摘要:
目的:探讨东北刺人参根水提取物冻干粉的抗疲劳和改善睡眠节律的作用。方法:在小鼠力竭游泳实验中,将小鼠随机分为空白对照组、东北刺人参低剂量组(125 mg/kg)、中剂量组(250 mg/kg)、高剂量组(500 mg/kg)连续灌胃给药15 d,检测血清中尿素氮(BUN)和乳酸脱氢酶(LDH)水平,以及肝组织中谷胱甘肽过氧化物酶(GSH-Px)和三磷酸腺苷酶(ATPase)活力等指标。同时采用果蝇活动监测系统(DAMS),以果蝇为观察对象,以睡眠时间和睡眠-觉醒节律为考察指标,分别考察东北刺人参对果蝇睡眠的干预作用。结果:东北刺人参能延长小鼠负重游泳时间,高中低剂量组延长率分别为126.16%、102.44%和62.32%,亦可提高自由泳小鼠GSH-Px、LDH和ATPase活性,降低BUN水平。对果蝇睡眠节律影响实验结果显示,东北刺人参可以增加雄果蝇平均睡眠时间,减少自主活动次数,且呈现剂量依赖性。同时,减少雌果蝇自主活动次数,缩短白天活动时间,缩短片段化睡眠,增加夜晚睡眠时间,且明显后移睡眠相位角。结论:东北刺人参能显著增加果蝇的睡眠时间,改善睡眠节律,可加速疲劳的消除,并可以延长小鼠力竭游泳时间,提高其运动耐力,减少自由基的堆积,加快体内代谢产物的清除,延缓疲劳的产生。
关键词:
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东北刺人参
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抗疲劳
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果蝇
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睡眠节律
Abstract:
Objective:To explore the anti-fatigue and sleep rhythm improvement effects of Oplopanax elatus Nakai root and rhizome extracts. Methods:Mice were randomly divided into blank control group,low dose group(125 mg/kg),middle dose group(250 mg/kg),high dose group(500 mg/kg)for 15 days in exhaustive swimming experiment. The levels of urea nitrogen(BUN)and lactate dehydrogenase(LDH)in serum and the activities of glutathione peroxidase(GSH-Px)and adenosine triphosphate(ATPase)in liver tissue were measured. At the same time,Drosophila melanogaster was observed and the sleeping time and sleep-wakefulness rhythm were taken as the indicators respectively. The intervention effect of the extract of Oplopanax elatus Nakai on Drosophila melanogaster sleep was investigated by Drosophila activity monitoring system(DAMS).Results:Oplopanax elatus Nakai.could extend the weight-bearing swimming time of mice,the extension rates of high,medium and low dose groups were 126.16%,102.44% and 62.32% respectively. And also the activity of GSH-Px,LDH and ATPase in free swimming mice increased,and BUN levels reduced. The results of the experiment on the effect of Drosophila sleep rhythm showed that Oplopanax elatus Nakai.could increase the sleep time of male Drosophila and reduce the number of autonomous activities in a dose-dependent manner. At the same time,the impact on sleep of females was manifested as reducing the number of spontaneous activities,shortening the activity time of the day,shortening the fragmented sleep,increasing the night's sleep time,and obviously shifting the sleep phase angle. Conclusion:Oplopanax elatus Nakai. can dramatically enhance the sleep time of Drosophila and improve sleep rhythm,and it can extend the exhaustive swimming time of mice,improve their exercise endurance,reduce the accumulation of free radicals,accelerate the elimination of metabolites in the body,improve sleep and exertanti-fatigue effects.
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