Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP

LU Yajun; LIU Ying; WANG Yi; HUANG Wen

doi:10.13386/j.issn1002-0306.2022070173

Volume 44 Issue 12

Jun. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(12): 397-404. > DOI: 10.13386/j.issn1002-0306.2022070173

LU Yajun, LIU Ying, WANG Yi, et al. Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP[J]. Science and Technology of Food Industry, 2023, 44(12): 397−404. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070173.

Citation:

PDF (1780 KB)

Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP

LU Yajun^1,,
LIU Ying^{1, 2},
WANG Yi¹,
HUANG Wen^{1, 2, ,}

1.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2.
Key Laboratory of Fruit and Vegetable Processing and Quality Control in Hubei Province, Wuhan 430070, China

More Information

Received Date: July 18, 2022
Available Online: April 18, 2023

Graphical Abstract

Abstract

Abstract

Objective: The protective effect of polyphenol of lotus seed epicarp on HepG2 oxidative stress damage induced by tert-butylhydro-peroxide (T-BHP) was investigated. Method: The concentration of T-BHP with a survival rate close to 50% was selected as the concentration for establishing the oxidative damage model. To evaluate the anti-oxidative stress activity levels of polyphenol of lotus seed epicarp at different concentrations (2.5, 5, and 7.5 μg/mL), cell viability, reactive oxygen species (ROS), malondialdehyde (MDA), lactic dehydrogenase (LDH), glutathione (GSH) and antioxidant enzyme-related gene expression were used as evaluation indicators, and tea polyphenol was used as the positive control. Result: When 120 μmol/L concentration of T-BHP was established for 4 h, the cell survival rate reached 49.18%±7.55%, which met the requirements of model construction. Therefore, 120 μmol/L was selected as the modeling concentration of the oxidative damage model for subsequent experiments. The polyphenol from lotus seed epicarp significantly inhibited the decrease of cell survival rate induced by T-BHP (P<0.01). At 7.5 μg/mL, the ROS level decreased by 45.99%, the MDA decreased by 58.77%, the LDH decreased by 71.61%, and the GSH increased by 206.60% (P<0.05). The expression of antioxidant enzyme-related genes including CuZnSOD, GCLC, GPx, and GSH also gradually returned to the normal level, all in a dose-dependent manner. Conclusion: The polyphenol of lotus seed epicarp protected against the oxidative damage induced by T-BHP in HepG2 cells. The mechanism might be related to the increase of intracellular antioxidant enzyme activity and the clearance of excessive ROS.
- polyphenol of lotus seed epicarp,
- HepG2,
- anti-oxidative stress,
- reactive oxygen species,
- tert-butylhydro-peroxide

FullText(HTML)

References (35)

