The Anxielytic-like Activity of Enzymatic Hydrolysate of Undaria pinnatifida
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摘要: 目的:研究裙带菜酶解物的抗焦虑作用。方法:健康雄性ICR小鼠50只,随机分成5组,空白对照组、阳性对照安定(2 mg/kg)、裙带菜酶解物组(1 g/kg)、裙带菜酶解物透析液滤过液组(1 g/kg)和残留液组(1 g/kg),通过高架十字迷宫实验考察裙带菜酶解物的抗焦虑作用。利用ELISA法检测裙带菜酶解物长期灌胃后小鼠脑组织中多巴胺与5-羟色胺含量的变化。结果:裙带菜酶解物中透析滤过液的组分显著增加了小鼠在高架十字迷宫装置中进入开臂的次数及滞留时间(p<0.01),长期灌胃28 d后透析滤过液组小鼠脑组织中5-羟色胺的含量显著减少(p<0.05),多巴胺含量没有显著变化(p>0.05)。而酶解物组和残留液组进入开臂的次数和滞留时间均无显著性影响(p>0.05),同时脑组织中5-羟色胺和多巴胺的含量也无显著变化(p>0.05)。结论:裙带菜酶解物中分子量小于6 kDa的组分具有抗焦虑作用,其作用机理与脑组织中5-羟色胺含量的变化有关。Abstract: Objective:To investigate the anxielytic-like activity of enzymatic hydrolysate of Undaria pinnatifida. Methods:Fifty male ICR mice were randomly divided into five groups,ten mice per each group:the control group,Diazepam(2 mg/kg),enzymatic hydrolysate of Undaria pinnatifida(1 g/kg),enzymatic hydrolysate dialysate(1 g/kg)and enzymatic hydrolysate dialysis residual(1 g/kg). The contents of dopamine and 5-hydroxytryptamine were tested by ELISA measurement after long term oral administration. Results:Enzymatic hydrolysate dialysate group significantly increased the visits and the time in open arms(p<0.01),respectively. And Enzymatic hydrolysate dialysate group(p<0.05)reduced the content of 5-hydroxytryptamine in mice brain tissue after long term administration. However,there was no significant change on the content of dopamine(p>0.05). Moreover,enzymatic hydrolysate group and enzymatic hydrolysate dialysis group showed no significant effect on the visits and the time in open arms(p>0.05),respectively. And there were no significant changes on the content of 5-hydroxytryptamine and dopamine(p>0.05). Conclusion:Enzymatic hydrolysate of Undaria pinnatifida,of which the molecule weight was below 6 kDa,showed the anxiolytic-like activity. The mechanism might be associated with the content of 5-hydroxytryptamine in ICR mice brain tissue.
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