liver. Conclusions:SCAP-2 had a protective effect on the acute alcoholic liver injury in mice.
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Keywords:
- Schisandra chinensis /
- polysaccharide /
- alcoholic liver injury /
- protective effect
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