Simultaneous Determination of Nitrite and Ascorbic Acid in Fruits and Vegetables by Differential Pulse Voltammetry

CHEN Linlin; SONG Jiaqi; WANG Ling; YANG Xiyao; ZHENG Fengming; ZHANG jiaxin; ZHANG Na

doi:10.13386/j.issn1002-0306.2022040260

Volume 44 Issue 5

Feb. 2023

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Science and Technology of Food Industry > 2023 > 44(5): 267-276. > DOI: 10.13386/j.issn1002-0306.2022040260

CHEN Linlin, SONG Jiaqi, WANG Ling, et al. Simultaneous Determination of Nitrite and Ascorbic Acid in Fruits and Vegetables by Differential Pulse Voltammetry[J]. Science and Technology of Food Industry, 2023, 44(5): 267−276. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040260.

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Simultaneous Determination of Nitrite and Ascorbic Acid in Fruits and Vegetables by Differential Pulse Voltammetry

College of Food Engineering, Harbin University of Commerce, Harbin 150028, China

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Received Date: April 23, 2022
Available Online: January 02, 2023

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Abstract

Hydroquinone (HQ) was used to catalyze Methanobactin (Mb) to reduce chlorauric acid to prepare Gold Nanoparticles (AuNPs). Mb@AuNPs was modified onto the surface of a bare gold electrode via electrodeposition to prepare the Mb@AuNPs/Au electrode. Subsequently, differential pulse voltammetry was conducted for the simultaneous determination of nitrite and ascorbic acid concentrations as well as condition optimization. For the electrode assembly, electrodeposition was performed with a 0.20 mol/L and pH6.5 PBS at a 100 mV/s scan rate for 40 cycles. In the linear range of 2~5600 μmol/L and 1~6000 μmol/L, the oxidation peak current of both nitrite and ascorbic acid detected by Mb@AuNPs/Au showed a good linear relationship with the concentration (R²>0.9928), and the limit of detection (LOD) was 0.31 and 0.57 μmol/L, respectively. The recovery rates of standard addition for the actual samples were 92.59%~109.26%, 90.01%~103.51%. The method has good reproducibility and stability and can be used for simultaneous determination of nitrite and ascorbic acid in fruits and vegetables.
- nitrite,
- ascorbic acid,
- differential pulse voltammetry,
- modified electrode

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Science and Technology of Food Industry

Simultaneous Determination of Nitrite and Ascorbic Acid in Fruits and Vegetables by Differential Pulse Voltammetry

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