REN Haojie, DENG Kangyan, LU Yongcun, et al. Effects of Modified Whey Protein on Quality and Cholate Binding Ability of Set Yoghurt[J]. Science and Technology of Food Industry, 2023, 44(2): 123−133. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040130.
Citation: REN Haojie, DENG Kangyan, LU Yongcun, et al. Effects of Modified Whey Protein on Quality and Cholate Binding Ability of Set Yoghurt[J]. Science and Technology of Food Industry, 2023, 44(2): 123−133. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040130.

Effects of Modified Whey Protein on Quality and Cholate Binding Ability of Set Yoghurt

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  • Received Date: April 12, 2022
  • Available Online: November 16, 2022
  • In this study, whey protein (WPI-E+MA) modified with enzyme combined malic acid was added to set yoghurt in order to improve the texture and bile salt-binding ability. The effects of WPI-E+MA on quality, gel structure and cholate binding ability of set yoghurt were evaluated. The results showed that WPI-E+MA could improve the water holding capacity, apparent viscosity, shear stress and viscoelasticity of set yoghurt. And the optimal value was obtained at the addition amount of 8%. Besides, the gel mesh crosslinked structure of yogurt became more denser and had higher degree of polymerization with the addition of WPI-E+MA. The binding rate of sodium deoxycholate (SDC), sodium cholate (SC) and sodium taurocholate (STC) also reached the maximum value at 8%. WPI-E+MA could effectively improve the quality of set yoghurt and enhance the cholate binding ability.
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  • [1]
    姜池. 益生菌对高胆固醇血症的缓解作用及机制研究[D]. 无锡: 江南大学, 2021

    JIANG C. Study on preparation and property of biological active peptides derived from whey protein with capacity of decreasing the level of cholesterol in serum[D]. Wuxi: Jiangnan University, 2021.
    [2]
    刘培玲, 张晴晴, 高增丽, 等. 乳清蛋白改性研究进展[J]. 食品科学,2021,42(33):333−348. [LIU P L, ZHANG Q Q, GAO Z L, et al. Advances in modification of whey proteins[J]. Food Science,2021,42(33):333−348.
    [3]
    NAGAOKA S, KANAMARU Y, KAZUYA Y. Effects of whey protein and casein on the plasma and liver lipids in rats[J]. Agricultural & Biological Chemistry,1991,55:813−818.
    [4]
    NAGAOKA S, FUTAMURA Y, MIWA K, et al. Identification of novel hypocholesterolemic peptides derived from bovine milk beta-lactoglobulin[J]. Biochemical and Biophysical Research Communications,2001,281(1):11−17. doi: 10.1006/bbrc.2001.4298
    [5]
    高学飞. 乳清蛋白源降胆固醇活性肽的制备及其生物学功能的研究[D]. 合肥: 安徽农业大学, 2006.

    GAO X F. Alleviating effects of probiotics on hypercholesterolemia and potential mechanisms[D]. Hefei: Anhui Agricultural University, 2006.
    [6]
    孙丹丹. 乳清蛋白降胆固醇肽的制备及活性研究[D]. 哈尔滨: 东北农业大学, 2017.

    SUN D D. Study on the preparation and activity of hypocholesterolemic peptides from whey protein[D]. Harbin: Northeast Agricultural University, 2017.
    [7]
    REMEUF F, MOHAMMED S, SODINI I, et al. Preliminary observations on the effects of milk fortification and heating on microstructure and physical properties of stirred yogurt[J]. International Dairy Journal,2003,13(9):773−782. doi: 10.1016/S0958-6946(03)00092-X
    [8]
    GYAWALI R, IBRAHIM S A. Effects of hydrocolloids and processing conditions on acid whey production with reference to Greek yogurt[J]. Trends in Food Science & Technology,2016:61−76.
    [9]
    CHANDAN R C, O'RELL K R. Manufacturing yogurt and fermented milks[M]. Blackwell, 2007.
    [10]
    曾羲. 大豆分离蛋白酶解液对酸乳发酵及产胞外多糖的影响研究[D]. 广州: 华南理工大学, 2015.

    ZENG X. Fermentation process of soy isolated protein hydrolysates and EPS produce on yoghurt[D]. Guangzhou: South China University of Technology, 2015.
    [11]
    李晶晶. 荞麦酶解液植物乳杆菌发酵乳生产工艺及抗氧化活性分析[D]. 上海: 上海师范大学, 2015.

