Effect of Yam Gruel on Intestinal Permeability of Type 2 Diabetic Rats

HONG Xuepei; PANG Shuqin; GE Li; DAI Yanling; LIN Xinjun; LI Xia; WU Yilan; WANG Guanming; ZHANG Jiahui

doi:10.13386/j.issn1002-0306.2021060096

Volume 43 Issue 4

Feb. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(4): 379-386. > DOI: 10.13386/j.issn1002-0306.2021060096

HONG Xuepei, PANG Shuqin, GE Li, et al. Effect of Yam Gruel on Intestinal Permeability of Type 2 Diabetic Rats[J]. Science and Technology of Food Industry, 2022, 43(4): 379−386. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060096.

Citation:

PDF (2544 KB)

Effect of Yam Gruel on Intestinal Permeability of Type 2 Diabetic Rats

School of Nursing, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China

More Information

Received Date: June 09, 2021
Available Online: December 13, 2021

Graphical Abstract

Abstract

Abstract

Objective: To explore the effect of yam gruel on intestinal permeability in T2DM rats. Methods: 35 male Wistar rats were randomly divided into blank group (n=8) and model group (n=27). The rats in the blank group were given normal diet, and the model group were fed with high-fat diet and intraperitoneal injection of streptozotocin to prepare T2DM rat model. After modeling, the rats were randomly divided into model group, yam gruel group and metformin group. The blank group and model group were given 5 mL normal saline, the yam gruel group was given 5 mL yam gruel, and the metformin group was given 5 mL metformin. Fasting blood glucose (FBG) was measured every week. After the intervention, the histological morphology of colon was observed, and the protein expressions of TNF-α, IL-6, Occludin and ZO-1 in colon tissue, and the expression of Zonulin, LPS, TNF-α, IL-6 and fins in serum were detected. HOMA-IR was calculated. Results: In the model group, the intestinal villi were incomplete and sparsely spaced, accompanied by neutrophil exudation. The above situation in yam gruel group and metformin group was better than that in the model group. In the model group, the protein expression of TNF-α and IL-6 increased (P<0.05), the protein expression of Occludin and ZO-1 decreased (P<0.05), the expression of Zonulin, LPS, TNF-α, IL-6 and FINS increased (P<0.05), FBG and HOMA-IR increased (P<0.05). In the yam gruel group, the protein expression of TNF-α and IL-6 decreased (P<0.05), the protein expression of Occludin and ZO-1 increased (P<0.05), the expression of Zonulin, LPS, TNF-α, IL-6 and FINS decreased (P<0.05), FBG and HOMA-IR decreased (P<0.05). Conclusion: Yam gruel could reduce the intestinal permeability of T2DM rats, and then reduce the systemic inflammatory response, alleviate insulin resistance and improve blood glucose.
- yam gruel,
- type 2 diabetes,
- intestinal permea,
- inflammatory response,
- insulin resistance

FullText(HTML)

References (36)

