Protective Effect and Mechanism Study of Jujube Polysaccharides on Intestinal Immune Barrier in Mice
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摘要: 目的：研究红枣多糖（Jujube Polysaccharides，JPS）对小鼠肠道免疫屏障的保护作用及其机制。方法：将70只雄性ICR小鼠随机分为5组：阴性对照组、模型组、JPS低（150 mg/kg·bw）、中（300 mg/kg·bw）、高（600 mg/kg·bw）剂量组，连续灌胃28 d，第28 d采用腹腔注射环磷酰胺造模。研究JPS对免疫抑制小鼠免疫器官指数、肠道派伊尔结数量、肠道免疫相关指标、肠道免疫细胞数量和肠道免疫相关蛋白表达的影响。结果：JPS能够保护小鼠肠黏膜的正常形态结构，增加小鼠免疫器官指数和派伊尔结数量，增加肠道分泌的免疫球蛋白A和分泌型免疫球蛋白A的含量，增强肠道溶菌酶和酸性磷酸酯酶的活性，增加肠道免疫细胞的数量，下调肠道MyD88、NF-κB p65、IL-1β蛋白表达。结论：JPS对小鼠的肠道免疫屏障具有保护作用，其机制与调节MyD88/NF-κB途径相关蛋白表达有关。Abstract: Objective:To study the protective effect of jujube polysaccharides extract on intestinal immune barrier and its mechanism in mice. Methods:70 male ICR mice were randomly divided into 5 groups:negative control group,model group,low(150 mg/kg·bW),medium(300 mg/kg·bW),and high(600 mg/kg·bW)dose group. The mice were gavage continuously for 28 d,and the model was established by intraperitioneal injection of cyclophosphamide on the last day. To study the effects of JPS on the immune organ index,the number of intestinal Peyer’s patches,the intestinal immune-related indicators,the number of intestinal immune cells and the expression of intestinal immune-related proteins in immunosuppressed mice.Results:JPS could protect the normal morphological structure of the intestinal mucosa of mice,increase the index of immune organs and the number of Peyer’s knots in mice,increase the content of IgA and SIgA secreted by the intestine,and enhance intestinal lysozyme and acid phosphatase activity,increases the number of intestinal immune cells and down-regulates the expression of MyD88,NF-κB p65,and IL-1β proteins. Conclusion:Jujube polysaccharide extract has a protective effect on the intestinal immune barrier of immunosuppressed mice,and its mechanism is related to the regulation of MyD88/NF-B pathway related protein expression.
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Keywords:
- jujube polysaccharide /
- intestinal barrier /
- immune barrier /
- protective effect /
- mechanism research
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[1] Gao Q H,Wu C S,Wang M.The jujube(Ziziphus jujuba mill.)fruit:A review of current knowledge of fruit composition and health benefits[J]. Journal of Agricultural and Food Chemistry,2013,61(14):3351-3363.
[2] 中华人民共和国国家统计局网[EB/OL].(2018)[2020-6-9]. http://data.stats.gov.cn/adv.htm?m=advquery&cn=C01. [3] Li J W,Shan L,Liu Y F,et al. Screening of a functional polysaccharide from Zizyphus jujuba cv.Jinsixiaozao and its property[J]. International Journal of Biological Macromolecules,2011,49(3):255-259.
[4] 王富刚,魏永义.红枣汁降血脂营养功能性研究[J]. 食品研究与开发,2017,38(17):183-185. [5] 潘莹,许经伟.冬枣多糖的分离纯化及抗氧化活性研究[J]. 食品科学,2016,37(13):89-94. [7] Cosmulescu S,Trandafir I,Nour V,et al. Variation of bioactive compounds and antioxidant activity of jujube(Ziziphus jujuba)fruits at different stages of ripening[J]. Notulae Botanicae Horti Agrobotanici Cluj-Napoca,2018,46(1):134-137.
[8] 黄艳芬,刘湘红,伍浩,等.肠黏膜屏障与肠道菌群的相互关系[J]. 中国微生态学杂志,2019,31(12):1465-1469 ,1474.
[9] 李永渝.肠道屏障功能障碍的病理生理机制[J]. 胃肠病学,2006,11(10):629-632. [10] Wang D Y,Zhao Y,Jiao Y D,et al. Antioxidative and hepatoprotective effects of the polysaccharides from Zizyphus jujube cv.Shaanbeitanzao[J]. Carbohydrate Polymers,2012,88(4):1453-1459.
[11] Liu G P,Liu X Q,Zhang Y C,et al. Hepatoprotective effects of polysaccharides extracted from Zizyphus jujube cv.Huanghetanzao[J]. International Journal of Biological Macromolecules,2015,76:169-175.
[12] Li,Hong,Zhang,et al. Immunomodulating and antioxidant effects of polysaccharide conjugates from the fruits of Ziziphus jujube on chronic fatigue syndrome rats[J]. Carbohydrate Polymers Scientific & Technological Aspects of Industrially Important Polysaccharides,2015:189-196.
