Fe<sub>3</sub>O<sub>4</sub>磁性纳米粒子固定化微泡菌褐藻胶裂解酶AlgL17的研究

张曦文; 宋昕祺; 李鹤宾; 姜泽东; 倪辉; 朱艳冰

doi:10.13386/j.issn1002-0306.2020.06.004

Fe₃O₄磁性纳米粒子固定化微泡菌褐藻胶裂解酶AlgL17的研究

张曦文¹,
宋昕祺¹,
李鹤宾²,
姜泽东^1,3,4,5,
倪辉^1,3,4,5,
朱艳冰^1,3,4,5, ,

1. 集美大学食品与生物工程学院, 福建厦门 361021;
2. 厦门医学院药学系, 福建厦门 361023;
3. 福建省食品微生物与酶工程重点实验室, 福建厦门 361021;
4. 厦门市食品生物工程技术研究中心, 福建厦门 361021;
5. 厦门南方海洋研究中心经济海藻资源化利用与深加工重点实验室, 福建厦门 361021

基金项目:

国家自然科学基金(31401632)。

详细信息

作者简介:
张曦文(1996-),女,硕士研究生,研究方向:食品酶学,E-mail:AdelaZhang96@163.com。

通讯作者:
朱艳冰(1976-),女,博士,教授,研究方向:食品酶学方向,E-mail:yanbingzhu@163.com。

中图分类号: TS201.2
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出版历程
- 收稿日期: 2019-06-03
- 网络出版日期: 2020-11-12
- 刊出日期: 2020-03-14

Immobilization of Alginate Lyase AlgL17 from Microbulbifer sp. ALW1 with Fe₃O₄ Magnetic Nanoparticles

ZHANG Xi-wen¹,
SONG Xin-qi¹,
Li He-bin²,
JIANG Ze-dong^1,3,4,5,
NI Hui^1,3,4,5,
ZHU Yan-bing^1,3,4,5, ,

1. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China;
2. Department of Pharmacy, Xiamen Medical College, Xiamen 361023, China;
3. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China;
4. Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China;
5. Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed, Xiamen Southern Ocean Technology Center of China, Xiamen 361021, China

摘要

摘要: 本论文旨在优化微泡菌褐藻胶裂解酶AlgL17固定化条件,以期探索出高效的固定化条件。以单宁酸功能化的四氧化三铁磁性纳米粒子作为载体,对褐藻胶裂解酶AlgL17进行固定化条件优化。测定固定化酶的最适反应温度和最适反应pH,并对固定化酶进行傅里叶变换红外光谱(FTIR)和扫描电镜(SEM)检测。结果表明,褐藻胶裂解酶AlgL17最佳固定化条件为:加酶量2.7 U、载体量25 mg、固定时间5 h,固定化酶的最适反应条件为:温度35℃、pH8.5。FTIR和SEM结果显示,褐藻胶裂解酶AlgL17固定在载体表面。四氧化三铁磁性纳米粒子可以成功固定褐藻胶裂解酶AlgL17,固定化后的褐藻胶裂解酶具有工业应用的前景。
- 磁性纳米粒子 /
- 褐藻胶裂解酶 /
- 固定化酶 /
- 条件优化
Abstract: This study aims to optimize the immobilization conditions of alginate lyase AlgL17 from Microbulbifer sp.,exploring the efficient conditions. By using tannic acid functionalized Fe₃O₄ magnetic nanoparticles as carriers,optimization of the immobilization conditions of AlgL17 was carried out. The optimum reaction temperature and pH of the immobilized enzyme were determined,and then the immobilized enzyme was analyzed by using scanning electron microscope(SEM)and Fourier transform infrared spectroscopy(FTIR). The results showed that the optimized immobilization conditions for AlgL17 were enzyme amount of 2.7 U,carrier amount of 25 mg,immobilization time of 5 h. Optimal reaction conditions for immobilized enzyme were of 35℃ and pH8.5.SEM and FTIR analysis showed that alginate lyase AlgL17 was immobilized on the surface of the carrier. Alginate lyase AlgL17 was successfully immobilized on Fe₃O₄ magnetic nanoparticles. The immobilized alginate lyase AlgL17 has potential in the industrial applications.
- magnetic nanoparticles /
- alginate lyase /
- immobilized enzyme /
- optimization