    LI J J. Study on production technology and antioxidant activity of Lactobacillus plantarum fermented milk from buckwheat enzymatic hydrolysate[J]. Shanghai: Shanghai Normal University, 2015.
    [12]
    张国柱, 闫碧轩, 贾亚婷, 等. 乳清分离蛋白酶解物对酸奶品质与抗氧化能力的影响[J]. 食品工业,2019,40(3):35−38. [ZHANG G Z, YAN B X, JIA Y T, et al. Effect of whey protein hydrolysate on the quality and antioxidant ability of yogurt[J]. The Food Industry,2019,40(3):35−38.
    [13]
    MEI L, FU Q, GUO T, et al. Structural changes and cholesterol-lowering in denatured whey protein isolate: Malic acid combined enzymolysis[J]. Food Hydrocolloids, 2022: 107502.
    [14]
    霍辰辰, 胡志和, 鲁丁强, 等. 添加谷氨酰胺转氨酶对凝固型酸奶品质的影响[J]. 食品工业科技,2022,43(11):83−95. [HUO C C, HU Z H, LU D Q, et al. Effect of adding glutamine transaminase on the quality of set-style yogurt[J]. Science and Technology of Food Industry,2022,43(11):83−95. doi: 10.13386/j.issn1002-0306.2021090097
    [15]
    李荣华. 乳蛋白对凝固型酸奶流变学特性及微观结构的影响[D]. 哈尔滨: 东北农业大学, 2007.

    LI R H. Effect of milk protein on rheological prorerties and microstructure of ret yoguhrt[D]. Harbin: Northeast Agricultural University, 2007.
    [16]
    BEVERIDGE T, TOMA S J, NAKAI S. Determination of SH- and SS-Groups in some food proteins using Ellman's Reagent[J]. Journal of Food Science,1974,39(1):49−51. doi: 10.1111/j.1365-2621.1974.tb00984.x
    [17]
    SHEN X, FANG T, GAO F, et al. Effects of ultrasound treatment on physicochemical and emulsifying properties of whey proteins pre- and post-thermal aggregation[J]. Food Hydrocolloids,2016,63:668−676.
    [18]
    LIU G, LI J, SHI K, et al. Composition, secondary structure, and self-assembly of oat protein isolate[J]. Journal of Agricultural & Food Chemistry,2009,57(11):4552−4558.
    [19]
    HU H, LI-CHAN E C Y, WAN L, et al. The effect of high intensity ultrasonic pre-treatment on the properties of soybean protein isolate gel induced by calcium sulfate[J]. Food Hydrocolloids,2013,32(2):303−311. doi: 10.1016/j.foodhyd.2013.01.016
    [20]
    HEMAR Y, LIU L H, MEUNIER N, et al. The effect of high hydrostatic pressure on the flow behaviour of skim milk-elatin mixtures[J]. Innovative Food Science & Emerging Technologies,2010,11(3):432−440.
    [21]
    YOSHIE-STARK Y, WAESCHE A. In vitro binding of bile acids by lupin protein isolates and their hydrolysates[J]. Food Chemistry,2004,88(2):179−184. doi: 10.1016/j.foodchem.2004.01.033
    [22]
    李靖. 灵芝白灵菇酸奶的研制及发酵液组分对酸奶品质的影响[D]. 泰安: 山东农业大学, 2012.

    LI J. Development of the Ganoderma and Pleurotus nebrodensis yoghurt and the effect of fermented broth composition on yoghurt quality[D]. Taian: Shandong Agricultural University, 2012.
    [23]
    高奇. 凝胶型酸乳的制备及其质构流变特性研究[D]. 无锡: 江南大学, 2006.

