Milk-Based Extraction Technology Enhances the Bioavailability and Anti-inflammatory Activity of Ginger Extract

MA Zhen; XU Jun; WU Juan; WANG Caiyun; HE Jian; JING Pu

doi:10.13386/j.issn1002-0306.2023020048

Volume 45 Issue 3

Jan. 2024

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Science and Technology of Food Industry > 2024 > 45(3): 310-319. > DOI: 10.13386/j.issn1002-0306.2023020048

MA Zhen, XU Jun, WU Juan, et al. Milk-Based Extraction Technology Enhances the Bioavailability and Anti-inflammatory Activity of Ginger Extract[J]. Science and Technology of Food Industry, 2024, 45(3): 310−319. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020048.

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Milk-Based Extraction Technology Enhances the Bioavailability and Anti-inflammatory Activity of Ginger Extract

MA Zhen^{1, 2,},
XU Jun^{3, 4},
WU Juan^{3, 4},
WANG Caiyun^{3, 5},
HE Jian^{4, 5, ,},
JING Pu^{1, 2, ,}

1.
School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China
2.
Shanghai Jiao Tong University Sichuan Research Institute, Chengdu 610000, China
3.
Inner Mongolia Research Center of Diary Technology Co., Ltd., Hohhot 010080, China
4.
Yili Yinuo Technology (Shanghai) Co., Ltd., Shanghai 201111, China
5.
National Dairy Technology Innovation Center, Hohhot 010080, China

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Received Date: February 06, 2023
Available Online: December 01, 2023

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Abstract

Abstract

This study was conducted to evaluate the bioavailability and anti-inflammatory efficacy of ginger milk extract through cell and animal experiments compared with commercial ginger water extract. High-performance liquid chromatography was used to analyze the contents of ginger phenols in ginger extract. The bioavailability of ginger milk extract in Caco-2 model and its effect on ulcerative colitis in rats were evaluated. The results showed that the content of 6-gingerol, the active component in ginger, was increased by 13.1% in ginger milk extract than that in the water extract. The total phenols in ginger milk extract were more easily absorbed by Caco-2 cells than that in the water extract, increasing by 34.9% after 24 hours of culture. Compared with ginger water extract, ginger milk extract significantly reduced the decline of body mass, DAI score, alleviate colon atropymass, and spleen index of colitis rats. The expression level of TNF-α, IL-1β, IL-6 in colon tissue significantly decreased (P<0.05). The activity of superoxide dismutase (SOD) was increased, and the content of malondialdehyde (MDA) was significantly decreased (P<0.05). The results showed that ginger milk extract showed better bioavailability and effectively relieve the symptoms of ulcerative colitis in rats induced by sodium glucan sulfate. The experimental results could provide theoretical reference for the efficient extraction of ginger active ingredients and the development of functional food.
- ginger milk extract,
- milk-based extraction technology,
- Caco-2 cell absorption,
- ulcerative colitis

