HUANG Zhi-li, HUANG Yan-jun, ZHANG Li-jun. Study on purification and characterization of superoxide dismutase in Trametes versicolor mycelium[J]. Science and Technology of Food Industry, 2014, (02): 120-123. DOI: 10.13386/j.issn1002-0306.2014.02.018
Citation: HUANG Zhi-li, HUANG Yan-jun, ZHANG Li-jun. Study on purification and characterization of superoxide dismutase in Trametes versicolor mycelium[J]. Science and Technology of Food Industry, 2014, (02): 120-123. DOI: 10.13386/j.issn1002-0306.2014.02.018

Study on purification and characterization of superoxide dismutase in Trametes versicolor mycelium

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  • Received Date: June 16, 2013
  • Superoxide dismutase ( SOD) was extracted from Trametes versicolor mycelium with membrane filtration, ammonium sulfate fractionation and SephadexG-75 gel column chromatography. The SOD was purified 12.6-fold with a specific activity of 4.61U/mg protein and a yield of 50.6%. The ultraviolet spectrum of purified SOD showed an absorbance peak at 257nm. The activity of enzyme was stable under 50℃ but decreased with higher temperature. Meanwhile, it was sensitive to chloroform-enthanol but inhibited obviously by H2 O2. These results indicated that the SOD from Trametes versicolor mycelium was Cu, Zn-SOD with a good thermostability.
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  • [1]
    Bannister J V, Bannister W H, Rotillio G.Aspects of the structure, function, and applications of superoxide dismutase[J].CRC Critical Review of Biochemistry, 1987, 22 (2) :111-180.
    [2]
    陈执中.超氧化物歧化酶 (SOD) 应用研究发展新趋势[C]//第六届全国SOD学术研讨会论文集[C].苏州:中国药学会生化与生物技术药物专业委员会, 2009:52-57.
    [3]
    崔凯, 王晓松, 马泽芳, 等.东北梅花鹿血液SOD纯化及部分性质的研究[J].中国农学通报, 2012, 28 (29) :34-38.
    [4]
    He Ning, Li Qingbiao, Sun Daohua, et al.Isolation, purification and characterization of superoxide dismutase form garlic[J].Biochemical Engineering Journal, 2008, 38 (1) :33-38.
    [5]
    Wang Shaoyun, Shao Biao, Liu Shutao, et al.Purification and characterization of Cu, Zn-superoxide dismutase from black soybean[J].Food Research International, 2012, 47 (2) :374-379.
    [6]
    Hong Y Li, Yu Zhao, Yang Cao, et al.Purification and characterization of a superoxide dismutase from Panax ginseng[J].Biomedical Chromatography, 2010, 24 (11) :1203-1207.
    [7]
    贺阳, 文连奎, 路鑫, 等.越橘叶超氧化物歧化酶的分离纯化与定性研究[J].食品科学, 2011, 32 (23) :206-209.
    [8]
    Jianguo Liu, Jingbing Wang, Mengmeng Yin, et al.Purification and characterization of superoxide dismutase from garlic[J].Food and Bioproducts Processing, 2011, 89:294-299.
    [9]
    张建新, 刘娜, 何桂梅, 等.大麦虫超氧化物歧化酶分离纯化及性质研究[J].食品科学, 2012, 33 (11) :215-218.
    [10]
    Brioukhanow A L, Nesatyy V J, Netrusov A I.Purification and characterization of Fe-containing superoxide dismutase from Methanobrevibacter arboriphilus Strain AZ[J].Biochemistry (Moscow) , 2006, 71 (4) :546-553.
    [11]
    程光宇, 王晓炜, 吴京燕, 等.16种食用菌超氧物歧化酶 (SOD) 活性、热稳定性及同工酶的研究[J].南京师大学报:自然科学版, 2006, 29 (4) :96-100.
    [12]
    黄志立, 黄彦君, 张丽君, 等.药用真菌菌丝体产SOD的研究初探[J].中国食用菌, 2006, 25 (3) :46-48.
    [13]
    戴玉成.中国东北地区木材腐朽菌的多样性[J].菌物学报, 2010 (29) :801-818.
    [14]
    祁永青, 刁治民, 刘涛.药用真菌云芝的研究概况[J].青海草业, 2008, 17 (3) :26-29.
    [15]
    苏瑛, 唐咏, 王成春.蛹虫草超氧化物歧化酶的分离纯化及性质研究[J].安徽农业科学, 2005, 33 (8) :1388-1389.
    [16]
    董爱文, 黄美娥, 唐克华, 等.两种野生乳菇中超氧化物岐化酶的研究[J].食品科学, 2006, 27 (4) :78-82.
    [17]
    周艳玲, 陈文颖, 赵雯, 等.北虫草超氧化物歧化酶的分离纯化及酶学特性[J].中国食用菌, 2010, 29 (4) :28-31.
    [18]
    黄志立, 黄彦君, 张丽君, 等.云芝菌丝体产SOD的深层发酵培养基的优化[J].中国食用菌, 2009, 28 (4) :41-43.
    [19]
    黄志立, 张丽君, 黄彦君, 等.云芝菌丝体产SOD的深层发酵培养条件的优化[J].食用菌, 2009 (4) :8-9.
    [20]
    Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing of the principle of dye binding[J].Analytical Biochemistry, 1976, 72:248-254.
    [21]
    Giannopolitis C N, Ries S K.Superoxide dismutaseⅡpurification and quantitative relationship with water-soluble protein in seedings[J].Plant Physiology, 1977, 59:315-318.
    [22]
    林庆斌, 廖升荣, 熊亚红, 等.超氧化物歧化酶 (SOD) 的研究和应用进展[J].化学世界, 2006 (6) :378-381.
    [23]
    Bowler C, Van C W, Van M M, et al.Superoxide dismutase in plants[J].Critical Reviews in Plant Science, 1994 (13) :199-218.

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