References

[1]	MOLDOGAZIEVA N T, MOKHOSOEV I M, FELDMAN N B, et al. ROS and RNS signalling: Adaptive redox switches through oxidative/nitrosative protein modifications[J]. Free Radical Research,2018,52(5):507−543. doi: 10.1080/10715762.2018.1457217
[2]	PISOSCHI A M, POP A. The role of antioxidants in the chemistry of oxidative stress: A review[J]. European Journal of Medicinal Chemistry,2015,97:55−74. doi: 10.1016/j.ejmech.2015.04.040
[3]	AITKENR J, KOPPERS A J. Apoptosis and DNA damage in human spermatozoa[J]. Asian Journal of Andrology,2011,13(1):36−42. doi: 10.1038/aja.2010.68
[4]	ODATSUT, KUROSHIMA S, SHINOHARA A, et al. Lactoferrin with Zn-ion protects and recovers fibroblast from H₂O₂-induced oxidative damage[J]. International Journal of Biological Macromolecules,2021,190:368−374. doi: 10.1016/j.ijbiomac.2021.08.214
[5]	李霞, 马宁, 田晶, 等. 氧化应激与组织损伤的研究进展[J]. 吉林医药学院学报,2020,41(4):292−294. [LI X, MA N, TIAN J, et al. Progress on oxidative stress and tissue damage[J]. Journal of Jilin Medical University,2020,41(4):292−294. doi: 10.13845/j.cnki.issn1673-2995.2020.04.022 LI X, MA N, TIAN J, et al. Progress on oxidative stress and tissue damage[J]. Journal of Jilin Medical University, 2020, 41(4): 292-294. doi: 10.13845/j.cnki.issn1673-2995.2020.04.022
[6]	SHARMA A,SHAHZAD B,REHMAN A,et al. Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress[J]. Molecules,2019,24(13):2452−2474. doi: 10.3390/molecules24132452
[7]	YAHFOUF N, ALSADI N, JAMBI M, et al. The immunomodulatory and anti-inflammatory role of polyphenols[J]. Nutrients,2018,10(11):1618−1641. doi: 10.3390/nu10111618
[8]	MARı´A G A, SONIA D P T, CELESTINO S B, et al. Evaluation of the antioxidant properties of fruits[J]. Food Chemistry,2004,84(1):13−18. doi: 10.1016/S0308-8146(03)00160-2
[9]	CHEN G L, FAN M X, WU J L, et al. Antioxidant and anti-inflammatory properties of flavonoids from lotus plumule[J]. Food Chemistry,2019,277:706−712. doi: 10.1016/j.foodchem.2018.11.040
[10]	廖霞, 郑少杰, 卢可可, 等. 植物多酚通过Nrf2/ARE信号通路抗氧化作用研究进展[J]. 食品科学,2016,37(7):227−232. [LIAO X, ZHENG S J, LU K K, et al. Plant polyphenols exert antioxidant activity of by Nrf2/ARE signaling pathway: A review[J]. Food Science,2016,37(7):227−232. doi: 10.7506/spkx1002-6630-201607041 LIAO X, ZHENG S J, LU K K, et al. Plant polyphenols exert antioxidant activity of by Nrf2/ARE signaling pathway: A review[J]. Food Science, 2016, 37(7): 227-232. doi: 10.7506/spkx1002-6630-201607041
[11]	蒋蕾. 莲子皮渣中酚类物质及其抗氧化活性研究[D]. 杭州: 浙江大学, 2019. JIANG L. Research on phenolic compounds and the antioxidant activity of lotus seed epicarp residue[D]. Hangzhou: Zhejiang University, 2019.
[12]	MA Z L, HUANG Y, HUANG W, et al. Separation, identification, and antioxidant activity of polyphenols from lotus seed epicarp[J]. Molecules,2019,24(21):4007−4017. doi: 10.3390/molecules24214007
[13]	YAN Z, LUO X P, CONG J L, et al. Subcritical water extraction, identification and antiproliferation ability on HepG2 of polyphenols from lotus seed epicarp[J]. Industrial Crops and Products,2019,129:472−479. doi: 10.1016/j.indcrop.2018.12.031
[14]	马双双. 莲子壳多酚的提取、分离纯化、结构鉴定及抗氧化活性研究[D]. 武汉: 华中农业大学, 2013. MA S S. Study on extraction, purification, identification and antioxidant activity of polyphenols from lotus seed epicarp[D]. Wuhan: Huazhong Agricultural University, 2013.
