Extraction of Astaxanthin by Switchable Hydrophilic Solvents and Antioxidant Activity of Astaranthin Nanoparticles
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摘要: 采用单因素以及正交试验优化了可切换亲水溶剂N-N二甲基环己胺(DMCHA)从雨生红球藻中提取虾青素的工艺,并将虾青素使用酰化羧甲基壳聚糖自聚体进行纳米包埋,探究虾青素纳米自聚体的抗氧化性。结果表明,在提取温度35℃,提取时间24 h,提取料液比1:20 g/mL条件下,虾青素提取率最高,达到氯仿甲醇提取的总虾青素含量的85.48%,虾青素纳米自聚体的虾青素载量可达到392 μg/mL。抗氧化性实验证明,在不同pH变化下,虾青素纳米自聚体比虾青素溶液更加稳定;55℃放置24 h条件下,虾青素纳米自聚体羟基自由基清除率仍可保持在40%,证明虾青素纳米自聚体可以有效维持虾青素的氧化稳定。Abstract: Single-factor experiments and orthogonal experiments were used to optimize the process for extracting astaxanthin from H. pluvialis using switchable hydrophilic solvent DMCHA. Acylated carboxymethyl chitosan self-polymers was used to produce astaxanthin nano-capsules. And the antioxidant properties of astaxanthin nano-capsules were explored. The results showed that at the extraction temperature of 35℃,H. pluvialis/DMCHA ratio 1:20 and extracted for 24 h,the extraction rate of astaxanthin was the highest,reaching 85.48% with the total astaxanthin content extracted by Folch method. The astaxanthin load could reach 392 μg/mL. It has been proved by antioxidant experiments that astaxanthin nano-self-polymer was more stable than astaxanthin solution under different pH changes. In addition,astaxanthin nano-self-polymer hydroxyl radicals were stored at 55℃ for 24 h. The hydroxyl radical scavenging rate could still be maintained at 40%,which proved that astaxanthin nano-self-polymer can effectively maintain the oxidation stability of astaxanthin.
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Keywords:
- astaxanthin /
- switchable hydrophilic solvent /
- chitosan /
- nano-self-polymer /
- antioxidant activity
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