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References

[1]	廖怀玉, 韩佳颖, 韩红园, 等. 怀姜的化学成分及其抗炎活性研究[J]. 天然产物研究与开发,2022,34(5):818−823,749. [LIAO H Y, HAN J Y, HAN H Y, et al. Study on chemical constituents and anti-inflammatory activity of Zingiber officinale Roscoe[J]. Natural Product Research and Development,2022,34(5):818−823,749. LIAO H Y, HAN J Y, HAN H Y, et al. Study on chemical constituents and anti-inflammatory activity of Zingiber officinale Roscoe[J]. Natural Product Research and Development, 2022, 34（5）: 818−823,749.
[2]	于宁, 曾虹燕, 邓欣, 等. 姜酚的提取、鉴定及其抗氧化性研究[J]. 食品科学,2007(8):201−204. [YU N, ZENG H Y, DENG X, et al. Study on extraction, identification and antioxidation of gingerol[J]. Food Science,2007(8):201−204. doi: 10.3321/j.issn:1002-6630.2007.08.045 YU N, ZENG H Y, DENG X, et al. Study on extraction, identification and antioxidation of gingerol[J]. Food Science, 2007（8）: 201−204. doi: 10.3321/j.issn:1002-6630.2007.08.045
[3]	刘丹, 张程慧, 安容慧, 等. 生姜主要生物活性成分提取及应用研究进展[J]. 食品工业科技,2016,37(20):391−395, 400. [LIU D, ZHANG C H, AN R H, et al. Advances on extraction and application of ginger bioactive ingredient[J]. Science and Technology of Food Industry,2016,37(20):391−395, 400. doi: 10.13386/j.issn1002-0306.2016.20.070 LIU D, ZHANG C H, AN R H, et al. Advances on extraction and application of ginger bioactive ingredient[J]. Science and Technology of Food Industry, 2016, 37（20）: 391−395, 400. doi: 10.13386/j.issn1002-0306.2016.20.070
[4]	杨崇, 傅若秋, 高宁. 8-姜酚抑制乳腺癌细胞增殖的分子机制研究[J]. 第三军医大学,2019,41(5):406−414. [YANG C, FU R Q, GAO N. Molecular mechanism of inhibition of 8-gingerol on proliferation of breast cancer cells[J]. Journal of Army Medical University,2019,41(5):406−414. YANG C, FU R Q, GAO N. Molecular mechanism of inhibition of 8-gingerol on proliferation of breast cancer cells[J]. Journal of Army Medical University, 2019, 41（5）: 406−414.
[5]	FAROMBI E O, AJAYI B O, ADEDARA I A. 6-Gingerol delays tumorigenesis in benzo [a] pyrene and dextran sulphate sodium-induced colorectal cancer in mice[J]. Food and Chemical Toxicology,2020(142):111483.
[6]	何剑, 陈醇, 徐军, 等. 一种制备药食同源植物提取物的方法:202011360642.4[P]. 2021-06-01. [HE J, CHEN C, XUN J, et al. A method for preparing phytochemical extracts from medicinal and edible plants:202011360642.4[P]. 2021-06-01. HE J, CHEN C, XUN J, et al. A method for preparing phytochemical extracts from medicinal and edible plants: 202011360642.4[P]. 2021-06-01.
[7]	陈海, 马良, 戴宏杰, 等. 铁蛋白纳米笼分子装载途径及食品活性物质递送的研究进展[J]. 食品与发酵工业,2022,48(3):304−310. [CHEN H, MA L, DAI H J, et al. Encapsulation strategies of ferritin and its appliction for food bioactive compounds delivery:A review[J]. Food and Fermentation Industries,2022,48(3):304−310. CHEN H, MA L, DAI H J, et al. Encapsulation strategies of ferritin and its appliction for food bioactive compounds delivery: A review[J]. Food and Fermentation Industries, 2022, 48（3）: 304−310.
[8]	VAN DER BURG-KOOREVAAR M C D, MIRET S, DUCHATEAU G S. Effect of milk and brewing method on black tea catechin bioaccessibility[J]. Journal of Agricultral and Food Chemistry,2011,59(14):7752−7758. doi: 10.1021/jf2015232
[9]	XIE Y, KOSIŃSKA A, XU H, et al. Milk enhances intestinal absorption of green tea catechins in in vitro digestion/Caco-2 cells model[J]. Food Research International,2013,53(2):793−800. doi: 10.1016/j.foodres.2012.07.063
[10]	MA J J, HUANG X N, YIN S W, et al. Bioavailability of quercetin in zein-based colloidal particles-stabilized pickering emulsions investigated by the in vitro digestion coupled with Caco-2 cell monolayer model[J]. Food Chemistry,2021,360:130152. doi: 10.1016/j.foodchem.2021.130152
[11]	LIU J L, GAO Y Y, ZHOU J, et al. Changes in serum inflammatory cytokine levels and intestinal flora in a self-healing dextran sodium sulfate-induced ulcerative colitis murine model[J]. Life Sciences,2020,263:118587. doi: 10.1016/j.lfs.2020.118587