[13] 李学敏,于卿,郑荣,等.4种常见海参对小鼠肠道黏膜免疫功能的保护作用[J]. 食品科学,2013,34(15):240-245. [14] 唐天龙.巴楚蘑菇多糖成分对小鼠抗氧化以及免疫功能的影响[J]. 中国食用菌,2019,38(10):39-42. [15] 郜艳雪,时坤,李健明,等.党参多糖对动物免疫调节作用研究进展[J]. 动物医学进展,2019,40(9):103-106. [16] 陈炅然,胡庭俊,张瑞孝,等.蕨麻多糖对小鼠免疫功能的影响[J]. 中兽医医药杂志,2005,24(2):14-16. [17] 邱培勇,徐自超,陈正跃,等.黄芪多糖对小鼠免疫功能的影响[J]. 新乡医学院学报,2006,23(6):585-586. [18] 高娟,李芳兰,周一珺,等.不同发育时期大鼠小肠粘膜及派氏结T淋巴细胞发育活化的研究[J]. 中国免疫学杂志,2009,25(8):678-683. [19] Galdeano C M,Perdigón G.The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity[J]. Clinical and Vaccine Immunology,2006,13(2):219-226.
[20] 周华,王培训,刘良,等.环磷酰胺对小鼠Peyer's结和肠道粘膜相关淋巴细胞的影响[J]. 中国免疫学杂志,2001,17(4):186-187 ,189,170.
[21] 郭佳裕,孟娟,杜锦辉.甘草泻心汤对抗生素诱导肠道菌群失调小鼠肠道主要菌群及sIgA的影响[J]. 中国微生态学杂志,2019,31(11):1246-1249 ,1259.
[22] Corthésy B.Role of secretory IgA in infection and maintenance of homeostasis[J]. Autoimmunity Reviews,2013,12(6):661-665.
[23] Salerno-Goncalves R,Safavie F,Fasano A,et al. Free and complexed-secretory immunoglobulin A triggers distinct intestinal epithelial cell responses[J]. Clinical & Experimental Immunology,2016,185(3):338-347.
[24] 韩渤,刘玥宏,仇志强,等.SIgA表达功能及其在肠道疾病的作用[J]. 世界华人消化杂志,2017,25(19):1757-1763. [25] 杨雅静,裴银辉,李琳.芒柄花黄素对实验小鼠免疫功能的影响[J]. 安徽农业科学,2019,47(10):86-88 ,100.
[26] 王佃亮.重组人溶菌酶研究进展[J]. 中国生物工程杂志,2003,23(9):59-63. [27] 金政,王启伟,李相伍,等.肝损伤后溶酶体和酸性磷酸酶活性改变和蓝靛果的作用[J]. 延边大学医学学报,2001,24(2):79-81. [28] 黄秀芳,朱义红,曹汴川,等.利福喷丁对大鼠IFN-γ、IL-12、溶菌酶及酸性磷酸酶活性的影响[J]. 中国抗生素杂志,2015,40(6):464-467. [29] 张大鹏,周联,张志敏,等.四君子汤复方总多糖对小鼠肠上皮间淋巴细胞的免疫调节作用[J]. 中药新药与临床药理,2009,20(3):204-206. [30] 廖吕燕. "芪苓"制剂多糖对免疫抑制小鼠肠道黏膜免疫及免疫调节作用的影响[D]. 福州:福建农林大学,2010. [31] 陈玲珑,郑鸣,刘梅珍,等.阻断交感神经对小鼠小肠黏膜免疫相关细胞的影响[J]. 解剖科学进展,2008,14(3):305-308 ,311.
[32] 胡艳艳,刘小伟.肠道杯状细胞结构和功能的研究进展[J]. 国际病理科学与临床杂志,2013,33(5):424-430. [33] 黄玉章. 黄芪多糖对奥尼罗非鱼生长性能和免疫功能的影响[D]. 福州:福建农林大学,2009. [34] 张玉仙,王文利,陈耀星,等.不同浓度大蒜溶液对小鼠小肠黏膜免疫相关细胞数量变化的影响[J]. 中国兽医杂志,2009,45(4):32-34. [35] Takeda K,Akira S.TLR signaling pathways[J]. Seminars in Immunology,2004,16(1):3-9.
[36] Shifera A S.The zinc finger domain of IKKγ(NEMO)protein in health and disease[J]. Journal of Cellular and Molecular Medicine,2010,14(10):2404-2414.
[37] 朱磊,沈洪,顾培青,等.黄芩苷对溃疡性结肠炎模型大鼠NF-κB表达的影响[J]. 南京中医药大学学报,2016,32(5):447-450. [38] Shen Y,Zhong J H,Xu L,et al. Effects of berberine hydrochloride on the expression of TNF-α,IL-1βand IL-10 of colon tissue in mice with ulcerative colitis[J]. Chin J Mod Appl Pharm(中国现代应用药学),2017(8):1094-1098.
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