    GAO Q. Study on preparation of gel yoghurt and its TPA and rheological characteristic[D]. Wuxi: Jiangnan University, 2006.
    [24]
    YADAV J S S, YAN S, PILLI S, et al. Cheese whey: A potential resource to transform into bioprotein, functional/nutritional proteins and bioactive peptides[J]. Biotechnology Advances,2015,33(6):756−774. doi: 10.1016/j.biotechadv.2015.07.002
    [25]
    蔡自建, 刘鲁蜀, 陈炼红. 强化乳清浓缩蛋白对酸奶品质影响的研究[J]. 西南民族大学学报(自然科学版),2012,38(2):242−246. [CAI Z J, LIU L S, CHEN L H. Quality of yogurt supplemented with whey protein concentrate[J]. Journal of Southwest Minzu University (Natural Science Edition),2012,38(2):242−246.
    [26]
    GUZMÁN-GONZÁLEZ MARTA, MORAIS F, AMIGO L. Influence of skimmed milk concentrate replacement by dry dairy products in a low-fat set-type yoghurt model system. Use of caseinates, co-precipitate and blended dairy powders[J]. Journal of the Science of Food & Agriculture,2000,80(4):433−438.
    [27]
    SODINI I, MATTAS J, TONG P S. Influence of pH and heat treatment of whey on the functional properties of whey protein concentrates in yoghurt[J]. International Dairy Journal,2006,16(12):1464−1469. doi: 10.1016/j.idairyj.2005.03.014
    [28]
    王月秀. 添加乳清浓缩蛋白(WPC)对发酵乳饮料稳定性影响的研究[D]. 杭州: 浙江工商大学, 2013.

    WANG Y X. Studies on the stability of fermented milk beverage by the addition of whey protein concentrate[D]. Hangzhou: Zhejiang Gongshang University, 2013.
    [29]
    孙颖. 酪蛋白与乳清蛋白比例对乳蛋白结构的影响[D]. 哈尔滨: 东北农业大学, 2009.

    SUN Y. Structure characteristics of milk proteins complexes with altered casein to protein ratios[D]. Harbin: Northeast Agricultural University, 2009.
    [30]
    赵谋明, 源博恩, 罗东辉, 等. 酸性条件下大豆分离蛋白的亚基解离[J]. 华南理工大学学报(自然科学版),2011,39(9):22−27. [ZHAO M M, YUAN B E, LUO D H, et al. Subunit dissociation of soybean protein isolates in acid conditions[J]. Journal of South China University of Technology (Natural Science Edition),2011,39(9):22−27.
    [31]
    EVANGELHO J A, VANIER N L, PINTO V Z, et al. Black bean (Phaseolus vulgaris L.) protein hydrolysates: Physicochemical and functional properties[J]. Food Chemistry,2017,214:460−467. doi: 10.1016/j.foodchem.2016.07.046
    [32]
    WANG J, SHIMADA M, KATO Y, et al. Cholesterol-lowering effect of rice bran protein containing bile acid-binding proteins[J]. Bioscience, Biotechnology, and Biochemistry,2015,79(3):456−461. doi: 10.1080/09168451.2014.978260
    [33]
    DAMIN M R, ALCANTARA M R, NUNES A P, et al. Effects of milk supplementation with skim milk powder, whey protein concentrate and sodium caseinate on acidification kinetics, rheological properties and structure of nonfat stirred yogurt[J]. LWT-Food Science and Technology,2009,42(10):1744−1750. doi: 10.1016/j.lwt.2009.03.019
    [34]
    MATUMOTO-PINTRO P T, RABIEY L, ROBITAILLE G, et al. Use of modified whey protein in yoghurt formulations[J]. International Dairy Journal,2011,21(1):21−26. doi: 10.1016/j.idairyj.2010.07.003
    [35]
    LEE W J, LUCEY J A. Rheological properties, whey separation, and microstructure in set-style yoghurt: Effects of heating temperature and incubation temperature[J]. Journal of Texture Studies,2004,34(5−6):515−536.
    [36]
    AKALIN A S, UNAL G, DINKCI N, et al. Microstructural, textural, and sensory characteristics of probiotic yogurts fortified with sodium calcium caseinate or whey protein concentrate[J]. Journal of Dairy Science,2012,95(7):3617−3628. doi: 10.3168/jds.2011-5297
    [37]
    PUVANENTHIRAN A, WILLIAMS R P W, AUGUSTIN M A. Structure and visco-elastic properties of set yoghurt with altered casein to whey protein ratios[J]. International Dairy Journal,2002,12(4):383−391. doi: 10.1016/S0958-6946(02)00033-X
    [38]
    BEAULIEU M, POULIOT Y, POULIOT M. Thermal aggregation of whey proteins in model solutions as affected by casein/whey protein ratios[J]. Journal of Food Science,2010,64(5):776−780.
    [39]
    TORRES I C, RUBIO J M A, IPSEN R. Using fractal image analysis to characterize microstructure of low-fat stirred yoghurt manufactured with microparticulated whey protein[J]. Journal of Food Engineering,2012,109(4):721−729. doi: 10.1016/j.jfoodeng.2011.11.016
    [40]
    TOBIN J T, FITZSIMONS S M, KELLY A L, et al. Microparticulation of mixtures of whey protein and inulin[J]. International Journal of Dairy Technology,2010,63(1):32−40. doi: 10.1111/j.1471-0307.2009.00550.x
    [41]
    SANCHEZ C, POULIOT M, GAUTHIER S F, et al. Thermal aggregation of whey protein isolate containing microparticulated or hydrolyzed whey proteins[J]. Journal of Agricultural & Food Chemistry,1997,45(7):2384−2392.
    [42]
    MCCLEMENTS D J, KEOGH M K, et al. Physical properties of cold-setting gels formed from heat-denatured whey protein isolate[J]. Journal of the Science of Food & Agriculture,1995,69(1):7−14.
    [43]
    魏月媛, 李理. 火麻仁油凝固型酸奶的品质研究[J]. 现代食品科技,2015(6):198−204. [WEI Y Y, LI L. Preparation and evaluation of solidifying yoghurt supplemented with hemp seed oil[J]. Modern Food Science and Technology,2015(6):198−204.
    [44]
    GUINEE T P, MULLINS C G, REVILLE W J, et al. Physical properties of stirred-curd unsweetened yoghurts stabilised with different dairy ingredients[J]. Milchwissenschaft-milk Science International,1995,50(4):196−200.
    [45]
    KÜCÜKCETIN A. Effect of heat treatment of skim milk and final fermentation pH on graininess and roughness of stirred yogurt[J]. International Journal of Dairy Technology,2008,61(4):385−390. doi: 10.1111/j.1471-0307.2008.00420.x
    [46]
    LUCEY J A, MUNRO P A, SINGH H. Effects of heat treatment and whey protein addition on the rheological properties and structure of acid skim milk gels[J]. International Dairy Journal,1999,9(3−6):275−279. doi: 10.1016/S0958-6946(99)00074-6
    [47]
    WALSTRA P. Relation between structure and texture of cultured milk products[J]. Journal of the Association of Physicians of India, 1998, 18(1): 9−15.
    [48]
    CHO Y H, LUCEY J A, SINGH H. Rheological properties of acid milk gels as affected by the nature of the fat globule surface material and heat treatment of milk[J]. International Dairy Journal,1999,9(8):537−545. doi: 10.1016/S0958-6946(99)00123-5
    [49]
    彭新颜, 许晶, 杨阳, 等. 乳清多肽对猪肉糜氧化和凝胶特性的影响[J]. 食品科学,2016,37(21):31−37. [PENG X Y, XU J, YANG Y, et al. Effect of whey protein peptides on lipid oxidation and gel properties of pork patties[J]. Food Science,2016,37(21):31−37. doi: 10.7506/spkx1002-6630-201621006
    [50]
    PETRACCI MASSIMILIANO, BIANCHI MAURIZIO, MUDALAL SAMER, et al. Functional ingredients for poultry meat products[J]. Trends in Food science & Technology,2013,33(1):27−39.
    [51]
    KINSELLA J E, WHITEHEAD D M. Proteins in whey: Chemical, physical, and functional properties[J]. Advances in Food and Nutrition Research,1989,33:343−438.
    [52]
    余娱乐. pH调节法制备兔肉分离蛋白及其性质研究[D]. 重庆: 西南大学, 2018.

    YU Y L. Study on preparation and properties of protein isolates from rabbit by pH-shifting[D]. Chongqing: Southwest University, 2018.
    [53]
    王丹, 刘景圣, 刘回民, 等. 乳酸菌中胆盐水解酶的研究进展[J]. 农产品加工(学刊),2011,4:33−37. [WANG D, LIU J S, LIU H M, et al. Research progress of the bile salt hydrolyses enzymes in lactic acid bacteria[J]. The Processing of Agricultural Products,2011,4:33−37.

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