The effect of PI3K/Akt pathway in the apoptosis of human hepatoma MMC-7721 cells induced by serum containing Iridoid glycosides from Boschniakia rossica

CUI Xiang-dan; Zheng Feng; ZHU Jie-bo; QUAN Ji-shu; YIN Xue-zhe

doi:10.13386/j.issn1002-0306.2018.01.014

Volume 39 Issue 1

Dec. 2017

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Science and Technology of Food Industry > 2018 > 39(1): 77-81. > DOI: 10.13386/j.issn1002-0306.2018.01.014

CUI Xiang-dan, Zheng Feng, ZHU Jie-bo, QUAN Ji-shu, YIN Xue-zhe. The effect of PI3K/Akt pathway in the apoptosis of human hepatoma MMC-7721 cells induced by serum containing Iridoid glycosides from Boschniakia rossica[J]. Science and Technology of Food Industry, 2018, 39(1): 77-81. DOI: 10.13386/j.issn1002-0306.2018.01.014

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The effect of PI3K/Akt pathway in the apoptosis of human hepatoma MMC-7721 cells induced by serum containing Iridoid glycosides from Boschniakia rossica

1. Affiliated Hospital of Yanbian University, Yanji 133000, China;
2. Yanbian University Medical College, Yanji 133000, China

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Received Date: June 15, 2017
Available Online: December 28, 2020

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Abstract

The aim of this study was to investigate the effect of Iridoid glycosides from Boschniakia rossica(IGBR)serum on the viability and apoptosis of human hepatoma SMMC-7721 cells, and to explore the possible mechanism. MTT method was used to detect the effect of IGBR containing serum on the viability of human hepatoma SMMC-7721 cells. Apoptosis was detected by flow cytometry, and Western blot was used to detect PI3K/Akt pathway related proteins. The results indicated that IGBR containing serum could reduce the viability of SMMC-7721 hepatoma cells and induce apoptosis. Fluorescence microscopy showed that apoptotic cells were enhanced by staining, and the fluorescence was bright, showing a uniform round and mass like structure. Western blot results compared with control group, 5-FU group and IGBR SMMC-7721 cells in the expression of Bax prtein increased, and p-Akt, reduced the Bcl-2 protein expression(p<0.05), no obvious difference between the two groups(p>0.05), while the total Akt did not change significantly. The IGBR containing serum can regulate Bax and Bcl-2 through PI3K/Akt signaling pathway and induce apoptosis of human hepatoma SMMC-7721 cells.
- Iridoid glycosides from Boschniakia rossica,
- apoptosis,
- PI3K/Akt

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Science and Technology of Food Industry

The effect of PI3K/Akt pathway in the apoptosis of human hepatoma MMC-7721 cells induced by serum containing Iridoid glycosides from Boschniakia rossica

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