[12]	高锦锦, 郭宇星, 潘道东. Caco-2细胞模型用于乳源ACE抑制肽LL、LPEW的小肠吸收研究[J]. 食品科学,2017,38(11):214−219. [GAO J J, GUO Y X, PAN D D. Intestinal absorption of milk-derived ACE inhibitory peptides LL and LPEW using Caco-2 cell model[J]. Food Science,2017,38(11):214−219. doi: 10.7506/spkx1002-6630-201711034 GAO J J, GUO Y X, PAN D D. Intestinal absorption of milk-derived ACE inhibitory peptides LL and LPEW using Caco-2 cell model[J]. Food Science, 2017, 38（11）: 214−219. doi: 10.7506/spkx1002-6630-201711034
[13]	TIAN W, WANG H, ZHU Y, et al. Intervention effects of delivery vehicles on the therapeutic efficacy of 6-gingerol on colitis[J]. Journal of Controlled Release,2022,349:51−66. doi: 10.1016/j.jconrel.2022.06.058
[14]	ZHANG L L, YAO X F, MA M M, et al. Protective effect of l-theanine against DSS-induced colitis by regulating the lipid metabolism and reducing inflammation via the NF- κB signaling pathway[J]. Journal of Agricultural and Food Chemistry,2021,69(47):14192−14203. doi: 10.1021/acs.jafc.1c05839
[15]	BEST W R, BECKTEL J M, SINGLETON J W, et al. Development of a Crohn disease activity index:National cooperative Crohn disease study[J]. Gastroenterology,1976,70(3):439−444. doi: 10.1016/S0016-5085(76)80163-1
[16]	VRIENS J, APPENDIO G, NILIUS B. Pharmacology of vanilloid transient receptor potential cation channels[J]. Molecular Pharmacology,2009,75(6):1262−1279. doi: 10.1124/mol.109.055624
[17]	LUO R, LIN M, ZHANG C, et al. Genipin-crosslinked human serum albumin coating using a tannic acid layer for enhanced oral administration of curcumin in the treatment of ulcerative colitis[J]. Food Chemistry,2020,330:127241. doi: 10.1016/j.foodchem.2020.127241
[18]	田甜, 李晓晖, 汪晴. 细胞促渗肽促进药物细胞摄取的研究进展[J]. 中国药学杂志,2019,54(16):1285−1291 [TIAN T, LI X H, WANG Q. Development of cell penetrating peptides for enhancing drug absorption[J]. Chinese Pharmaceutical Journal,2019,54(16):1285−1291. TIAN T, LI X H, WANG Q. Development of cell penetrating peptides for enhancing drug absorption[J]. Chinese Pharmaceutical Journal, 2019, 54（16）: 1285−1291.
[19]	寇兴然, 朱松, 马朝阳, 等. 植物多酚生物利用度及提高方法研究进展[J]. 食品与生物技术学报,2017,36(1):1−7. [KOU X R, ZHU S, MA C Y, et al. Research progress of plant polyphenols oral bioavailability and enhancement strategies[J]. Journal of Food Science and Biotechnology,2017,36(1):1−7. doi: 10.3969/j.issn.1673-1689.2017.01.001 KOU X R, ZHU S, MA C Y, et al. Research progress of plant polyphenols oral bioavailability and enhancement strategies[J]. Journal of Food Science and Biotechnology, 2017, 36（1）: 1−7. doi: 10.3969/j.issn.1673-1689.2017.01.001
[20]	石淑亚, 王连生. Ⅱ 相代谢及其酶的研究进展[J]. 中国临床药理学与治疗学,2014,19(1):82−89. [SHI S Y, WANG L S. Progress in research of phase Ⅱ metabolisms and their enzymes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics,2014,19(1):82−89. SHI S Y, WANG L S. Progress in research of phase Ⅱ metabolisms and their enzymes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19（1）: 82−89.
[21]	LIANG N, KIM B J, DALLAS D C. Bioavailability of peptides derived from the in vitro digestion of human milk assessed by Caco-2 cell monolayers[J]. Journal of Agricultural and Food Chemistry,2022,70(23):7077−7084. doi: 10.1021/acs.jafc.2c01246
[22]	蒋玲, 王启明, 饶哲楠, 等. 基于乳液模板法的递送体系:增强生物活性物质稳定性和生物利用度[J]. 食品与发酵工业,2023,49(6):292−299. [JIANG L, WANG Q M, RAO Z N, et al. Delivery system based on emulsion-templated method:Enhancing the stability and bioavailability of bioactive compounds[J]. Food and Fermentation Industries,2023,49(6):292−299. JIANG L, WANG Q M, RAO Z N, et al. Delivery system based on emulsion-templated method: Enhancing the stability and bioavailability of bioactive compounds[J]. Food and Fermentation Industries, 2023, 49（6）: 292−299.
