Influence of oxidation on the nature of liposome membrane
More Information
-
-
- Received Date:
September 17, 2012
-
-
Abstract
The subject prepared liposomes with soybean phosphatidylcholine (PC) and used the liposome as bilayer membrane structure model to understand the effect of oxidation on membrane.Fenton reaction was used to control the oxidation of liposome, and its properties including MDA, particle size, Zeta, appearance, membrane polarity, entrapment efficiency and membrane liquidity was measured.The degree of oxidation could be controlled by the reaction time of Fenton, with the Fenton reagent adding, the particle size of the liposomes first became larger and then smaller, and its distribution was more uneven.The ζ-potential, entrapment efficiency and membrane liquidity declined.In addition, the polarity of membrane increased slightly.
-
-
References
| [1] |
Malam Y, Loizidou M, Seifalian A M, et al.Liposomes and nanoparticles:nanosized vehicles for drug delivery in cancer[J].Trends in Pharmacological Science, 2009, 30:592-599.
|
| [2] |
Borst J W, Visser N V, Kouptsova O, et al.Oxidation of unsaturated phospholipids in membrane bilayer mixtures is accompanied by membrane fluidity changes[J].Biochimica et Biophysica Acta, 2000, 1:61-73.
|
| [3] |
Megli F M, Russo L, Sabatini K, et al.Oxidized phospholipids induce phase separation in lipid vesicles[J].FEBS Letters, 2005, 579:4577-4584.
|
| [4] |
Megli F M, Sabatini K.EPR studies of phospholipid bilayers after lipoperoxidation1.Inner molecular order and fluidity gradient[J].Chemistry and Physics of Lipids, 2003, 125:161-172.
|
| [5] |
Cwiklik L, Jungwirth P.Massive oxidation of phospholipid membranes leads to pore creation and bilayer disintegration[J].Chemical Physics Letters, 2010, 486:99-103.
|
| [6] |
Soobrattee M A, Neergheen V S, Ramma A L, et al.Kinetics and mechanisms of antioxidant activity using the DPPH free radical method[J].Food Science and Technology, 1997, 30:609-615.
|
| [7] |
Min B, Nam K C, Ahn D U, et al.Catalytic mechanisms of metmyoglobin on the oxidation of lipids in phospholipid liposome model system[J].Food Chemistry, 2010, 123:231-236.
|
| [8] |
Mohsen M, Mady, Mirhane M, et al.Biophysical studies on chitosan-coated liposomes[J].European Biophysics Journal, 2009, 38:1127-1133.
|
| [9] |
Hatzi P, Mourtas S, Pavlos G, et al.Integrity of liposomes in presence of cyclodextrins:Effect of liposome type and lipid composition[J].International Journal of Pharmaceutics, 2007, 333:167-176.
|
| [10] |
Wrobel D, Ionov M, Gardikis K, et al.Interactions of phosphorus-containing dendrimers with liposomes[J].Biochimica et Biophysica Acta, 2011, 3:221-226.
|
| [11] |
王振华, 王玉蓉.脂质体表面电荷的研究进展[C].全国中药创新与研究论坛, 2009.
|
| [12] |
邓英杰.脂质体技术[M].北京:人民卫生出版社, 2007:242-245.
|
-
DownLoad: