LI Jia-nan, WANG Jing, ZHANG Hui-juan, LI Ying-ying, LIU Ying-li, PANG Xiao-yi, JIANG Guo-qing. Enzymatic preparation of antioxidant peptides from rice protein and identification of antioxidant peptides[J]. Science and Technology of Food Industry, 2013, (23): 53-57. DOI: 10.13386/j.issn1002-0306.2013.23.080
Citation: LI Jia-nan, WANG Jing, ZHANG Hui-juan, LI Ying-ying, LIU Ying-li, PANG Xiao-yi, JIANG Guo-qing. Enzymatic preparation of antioxidant peptides from rice protein and identification of antioxidant peptides[J]. Science and Technology of Food Industry, 2013, (23): 53-57. DOI: 10.13386/j.issn1002-0306.2013.23.080

Enzymatic preparation of antioxidant peptides from rice protein and identification of antioxidant peptides

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  • Received Date: May 14, 2013
  • This antioxidant peptides prepared from rice protein hydrolyzed by Alcalase were characterized for their antioxidant properties in vitro reflected by the abilities to scavenge hydrogen peroxide as well as hydroxyl and DPPH free radicals, to chelate iron ions, and to inhibit autooxidation of linoleic acid.The antioxidant peptides were identified to be Met-Pro-Pro-Ser-Ser- Pro- His (376.68u) , Leu- Ala- Gly- Pro- Lys- Phe- Ala- Leu (408.75u) , and Met-Pro-Arg-His-Asp-Pro-Gln (440.70u) by High Definition Mass Spectrometry (HD-MS) .
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  • [1]
    Zhang J H, Zhang H, Wang L, et al.Isolation and identification of antioxidative peptides from rice endosperm protein enzymatic hydrolysate by consecutive chromatography and MALDI-TOF/TOF MS/MS[J].Food Chemistry, 2010, 119:226-234.
    [2]
    夏光华, 申铉日, 蔡锦红, 等.三酶法制备罗非鱼鱼皮胶原蛋白抗氧化肽及活性研究[J].食品科学, 2012, 33 (23) :175-179.
    [3]
    张君慧, 张晖, 王兴国, 等.大米蛋白酶解制备抗氧化肽的研究[J].食品与发酵工业, 2008, 34 (5) :71-75.
    [4]
    刘振家, 朱科学, 周惠明.超声波辅助酶解脱脂小麦胚芽制备抗氧化肽的研究[J].中国油脂, 2009, 34 (5) :38-41.
    [5]
    林松毅, 郭洋, 王莹, 等.蛋清抗氧化肽增效剂的优化[J].华南理工大学学报:自然科学版, 2010, 38 (8) :100-104.
    [6]
    邓敏, 朱志伟, 欧善堂, 等.利用响应面法优化酶解罗非鱼制备抗氧化肽的研究[J].现代食品科技, 2011, 27 (10) :1242-1245.
    [7]
    阎欲晓, 粟桂娇, 李小梅, 等.文蛤蛋白抗氧化活性肽的研究[J].食品工业科技, 2007, 28 (12) :121-123.
    [8]
    Joseph C.Correlations between the Physicochemical and Functional Properties of Rice[J].Journal of Agricultural and Food Chemistry, 1992, 40:1683-1686.
    [9]
    张燕平.味精厂下脚料米渣的开发利用[J].杭州食品科技, 1994 (3) :22-26.
    [10]
    Zhang H-J, Zhang H, Wang L, et al.Preparation and functional properties of rice bran proteins from heat-stabilized defatted rice bran[J].Food Research International, 2011, 8:1-5.
    [11]
    许明峰, 沈莲清, 王奎武, 等.雷丸多糖的提取分离及其抗氧化活性研究[J].中国食品学报, 2011, 11 (6) :42-46.
    [12]
    施瑛, 汪梅, 徐娟, 等.麦苗汁的营养成分及其抗氧化活性测定[J].食品科学, 2005, 26 (1) :215-218.
    [13]
    Mitsuda H, Yuasumoto K, Iwami K.Antioxidation action of indole compounds during the antoixdation of linoleic acid.Eiyo to Shokuryo[J], 1996, 19:210-214.
    [14]
    Yamaguchi F, Ariga T, Yoshimura Y, et al.Antioxidative and anti-glycation activity of garcinol from Garcinia indica fruit rind[J].J Agric Food Chem, 2000, 48 (2) :180-285.
    [15]
    Ruch K J, Cheng S J, Klauning J E.Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechin isolated from Chinese greenter[J].Carcinogensis, 1989, 10 (6) :1003-1008.
    [16]
    Halliwell B, Gutteridge J M C, Aruoma O I.The deoxyribose method:A simple test-tube asssy for determination of9 rate constants for reactions of hydroxyl radicals[J].Anal Chem, 1987, 165:215-219.
    [17]
    刘锋, 朱晓丹, 王鸿丽, 等.应用HD-MS分析胆结石与胆道铸型间的差异蛋白质[J].军医进修学院学报, 2010, 31 (1) :69-71.
    [18]
    岳楠.两种氨基酸添加下酪蛋白类蛋白物的抗氧化活性研究[D].哈尔滨:东北农业大学, 2012.
    [19]
    Saiga A, Tanabe S, Nishimura T.Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment[J].J Agri Food Chem, 2003, 51:3661-3667
    [20]
    Ranathunga S, Rajapakse N, Kim SK.Purification and characterization of antioxidative peptide derived from muscle of conger eel (Conger myriaster) [J].Eur Food Res Tech, 2006, 222:310-315
    [21]
    Xie Z J, Huang J R, Xu X M, et al.Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate[J].Food Chemistry, 2008, 111:370-376.
    [22]
    张君慧.大米蛋白抗氧化肽的制备、分离纯化和结构鉴定[D].无锡:江南大学, 2009.
    [23]
    李鸿梅.玉米功能肽的制备及其生理活性的研究[D].长春:吉林大学, 2008.
    [24]
    谢正军.苜蓿叶蛋白和酶解物制备及其抗氧化肽的研究[D].无锡:江南大学, 2009.
    [25]
    王灼维, 贾伟, Swee Lee Yap.SYNAPT G2 HDMS高清质谱性能特点及应用简介[J].现代仪器, 2010 (5) :20-24.
    [26]
    Park PJ, Jung WK, Kim SK.Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin-free egg yolk[J].J Am Oil Chem Soc, 2001, 78 (6) :651-656.
    [27]
    Ren JY, Zhao MM, Shi J, et al.Purification and identification of antioxidant peptides from grass carp muscle hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry[J].Food Chem, 2008, 108:727-736.

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