References

[1]	中华医学会糖尿病学分会. 中国2型糖尿病防治指南(2020年版)[J]. 中华内分泌代谢杂志,2021,37(4):311−398. [Chinese Diabetes Society. Guideline for prevention and treatment of type 2 diabetes in China (2020 Edition)[J]. Chin J Endocrinol Metab,2021,37(4):311−398. doi: 10.3760/cma.j.cn311282-20210304-00142
[2]	万仁辉, 鲁瑾. 2型糖尿病大血管并发症的影响因素[J]. 第二军医大学学报,2020,41(1):75−80. [WAN Renhui, LU Jin. Influencing factors of macrovascular complications in type 2 diabetes mellitus[J]. Academic Journal of Second Military Medical University,2020,41(1):75−80.
[3]	WINER D A, LUCK H, TSAI S, et al. The intestinal immune system in obesity and insulin resistance[J]. Cell Metab,2016,23(3):413−426. doi: 10.1016/j.cmet.2016.01.003
[4]	THAISS C A, MAAYAN L, INNA G, et al. Hyperglycemia drives intestinal barrier dysfunction and risk for enteric infection[J]. Science, 2018, 359(Mar. 23 TN. 6382): 1376-1383.
[5]	韩煦, 姚政, 李俊燕, 等. 肠道免疫-慢性炎症与胰岛素抵抗及中医药治疗研究进展[J]. 中华中医药杂志,2019,34(6):2620−2623. [HAN Xu, YAO Zheng, LI Junyan, et al. Research progress on intestinal immunity-chronic inflammation, insulin resistance and traditional Chinese medicine treatment[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2019,34(6):2620−2623.
[6]	汪丽娟, 朱晓波. 肠道慢性炎症与2型糖尿病[J]. 生理科学进展,2018,49(6):443−447. [WANG Lijuan, ZHU Xiaobo. Chronic intestinal inflammation and type 2 diabetes mellitus[J]. Progress in Physiological Sciences,2018,49(6):443−447. doi: 10.3969/j.issn.0559-7765.2018.06.009
[7]	CHEN T, KIM CY, KAUR A, et al. Dietary fibre-based SCFA mixtures promote both protection and repair of intestinal epithelial barrier function in a Caco-2 cell model[J]. Food Funct,2017,8:1166−1173. doi: 10.1039/C6FO01532H
[8]	CHANG H K. Microbiota or short-chain fatty acids: which regulates diabetes?[J]. Cellular & Molecular Immunology, 2017, 15(2).
[9]	庞书勤, 辛惠明, 刘玲玉, 等. 薯蓣粥对2型糖尿病肠道内双歧杆菌及血糖影响研究[J]. 中国实用内科杂志,2017,37(3):247−250. [PANG Shuqin, XIN Huiming, LIU Lingyu, et al. Effect of yam gruel on bifidobacterium in the gut of patients with type 2 diabetes[J]. Chinese Journal of Practical Internal Medicine,2017,37(3):247−250.
[10]	洪雪珮, 庞书勤, 周建, 等. 薯蓣粥对T2DM大鼠肠道微生物、血糖、胰岛素的影响[J/OL]. 食品工业科技: 1−10 [2021-02-15]. https: //doi. org/10.13386/j. issn1002-0306.2020090192. HONG Xuepei, PANG Shuqin, ZHOU Jian, et al. Effects of Yam gruel on gut microflora, blood glucose and insulin in T2DM rats[J/OL]. Science and Technology of Food Industry: 1−10[2021-02-15]. https://doi.org/10.13386/j.issn1002-0306.2020090192.
[11]	徐叔云, 卞如镰, 陈修. 药理实验方法学[M]. 第4版, 北京: 人民卫生出版社, 2006: 1861. XU Shuyun, BIAN Rulian, CHEN Xiu. Methodology of pharmacological experiment[M]. 4th Edition, Beijing: People’s Health Publishing House, 2006: 1861.
[12]	林心君, 王麒又, 辛金钟, 等. 高成模率和高稳定性的糖尿病大鼠模型制备——高脂高糖膳食+STZ 体重联合体表面积法构建糖尿病大鼠模型[J]. 中国老年学杂志,2013,33(9):2051−2054. [LIN Xinjun, WANG Linyou, XIN Jinzhong, et al. The study of establishing a highly stable and successful diabetic rat model[J]. Chinese Journal of Gerontology,2013,33(9):2051−2054. doi: 10.3969/j.issn.1005-9202.2013.09.031
[13]	蒋宛瑾, 谢聪, 喻嵘, 等. 白虎加人参汤对转基因2型糖尿病MKR小鼠肠道TLR4/NF-κB信号通路及肠道屏障功能的影响[J]. 中草药,2020,51(11):3005−3012. [JIANG Wanjin, XIE Cong, YU Rong, et al. Effect of Baihu and Renshen decoction on intestinal TLR4/NF-κB signaling pathway and intestinal barrier in type 2 diabetes MKR mice[J]. Chinese Traditional and Herbal Drugs,2020,51(11):3005−3012.
