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Volume 45 Issue 4
Feb.  2024
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XIE Na, CHENG Junwen, XU Juan, et al. Process Optimization of Enzyme-Assisted Extraction of Polysaccharides from Artificially-Cultivated Cordyceps cicadae and Its Kinetic, Thermodynamic and Antioxidant Activities Analysis[J]. Science and Technology of Food Industry, 2024, 45(4): 151−160. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023040116.
Citation: XIE Na, CHENG Junwen, XU Juan, et al. Process Optimization of Enzyme-Assisted Extraction of Polysaccharides from Artificially-Cultivated Cordyceps cicadae and Its Kinetic, Thermodynamic and Antioxidant Activities Analysis[J]. Science and Technology of Food Industry, 2024, 45(4): 151−160. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023040116.
shu

Process Optimization of Enzyme-Assisted Extraction of Polysaccharides from Artificially-Cultivated Cordyceps cicadae and Its Kinetic, Thermodynamic and Antioxidant Activities Analysis

  • 1.

    Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine,College of Food and Health, Zhejiang A&F University, Hangzhou 311300, China

  • 2.

    Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forestry Resources,Zhejiang Academy of Forestry, Hanghzou 310023, China

  • 3.

    Bioasia Life Institute, Zhejiang Bioasia Pharmaceutical Co., Ltd., Jiaxing 314200, China

  • 4.

    Zhejiang Wuyangtang Pharmaceutical Co., Ltd., Lishui 323000, China

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  • Received Date: March 14, 2023
  • Available Online: December 20, 2023
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    • Abstract
  • Objective: To optimize the enzyme-assisted extraction process of polysaccharides from artificially-cultivated Cordyceps cicadae was conducted in this study. Four different parameters were considered to evaluate the yield of polysaccharides including liquid to solid ratio, enzyme dosage, enzymatic temperature and extraction time. Methods: A four-factor-three-level experimental design was employed to establish a mathematical model by Box-Behnken method, and the scavenging capacity of polysaccharides against three radicals was examined. Afterward, Fick's second law was used to build the kinetic model for the extraction of polysaccharide from artificially-cultivated Cordyceps cicadae. The parameters including rate constants, relative extraction rate, and activation energy were employed to analyze the kinetic and thermodynamic features. Results: Based on the response surface analysis, the optimal extraction process was presented to be as following: Liquid to solid ratio 1:30 g/mL, enzyme dosage 1.6%, enzymatic digestion temperature 67 ℃ and extraction time 90 min. The polysaccharide yield under the above condition was 7.91%, which was close to the predicted value. Moreover, the results of antioxidant capacities indicated that the obtained crude polysaccharides under optimal conditions showed strong DPPH radical scavenging and hydroxyl radical scavenging with IC50 values for 0.60 and 0.54 mg/mL, respectively, and its ORAC value was 45.62 Trolox μmol/g, suggesting potent antioxidant activity in vitro. Conclusion: The study of enzyme-assisted extraction of artificially-cultivated Cordyceps cicadae flower polysaccharides provide theoretical support for the production of polysaccharide fractions from this kind of commercialized Cordyceps cicadae resources.
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