[15]	崔阳, 李天竹, 刘子琦, 等. 荞麦壳抗氧化活性组分对HepG2细胞氧化损伤的改善作用[J]. 吉林农业大学学报: 1−11 [2023-04-06]. DOI: 10.13327/j. jjlau. 2020.5510 CUI Y, LI T Z, LIU Z Q, et al. Amelioration of antioxidative fraction from buckwheat hulls against oxidative stress in HepG2 cells[J]. Journal of Jilin Agricultural University: 1−11 [2023-04-06]. DOI: 10.13327/j.jjlau.2020.5510
[16]	李谣, 陈金龙, 夏春燕, 等. 基于HepG2细胞模型的香菇柄粉多酚抗氧化及抗增殖活性性[J]. 食品科学,2016,37(11):190−196. [LI Y, CHEN J L, XIA C Y, et al. Antioxidant and antiproliferative activity of polyphenols in Lentinus edodes stipe powder on HepG2 cells[J]. Food Science,2016,37(11):190−196. doi: 10.7506/spkx1002-6630-201611033 LI Y, CHEN J L, XIA C Y, et al. Antioxidant and antiproliferative activity of polyphenols in Lentinus edodes stipe powder on HepG2 cells[J]. Food Science, 2016, 37(11): 190-196. doi: 10.7506/spkx1002-6630-201611033
[17]	刘先皓, 杨明静, 陈壁, 等. 东紫苏多酚提取物对HepG2细胞氧化损伤的保护作用研究[J]. 食品安全质量检测学报,2021,12(24):9549−9506. [LIU X H, YANG M J, CHEN B, et al. Study on the protective effect of polyphenol extract from Elsholtzia bodinieri Vaniot against oxidative damage of HepG2 Cells[J]. Journal of Food Safety and Quality,2021,12(24):9549−9506. doi: 10.3969/j.issn.2095-0381.2021.24.spaqzljcjs202124031 LIU X H, YANG M J, CHEN B, et al. Study on the protective effect of polyphenol extract from Elsholtzia bodinieri Vaniot against oxidative damage of HepG2 Cells[J]. Journal of Food Safety and Quality, 2021, 12(24): 9549-9506. doi: 10.3969/j.issn.2095-0381.2021.24.spaqzljcjs202124031
[18]	FAROMBI E O, MÖLLER P, DRAGSTED L O. Ex-vivo and in vitro protective effects of kolaviron against oxygen-derived radical-induced DNA damage and oxidative stress in human lymphocytes and rat liver cells[J]. Cell Biology and Toxicology,2004,20(2):71−82. doi: 10.1023/B:CBTO.0000027916.61347.bc
[19]	ZHAO X C, ZHANG L, YU H X, et al. Curcumin protects mouse neuroblastoma neuro-2A cells against hydrogen-peroxide-induced oxidative stress[J]. Food Chemistry,2011,129(2):387−394. doi: 10.1016/j.foodchem.2011.04.089
[20]	朱俊杰. 大青叶抵御HepG2细胞氧化应激损伤及其机制研究[D]. 海口: 海南大学, 2018. ZHU J J. Study on cytoprotective effects and mechanisms of Clerodendrum cyotophyllum Turcz leaves against oxidative stress in HepG2 cells[D]. Haikou: Hainan University, 2018.
[21]	ZHANG Z H, REN Z, CHEN S, et al. ROS generation and JNK activation contribute to 4-methoxy-TEMPO-induced cytotoxicity, autophagy, and DNA damage in HepG2 cells[J]. Archives of Toxicology,2018,92(2):717−728. doi: 10.1007/s00204-017-2084-9
[22]	郭增旺, 樊乃境, 田海芝, 等. 小米糠黄酮对H₂O₂致HepG2氧化应激损伤的保护作用[J]. 食品科学,2020,41(5):159−165. [GUO Z W, FAN N J, TIAN H Z, et al. Protective effects of flavonoids from millet bran on H₂O₂-induced oxidative stress injury in HepG2 cells[J]. Food Science,2020,41(5):159−165. doi: 10.7506/spkx1002-6630-20190101-013 GUO Z W, FAN N J, TIAN H Z, et al. Protective effects of flavonoids from millet bran on H₂O₂-induced oxidative stress injury in HepG2 cells[J]. Food Science, 2020, 41(5): 159-165. doi: 10.7506/spkx1002-6630-20190101-013
[23]	TIAN B, WU Y Y, SHENG D H, et al. Chemiluminescence assay for reactive oxygen species scavenging activities and inhibition on oxidative damage of DNA in Deinococcus radiodurans[J]. Luminescence,2004,19(2):78−84. doi: 10.1002/bio.761
[24]	HSIEHHM, WUWM, HUML. Genistein attenuates D-galactose-induced oxidative damage through decreased reactive oxygen species and NF-κB binding activity in neuronal PC12 cells[J]. Life Sciences,2011,88:82−88. doi: 10.1016/j.lfs.2010.10.021