[14]	于爽. NLRP3炎症小体参与食管鳞癌增殖、侵袭和迁移并介导荷瘤小鼠顺铂抵抗[D]. 郑州: 郑州大学, 2020. YU Shuang. NLRP3 inflammasome participates in the proliferation, invasion and migration of esophageal squamous cell carcinoma and mediates cisplatin resistance in tumor-bearing mice[D]. Zhengzhou: Zhengzhou University, 2020.
[15]	许女, 郭宏萍, 杨光, 等. 山西老陈醋源植物乳杆菌173对Ⅱ型糖尿病大鼠的降血糖机制[J]. 中国食品学报,2020,20(12):131−140. [XU Nv, GUO Hongping, YANG Guang, et al. Hypoglycemic mechanism of Lactobacillus plantarum 173 from Shanxi, Chen Cuyuan, in type 2 diabetic rats[J]. Journal of Chinese Institute of Food Science and Technology,2020,20(12):131−140.
[16]	王语聪, 谢智鑫, 张学艳, 等. 阿拉伯半乳聚糖对2型糖尿病大鼠肠道碱性磷酸酶、细菌内毒素和肠道中细胞因子的影响[J/OL]. 食品工业科技: 1−13 [2021-02-19]. https: //doi. org/10.13386/j. issn1002-0306.2020090167. WANG Yucong, XIE Zhixin, ZHANG Xueyan, et al. Effect of arabinogalactan on intestinal alkaline phosphatase, bacterial endotoxin and serum cytokines in type 2 diabetic rats[J/OL]. Science and Technology of Food Industry: 1−13[2021-02-19].https://doi.org/10.13386/j.issn1002-0306.2020090167.
[17]	ROHR M W , NARASIMHULU C A , RUDESKI-ROHR T A , et al. Negative effects of a high-fat diet on intestinal permeability: A review[J]. Advances in Nutrition, 2019, 11(1).
[18]	WANG R X, LEE J S, CAMPBELL E L, et al. Microbiota-derived butyrate dynamically regulates intestinal homeostasis through regulation of actin-associated protein synaptopodin[J]. Proceedings of the National Academy of Sciences,2020,117(21):201917597.
[19]	刘颖, 常志尚, 徐宏伟, 等. 糖尿病大鼠肠黏膜屏障损伤检测方法的评价及蜂胶降糖作用的探讨[J]. 中国比较医学杂志,2019,29(3):30−36. [LIU Ying, CHANG Zhishang, XU Hongwei, et al. Evaluation of methods for detecting intestinal mucosal barrier damage and exploration of the hypoglycemic effect of propolis in diabetic rats[J]. Chin J Comp Med,2019,29(3):30−36. doi: 10.3969/j.issn.1671-7856.2019.03.005
[20]	LI Pan, XIAO Nan, ZENG Liping, et al. Structural characteristics of a mannoglucan isolated from Chinese yam and its treatment effects against gut microbiota dysbiosis and DSS-induced colitis in mice[J]. Carbohydrate Polymers,2020:250.
[21]	HISAKO KAYAMA, RYU OKUMURA, KIYOSHI TAKEDA. Interaction between the microbiota, epithelia, and immune cells in the intestine[J]. Annual Review of Immunology,2020:38.
[22]	TONG L C, WANG Y, WANG Z B, et al. Propionate ameliorates dextran sodium sulfate-induced colitis by improving intestinal barrier function and reducing inflammation and oxidative stress[J]. Front Pharmacol,2016,7:253.
[23]	袁本银, 马坤岭. Zonulin在糖脂代谢紊乱中作用的研究进展[J]. 东南大学学报(医学版),2019,38(3):532−535. [YUAN Benyin, MA Kunling. Research progress of zonulin in the disorder of glucose and lipid metabolism[J]. Journal of Southeast University(Medical Science Edition),2019,38(3):532−535.
[24]	JIANG Wanjin, XIE Cong, YU Rong, et al. Effect of Baihu and Renshen decoction on intestinal TLR4/NF-κB signaling pathway and intestinal barrier in type 2 diabetes MKR mice[J]. Chinese Traditional and Herbal Drugs,2020,51(11):3005−3012.
