摘要:
以黄精总皂苷得率为评价指标,通过单因素试验对纤维素酶添加量、果胶酶添加量、料液比、酶解pH、酶解温度以及酶解时间进行研究,采用响应面对提取条件进行优化,并以阿卡波糖为阳性对照,探究不同浓度下黄精总皂苷的α-淀粉酶及α-葡萄糖苷酶抑制活性。结果表明,最佳提取条件为:纤维素酶添加量0.4%、果胶酶添加量5.0%、料液比1:16 g/mL、酶解pH为5.0、酶解温度45℃、酶解时间2.0 h,总皂苷得率4.06%。当黄精总皂苷浓度为3.000 mg/mL时,其对α-葡萄糖苷酶最高抑制率可达74%,接近于阿卡波糖(0.5 mg/mL)的82%;当黄精总皂苷浓度为2.000 mg/mL时,其对α-淀粉酶最高抑制率可达82%,超过阿卡波糖(0.5 mg/mL)的80%。本研究使用的复合酶法提高了黄精总皂苷得率并证实了其具有一定的α-葡萄糖苷酶和α-淀粉酶抑制活性。
Abstract:
Taking the yield of Polygonatum sibiricum saponins as the evaluation index,the cellulase addition amount,pectinase addition amount,material-liquid ratio,enzymatic pH,enzymatic temperature,and enzymatic hydrolysis time were studied by single factor tests. The extraction conditions were optimized,and acarbose was used as a positive control to investigate the inhibitory activities of saponins from Polygonatum sibiricum at different concentrations on α-amylase and α-glucosidase. The results showed that the optimal extraction conditions were:Cellulase addition amount of 0.4%,pectinase addition amount of 5.0%,material-liquid ratio of 1:16 g/mL,enzymolysis pH5.0,enzymolysis temperature 45℃,and enzymolysis time at 2.0 h,the yield of total saponins was 4.06%. When the concentration of Polygonatum sibiricum saponin was 3.000 mg/mL,the highest inhibition rate of α-glucosidase was 74%,which was close to 82% of acarbose(0.5 mg/mL). When the concentration of Polygonatum sibiricum saponin was at 2.000 mg/mL,the highest inhibition rate of α-amylase could reach 82%,exceeding 80% of acarbose(0.5 mg/mL).The compound enzyme method used in this study improved the yield of Polygonatum sibiricum saponins and confirmed that it had certain α-glucosidase and α-amylase inhibitory activities.
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