Preparation of Sulfated Xylans and Its <i>in Vitro</i> Proliferation of Probiotics

Xia LI; Qiying ZHANG; Yuan GUAN; Jing LI; Haishan CHEN; Lifen LI

doi:10.13386/j.issn1002-0306.2022080112

Volume 44 Issue 13

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(13): 134-140. > DOI: 10.13386/j.issn1002-0306.2022080112

LI Xia, ZHANG Qiying, GUAN Yuan, et al. Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics[J]. Science and Technology of Food Industry, 2023, 44(13): 134−140. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080112.

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Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics

1.
College of Chemistry and Bioengineering, Guilin University of Science and Technology, Guilin 541004, China
2.
Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, China

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Received Date: August 09, 2022
Available Online: April 22, 2023

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Abstract

Abstract

This study aimed to determine the effect of sulfated xylans on the growth of probiotics. Xylan (Xyl) was chemically modified using the sulfamic acid-N,N-dimethylformamide method to produce sulfated xylan (SXY). The SXY was characterized by Fourier infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and Congo red experiment. The Xyl and SXY were tested for their in vitro prebiotic effects using four strains of intestinal microflora, including Lactobacillus delbrueckii subsp. bulgaricus (GIM1.155), L. plantarum (GIM1.191), L. brevis (GIM1.773), and Streptococcus thermophilus (GIM1.540). From the recorded infrared spectra, vibrational bands for SXY were observed at 894, 1052 and 1243 cm⁻¹, they could be assigned to the C—O—S vibration of a C—O—SO₃, C—O stretched and S＝O stretching vibrations. The results of scanning electron microscopy showed that the surface structure of SXY increased smoothness. Congo red experiment revealed that SXY had a triple helix structure. The substitution degree of SXY measured using a BaCl₂-gelatin turbidimeter was 0.341. In vitro experiment showed that the optimum concentration of SXY for the in vitro probiotic growth was 2.0%, rapid growth and increased proliferation were observed after 10 h of culture. The results obtained suggest that SXY could promote probiotic growth. It is an alternate source of prebiotics for maintaining gut health.
- xylan (Xyl),
- sulfation,
- probiotics,
- in vitro proliferation,
- prebiotics

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Science and Technology of Food Industry

Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics

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