Study on the high cell density culture of Lactococcus lactis
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- Received Date:
November 19, 2013
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Abstract
In order to improve the cell density of Lactococcus lactis SYFS1.009, the fermentation culture medium and fed-batch culture conditions were studied. The optimum fermentation conditions by single-factor experiment and orthogonal experiment were determined:glucose 30g/L, yeast extract 40g/L, K2HPO440g/L, MgSO40.6g/L, MnSO4·H2O 0.1g/L, Tween 80 0.5mL/L. After cultivated in this medium for 14h at 30℃, DCW (dry cell weight) was 3.1g/L, which was about 3.4 times higher than in MRS (DCW 0.9g/L) . After 12h culture, the DCW was up to7.3g/L and OD600was 15.3.
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References
| [1] |
张广敏, 王炜, 包慧芳, 等.植物乳杆菌Lp2的高密度发酵[J].中国生物工程杂志, 2009, 29 (6) :68-73.
|
| [2] |
Osborne R J W.Production of frozen concentrated cheese starters by diffusion culture[J].Soc Diary Technol, 1977, 30:40-44.
|
| [3] |
Takahiro Suzuki.A dense cell culture system for microorganisms using a stirred ceramic membrane reactor incorporating asymmetric porous ceramic filters[J].Journal of fermentation and bioengineering, 1996, 82 (3) :264-271.
|
| [4] |
章新, 朱惠贤, 王林, 等.用快速分离柱高效液相色谱法生物发酵产物中的乳酸[J].中国酿造, 2005, 148 (7) :53-54.
|
| [5] |
张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社, 1994:168-169.
|
| [6] |
李志成, 张连斌, 段旭昌.嗜酸乳杆菌生长促进物质研究[J].中国食品学报, 2006, 6 (5) :40-44.
|
| [7] |
Luc De Vuyst, Erick J.Vandamme, Influence of the phosphorus and nitrogen source on nisin production in Lactococcus lactis subsp, lactis batch fermentations using a complex medium[J].Appl Microbiol Biotechnol, 1993, 40:17-22.
|
| [8] |
曹军卫, 马辉文.微生物工程[M].第二版.北京:科学出版社, 2007:80-88.
|
| [9] |
余龙江.发酵工程原理与技术应用[M].北京:化学工业出版社, 2006:9-11.
|
| [10] |
李寅, 高海军, 陈坚.高细胞密度发酵技术[M].北京:化学工业出版社, 2006:50-51.
|
| [11] |
郭淑文, 赵树欣, 崔景宜, 等.以乳清为原料采用混菌发酵技术促进Nisin生产的研究[J].中国酿造, 2010, 220 (7) :123-126.
|
-
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