摘要:
目的:确定甘肃陇南大红袍花椒芳香油的化学成分及对于大肠杆菌、金黄色葡萄球菌、破伤风杆菌、荧光假单胞杆菌四种细菌的抑菌效果。方法:采用水蒸气蒸馏法提取陇南大红袍花椒芳香油,运用气相色谱-质谱联用(GC-MS)对其成分进行分析鉴定,并研究了陇南大红袍花椒芳香油的抑菌活性。结果:陇南大红袍花椒芳香油鉴定出50种化学成分,占总量的(92.49%),主要的化学成分包括D-柠檬烯(32.08%)、右旋香芹酮(20.02%)、石竹烯(12.26%)等;通过抑菌实验发现陇南大红袍花椒芳香油对于4种细菌均具有抑菌活性,其中对金黄色葡萄杆菌和荧光假单胞杆菌抑菌效果较好,最低抑菌浓度(MIC)和最低杀菌浓度(MBC)分别为2.5和5.0 mL/L、2.5和5.0 mL/L。结论:本文确定了甘肃省陇南大红袍花椒芳香油的化学成分,并得出了陇南大红袍花椒挥发油对于大肠杆菌、金黄色葡萄球菌、破伤风杆菌、荧光假单胞杆菌的MIC和MBC,为花椒的进一步应用奠定一定的理论基础。
Abstract:
Objective:The purpose of this experiment was to determine the chemical constituents of Zanthoxylum bungeanum aromatic oil from Longnan Dahongpao,Gansu Province,and its bacteriostatic effect on four bacteria:Escherichia coli,Staphylococcus aureus,Nicolaieri's bacillus and Pseudomonas fluorescens. Methods:The aromatic oil from Zanthoxylum bungeanum L. in Longnan Dahongpao was extracted by steam distillation. The components were analyzed and identified by gas chromatography-mass spectrometry(GC-MS). The antimicrobial activity of the aromatic oil from Zanthoxylum bungeanum L. in Longnan Dahongpao was studied. Results:An amount of 50 chemical constituents were identified in the aromatic oils of Zanthoxylum bungeanum in Dahongpao,Longnan,accounting for 92.49% of the total. The main chemical constituents included D-limonene(32.08%),dextrocarvone(20.02%)and a-humulene(12.26%). The results showed that the aromatic oil of Zanthoxylum bungeanum had bacteriostatic activity against four kinds of bacteria.Among them,the antibacterial effect of Staphylococcus aureus and Pseudomonas fluorescens was better. The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were 2.5 and 5.0 mL/L,respectively. Conclusion:The chemical constituents of the aromatic oil of Zanthoxylum bungeanum from Longnan Dahongpao in Gansu Province were determined,and the minimum inhibitory concentration and bactericidal concentration of the essential oil of Zanthoxylum bungeanum for E.coli,S. aureus,N. bacillus and P. fluorescens were obtained,which laid a theoretical foundation for the further application of Zanthoxylum bungeanum.
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