[23]	严媛, 吴伟杰, 郜海燕, 等. 茭白壳提取物对小鼠溃疡性结肠炎的预防作用[J]. 食品科学,2022,43(23):97−105 [YAN Y, WU W J, GAO H Y, et al. Preventive effect of Zizania latifolia shell extract on ulcerative colitis in mice[J]. Food Science,2022,43(23):97−105. YAN Y, WU W J, GAO H Y, et al. Preventive effect of Zizania latifolia shell extract on ulcerative colitis in mice[J]. Food Science, 2022, 43（23）: 97−105.
[24]	张慧莹, 曾丽萍, 任运红, 等. 乳酸芽孢杆菌发酵液护色的山药多糖对葡聚糖硫酸钠诱导的小鼠结肠炎的改善作用及机制[J]. 食品科学,2022,43(9):120−127. [ZHANG H Y, ZENG L P, REN Y H, et al. Ameliorative effect of polysaccharides from Chinese yam treated with the fermentation broth of Bacillus sp. to inhibit browning on dextran sulfate sodium-induced colitis in mice[J]. Food Science,2022,43(9):120−127. ZHANG H Y, ZENG L P, REN Y H, et al. Ameliorative effect of polysaccharides from Chinese yam treated with the fermentation broth of Bacillus sp. to inhibit browning on dextran sulfate sodium-induced colitis in mice[J]. Food Science, 2022, 43（9）: 120−127.
[25]	陈娅娅, 罗非君, 田媛媛, 等. 谷维素抑制DSS诱导的小鼠结肠炎症及其分子机理研究[J]. 现代食品科技,2016,32(5):14−21. [CHEN Y Y, LUO F J, TIAN Y Y, et al. Molecular mechanism underlying the amelioration of dextran sulphate sodium (DSS)-induced colitis in mice by oryzanol[J]. Modern Food Science and Technology,2016,32(5):14−21. doi: 10.13982/j.mfst.1673-9078.2016.5.03 CHEN Y Y, LUO F J, TIAN Y Y, et al. Molecular mechanism underlying the amelioration of dextran sulphate sodium （DSS）-induced colitis in mice by oryzanol[J]. Modern Food Science and Technology, 2016, 32（5）: 14−21. doi: 10.13982/j.mfst.1673-9078.2016.5.03
[26]	FENG X, XIE Q, XU H, et al. Yeast microcapsule mediated natural products delivery for treating ulcerative colitis through anti-inflammatory and regulation of macrophage polarization[J]. ACS Applied Materials & Interfaces,2022,14(27):31085−31098.
[27]	CAO H, LIU J, SHEN P, et al. Protective effect of naringin on DSS-induced ulcerative colitis in mice[J]. Journal of Agricultral and Food Chemistry,2018,66(50):13133−13140. doi: 10.1021/acs.jafc.8b03942
[28]	郝亚楠, 李新平, 刘宁, 等. 薏米提取物对溃疡性结肠炎大鼠抗氧化作用的研究[J]. 中国预防医学杂志,2012,13(3):177−180. [HAO Y N, LI X P, LIU N, et al. Effect of coix lacryma-jobi seed extract on the antioxidation in colities in rats[J]. Chinese Preventive Medicine,2012,13(3):177−180. doi: 10.16506/j.1009-6639.2012.03.011 HAO Y N, LI X P, LIU N, et al. Effect of coix lacryma-jobi seed extract on the antioxidation in colities in rats[J]. Chinese Preventive Medicine, 2012, 13（3）: 177−180. doi: 10.16506/j.1009-6639.2012.03.011
[29]	杨柳青, 董丽, 罗颖华, 等. 氧化应激参与丙烯酰胺致神经细胞凋亡及炎症反应的研究进展[J]. 食品安全质量检测学报,2020,11(18):6265−6270. [YANG L Q, DONG L, LUO Y H, et al. Research progress on the effect of oxidative stress on acrylamide-induced neuronal apoptosis and inflammatory response[J]. Journal of Food Safety and Quality,2020,11(18):6265−6270. doi: 10.19812/j.cnki.jfsq11-5956/ts.2020.18.001 YANG L Q, DONG L, LUO Y H, et al. Research progress on the effect of oxidative stress on acrylamide-induced neuronal apoptosis and inflammatory response[J]. Journal of Food Safety and Quality, 2020, 11（18）: 6265−6270. doi: 10.19812/j.cnki.jfsq11-5956/ts.2020.18.001
[30]	LI Y P, PAN X, YIN M Y, et al. Preventive effect of lycopene in dextran sulfate sodium-induced ulcerative colitis mice through the regulation of TLR4/TRIF/NF- κB signaling pathway and tight junctions[J]. Journal of Agricultural and Food Chemistry,2021,69(45):13500−13509. doi: 10.1021/acs.jafc.1c05128
[31]	ABDELMEGID A M, ABDO F K, AHMED F E, et al. Therapeutic effect of gold nanoparticles on DSS-induced ulcerative colitis in mice with reference to interleukin-17 expression[J]. Scientific Reports,2019,9(1):10176. doi: 10.1038/s41598-019-46671-1

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Science and Technology of Food Industry

Milk-Based Extraction Technology Enhances the Bioavailability and Anti-inflammatory Activity of Ginger Extract

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