超声波辅助法提取假苹婆树叶总黄酮及其清除羟自由基能力

许建本; 苏秀芳; 莫耀芳

doi:10.13386/j.issn1002-0306.2018.23.035

超声波辅助法提取假苹婆树叶总黄酮及其清除羟自由基能力

许建本^1,2,
苏秀芳^1,2, ,,
莫耀芳¹

1. 广西民族师范学院化学化工学院, 广西崇左 532200;
2. 广西高校桂西南特色植物资源化学重点实验室培育基地, 广西崇左 532200

基金项目:

桂教人〔2011〕40号2011年度"广西高校优秀人才资助计划"。

详细信息

作者简介:
许建本(1990-),男,硕士研究生,研究方向:天然产物化学,E-mail:xujianben2014@163.com。

通讯作者:
苏秀芳(1971-),女,硕士,教授,研究方向:天然产物活性成分的提取、分离、分析及药理活性研究,E-mail:suxiufang88@sina.com。

中图分类号: TS201.3
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出版历程
- 收稿日期: 2017-12-03
- 网络出版日期: 2020-11-22
- 刊出日期: 2018-11-30

Ultrasonic-assisted Extraction of Total Flavonoids from Sterculia Lanceolata and Scavenging Capacity on Hydroxyl Free Radical

1. College of Chemistry and Chemical Engineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China;
2. Guangxi Colleges and Universities Key Laboratory Breeding Base of Chemistry of Guangxi Southwest Plant Resources, Chongzuo 532200, China

摘要

摘要: 利用超声波辅助提取技术研究假苹婆树叶总黄酮的提取工艺及其抗氧化活性。以总黄酮得率为研究对象,考察了料液比,乙醇体积分数、提取功率、提取时间及提取温度对提取效果的影响,通过正交试验对提取工艺进行了优化。结果表明,最佳提取工艺条件为:乙醇体积分数为60%,提取功率为60 W,料液比为1:25(g/mL),70℃提取65 min,该条件下总黄酮得率为2.36%±0.0002%。抗氧化试验表明,假苹婆提取液对羟自由基的清除能力随着提取液中总黄酮浓度的增大而增强,当假苹婆树叶总黄酮的质量浓度为4.92 mg/mL时,清除率达到73.58%。
- 假苹婆 /
- 总黄酮 /
- 提取 /
- 抗氧化
Abstract: The ultrasonic-assisted extraction of total flavonoids from Sterculia lanceolata and antioxidant activity were studied. The influences of the ratio of Sterculia lanceolata to solvent,ethanol concentration,extraction power,extraction time and extraction temperature were investigated by single factor experiment,and the extraction process were optimized by orthogonal test with total flavonoids yield as index. The results showed that the optimal of extraction were as follows:the ratio of sterculia lanceolata to solvent was 1:25 (g/mL),ethanol concentration was 60%,extraction power was 60 W,the extraction was token place at 70℃ for 65 min. Under these conditions,the yield of total flavonoids was 2.36%±0.0002%. Antioxidant test showed that the hydroxyl radical scavenging activity increased with the increasing concentration of total flavonoids from the leaves of sterculia lanceolata. At the concentration of 4.92 mg/mL,the total flavonoids exhibited a maximum hydroxyl radical scavenging rate of 73.58%.
- Sterculia lanceolata /
- total flavonoids /
- extraction /
- antioxidation