Mechanism of Curcumin Mediated Photodynamic Sterilization on <i>Clostridium perfringens</i>

ZHENG Yuhang; MAI Xutao; WANG Wenzhuo; LIU Fang; ZHANG Xinxiao; SUN Zhilan

doi:10.13386/j.issn1002-0306.2023070251

Volume 45 Issue 12

Jun. 2024

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Science and Technology of Food Industry > 2024 > 45(12): 121-128. > DOI: 10.13386/j.issn1002-0306.2023070251

ZHENG Yuhang, MAI Xutao, WANG Wenzhuo, et al. Mechanism of Curcumin Mediated Photodynamic Sterilization on Clostridium perfringens[J]. Science and Technology of Food Industry, 2024, 45(12): 121−128. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070251.

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Mechanism of Curcumin Mediated Photodynamic Sterilization on Clostridium perfringens

1.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
2.
Institute of Agricultural Products Processing, Agricultural Science, Nanjing 210014, China

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Received Date: July 26, 2023
Available Online: April 18, 2024

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Abstract

Abstract

To achieve the control of foodborne pathogenic bacteria Clostridium perfringens by non-thermal technology, curcumin was used as the photosensitizer to explore its inhibitory mechanism on C. perfringens. The bactericidal effect of different curcumin concentrations on C. perfringens was investigated. The cell morphology after curcumin treatment was investigated through scan electron microscopy. The integrity of the cell membrane was analyzed by confocal laser scanning microscope and leakage of extracellular macromolecules. The oxidative damage of C. perfringens was determined by ROS analysis. The results indicated that curcumin showed a significantly (P<0.05) photodynamic inactivation against C. perfringens. C. perfringens was completely killed when curcumin was used at a concentration of 20 μmol/L for 10 min. Cytotoxic ROS was generated in this process, which caused the damage of DNA and proteins, cell membrane rupture and leakage of cellular contents, ultimately leading to cell death. These findings gave support for the development of photodynamic sterilization technology in low-temperature meat products.
- curcumin,
- photodynamic inactivation,
- Clostridium perfringens,
- antibacterial mechanism,
- oxidative stress

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Mechanism of Curcumin Mediated Photodynamic Sterilization on Clostridium perfringens

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