[25]	BECKMAN J A, GOLDFINE A B, LEOPOLD J A, et al. Ebselen does not improve oxidative stress and vascular function in patients with diabetes: a randomized, crossover trial[J]. American Journal of Physiology, Heart and Circulatory Physiology,2016,311(6):1431−1436. doi: 10.1152/ajpheart.00504.2016
[26]	LIU K, LUO M, WEI S. The bioprotective effects of polyphenols on metabolic syndrome against oxidative stress: Evidences and perspectives[J]. Oxidative Medicine and Cellular Longevity,2019,2019:6713194−6713210.
[27]	YAN F J, ZHENG X D. Anthocyanin-rich mulberry fruit improves insulin resistance and protects hepatocytes against oxidative stress during hyperglycemia by regulating AMPK/ACC/mTOR pathway[J]. Journal of Functional Foods,2017,30:270−281. doi: 10.1016/j.jff.2017.01.027
[28]	XIE J H, YE H D, DU M X, et al. Mung bean protein hydrolysates protect mouse liver cell line Nctc-1469 cell from hydrogen peroxide-induced cell injury[J]. Foods,2020,9(1):14−27.
[29]	张艺, 叶升. 还原型谷胱甘肽生理功能及其临床应用[J]. 生命的化学,2020,40(12):2226−2235. [ZHANG Y, YE S. Physiological functions and clinical applications of glutathione[J]. The Chemistry of Life,2020,40(12):2226−2235. ZHANG Y, YE S. Physiological functions and clinical applications of glutathione[J]. The Chemistry of Life, 2020, 40(12): 2226-2235.
[30]	廖鼐, 龙子, 海春旭, 等. 2, 3, 7, 8-四氯二苯并二噁英染毒或联合高脂饮食致小鼠糖脂代谢紊乱的实验研究[J]. 癌变·畸变·突变,2019,31(2):111−118. [LIAO N, LONG Z, HAI C X, et al. 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin alone or in combination with high fat diet on glucose and lipid metabolism in mice[J]. Carcinogenesis, Aberration, and Mutation,2019,31(2):111−118. LIAO N, LONG Z, HAI C X, et al. 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin alone or in combination with high fat diet on glucose and lipid metabolism in mice[J]. Carcinogenesis, Aberration, and Mutation, 2019, 31(2): 111-118.
[31]	WU J Z, REN J Y, YAO S, et al. Novel antioxidants’ synthesis and their anti-oxidative activity through activating Nrf2 signaling pathway[J]. Bioorganic & Medicinal Chemistry Letters,2017,27(7):1616−1619.
[32]	王敏. 苹果多酚对拘束应激小鼠免疫细胞氧化应激状态的影响[D]. 广州: 暨南大学, 2010. WANG M. Effect of apple polyphenols on immunocyte cells in mice treated with restraint stress[D]. Guangzhou: Jinan university, 2010.
[33]	FRIDOVICH I. Superoxide radical and superoxide dismutases[J]. Annual Review of Biochemistry,1995,64(1):97−112. doi: 10.1146/annurev.bi.64.070195.000525
[34]	李汀, 邹波, 吴继军, 等. 蜜柚果肉膳食多酚的结构鉴定及抗氧化机理[J]. 食品科学,2021,42(19):202−210. [LI T, ZOU B, WU J J, et al. Structural identification and antioxidant mechanism of dietary polyphenols in fruit pulp of honey pomelo[J]. Food Science,2021,42(19):202−210. doi: 10.7506/spkx1002-6630-20201124-238 LI T, ZOU B, WU J J, et al. Structural identification and antioxidant mechanism of dietary polyphenols in fruit pulp of honey pomelo[J]. Food Science, 2021, 42(19): 202-210. doi: 10.7506/spkx1002-6630-20201124-238
[35]	YAO Y J, WANG H L, XU F R, et al. Insoluble-bound polyphenols of adlay seed ameliorate H₂O₂-induced oxidative stress in HepG2 cells via Nrf2 signalling[J]. Food Chemistry, 2020, 325: 126865.

Supplements (0)

Get Citation

PDF

XML

Article Metrics

Article views (179) PDF downloads (12)

Science and Technology of Food Industry

Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP

Abstract

References

Catalog

Article Metrics

Export File

Citation

Format

Content