[25]	朱佳杰, 刘珊, 郭宇, 等. 姜黄素对肠道屏障的作用及机制[J]. 中国中西医结合消化杂志,2021,29(2):147−150. [ZHU Jiajie, LIU Shan, GUO Yu, et al. Effect and mechanisms of curcumin on intestinal barrier function[J]. Chinese Journal of Integrated Traditional and Western Medicine on Digestion,2021,29(2):147−150. doi: 10.3969/j.issn.1671-038X.2021.02.14
[26]	LI B, XU P, WU S, et al. Diosgenin attenuates lipopolysaccharide-induced parkinson’s disease by inhibiting the TLR/NF-κB pathway[J]. Journal of Alzheimers Disease Jad,2018,64(3):1−13.
[27]	ZAHRA KIASALARI, TAYEBEH RAHMANI, NARGES MAHMOUDI, et al. Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation[J]. Biomedicine & Pharmacotherapy,2017,86:654−661.
[28]	KIM Y A, KEOGH J B, CLIFTON P M. Probiotics, prebiotics, synbiotics and insulin sensitivity[J]. Nutrition Research Reviews,2017:1−17.
[29]	KOH A, DE VADDER F, KOVATCHEVA-DATCHARY P, et al. From dietary fiber to host physiology: Short-chain fatty acids as key bacterial metabolites[J]. Cell,2016,165(6):1332−1345. doi: 10.1016/j.cell.2016.05.041
[30]	FUJISAKA S, USUI I, NAWAZ A, et al. Bofutsushosan improves gut barrier function with a bloom of Akkermansia muciniphila and improves glucose metabolism in mice with diet-induced obesity[J]. Scientific Reports,2020,10(1):1−13. doi: 10.1038/s41598-019-56847-4
[31]	张小雨, 高源, 齐云. 代谢性炎症在代谢综合征中的作用[J]. 生命科学,2021,33(1):15−25. [ZHANG XiaoYu, GAO Yuan, QI Yun. The role of metaflammation in metabolic syndrome[J]. Chinese Bulletin of Life Sciences,2021,33(1):15−25.
[32]	魏伊秋, 李满, 余佳. 慢性炎症与胰岛素抵抗机制关系的研究进展[J]. 临床与病理杂志,2019,39(3):640−645. [WEI Yiqiu, LI Man, YU Jia. Research progress in the relationship between chronic inflammation and insulin resistance[J]. Journal of Clinical and Pathological Research,2019,39(3):640−645. doi: 10.3978/j.issn.2095-6959.2019.03.030
[33]	SHAUN SABICO, AYAH AL-MASHHARAWI, NASSER M. AL-DAGHRI, et al. Effects of a 6-month multi-strain probiotics supplementation in endotoxemic, inflammatory and cardiometabolic status of T2DM patients: A randomized, double-blind, placebo-controlled trial[J]. Clinical Nutrition,2019,38(4):1561−1569. doi: 10.1016/j.clnu.2018.08.009
[34]	植飞, 邢琪昌, 汪莹, 等. 佛手山药多糖对2型糖尿病大鼠糖脂代谢及氧化应激的影响[J]. 食品科学,2017,38(5):262−266. [ZHI Fei, XING Qi-chang, WANG Ying, et al. Effect of Dioscorea opposita Thunb. polysaccharide on glycolipid metabolism and oxidative stress in type 2 diabetic rats[J]. Food Science,2017,38(5):262−266. doi: 10.7506/spkx1002-6630-201705043
[35]	CAI Shengyu, CHEN Jiaxu, LI Yousheng. Dioscin protects against diabetic nephropathy by inhibiting renal inflammation through TLR4/NF-κB pathway in mice[J]. Immunobiology,2020,225(3):151941. doi: 10.1016/j.imbio.2020.151941
[36]	曹泽标, 刘超男, 王保华, 等. 基于“火热论”探讨“胰岛素抵抗-胰岛β细胞缺陷-高血糖-2型糖尿病”网[J]. 中华中医药杂志,2019,34(8):3668−3671. [CAO Zebiao, LIU Chaonan, WANG Baohua, et al. Discussion on network of ‘insulin resistance-pancreatic beta cell defect-hyperglycemiaT2DM’ based on ‘fire-heat theory’[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2019,34(8):3668−3671.

Supplements (0)

Get Citation

PDF

XML

Article Metrics

Article views (182) PDF downloads (10)

Science and Technology of Food Industry

Effect of Yam Gruel on Intestinal Permeability of Type 2 Diabetic Rats

Abstract

References

Catalog

Article Metrics

Export File

Citation

Format

Content