青海牧区产凝乳酶细菌多样性分析

朱艳; 罗俏俏; 马江; 杨敏; 文鹏程; 张忠明; 朱建宁; 贺晓玲; 张卫兵

doi:10.13386/j.issn1002-0306.2018.16.016

青海牧区产凝乳酶细菌多样性分析

1. 甘肃农业大学食品科学与工程学院, 甘肃兰州 730070;
2. 甘肃农业大学理学院, 甘肃兰州 730070;
3. 甘肃省食品药品监督管理局, 甘肃兰州 730000

基金项目:

国家自然科学基金项目(31560442,31760466)

甘肃省自然科学基金项目(1606RJZA079)

十二五"农村领域国家科技计划项目(2011AA100903)。

甘肃农业大学青年导师扶持基金项目(GAU-QNDS-201502)

甘肃农业大学伏羲人才项目(FXRC20130110)

详细信息

作者简介:
朱艳(1977-),女,博士,讲师,主要从事食品微生物方面的研究,E-mail:45330301@qq.com。

通讯作者:
张卫兵(1974-),男,博士,教授,主要从事乳品微生物和乳品加工方面的研究,E-mail:zwb126@qq.com。

中图分类号: TS252.1
计量
- 文章访问数: 141
- HTML全文浏览量: 22
- PDF下载量: 8
出版历程
- 收稿日期: 2017-10-29
- 网络出版日期: 2020-11-23
- 刊出日期: 2018-08-14

Diversity of Bacteria Producing Milk-clotting Enzymes in Qinghai Pastoral Area

1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
2. College of Science, Gansu Agricultural University, Lanzhou 730070, China;
3. Gansu Food and Drug Administration, Lanzhou 730000, China

摘要

摘要: 为发掘性能优良的产凝乳酶细菌菌株,开发新型细菌凝乳酶。本文采用酪蛋白培养基从采自青海牧区土壤样品分离筛选产凝乳酶细菌,利用菌株16S rDNA基因序列对菌株进行鉴定并分析其多样性。结果表明,36个样品中分离得到21株产凝乳酶细菌,沉淀圈与菌落直径比值为1.41~5.5;分离菌株在不同培养基上凝乳酶和蛋白水解活性有明显差异,麸皮汁培养基中凝乳酶活性为11.3~1215.6 SU/mL,蛋白水解活力为14.6~59.5U/mL;21株菌中有14株菌属芽孢杆菌属(Bacillus),是产凝乳酶细菌的优势属;多样性指数分析表明,该地区产凝乳酶细菌具有丰富多样性。
- 细菌 /
- 凝乳酶 /
- 多样性
Abstract: To excavate fine milk-clothing enzyme producing strains and develop new type of milk-clothing enzyme from bacterial sources. Microorganisms with high milk-clotting activity from soil samples collecting form Qinhai pastoral area in Tibetan Plateau of China was isolated and screened. Strains isolated were identified by the 16S rRNA sequence similarity and the diversity was analyzed by the phyologentic tree. Results showed that a total of 21 bacterial strains producing milk-clotting enzymes were isolated from 36 soil samples. The ratio of deposition zone to the colony diameter among the isolated strains in the casein plate medium ranged from 1.41 to 5.5.Media affected the milk-clotting activity and proteolysis activitiy of bacteria obviously. Milk-clotting activity among the isolated strains ranged from 11.3 to 1215.6 SU/mL in wheat bran juice medium,and proteolysis activitiy was between 14.6 and 59.5 SU/mL. Out of 21 strains,there were 14 strains found to be in the genus of Bacillus,implying that Bacillus was the most dominant genus of bacteria producing milk-clotting enzymes. The analysis indicated that the diversity of bacteria producing milk-clotting enzymes in Qinhai pastoral area was high.
- bacteria /
- milk-clotting enzymes /
- diversity