Effect of Mixed Fermentation on Improving Cottonseed Meal Protein and Reducing Free Gossypol

WEI Jia; LI Li; LIU Jun; MAO Xiang; ZHAO Qikai; ZOU Yuhong

doi:10.13386/j.issn1002-0306.2023100091

Volume 45 Issue 16

Aug. 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(16): 231-238. > DOI: 10.13386/j.issn1002-0306.2023100091

WEI Jia, LI Li, LIU Jun, et al. Effect of Mixed Fermentation on Improving Cottonseed Meal Protein and Reducing Free Gossypol[J]. Science and Technology of Food Industry, 2024, 45(16): 231−238. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100091.

Citation:

PDF (2280 KB)

Effect of Mixed Fermentation on Improving Cottonseed Meal Protein and Reducing Free Gossypol

WEI Jia^1,,
LI Li^1, ,,
LIU Jun¹,
MAO Xiang¹,
ZHAO Qikai^{1, 2},
ZOU Yuhong¹

1.
College of Bioengineering, Sichuan University of Light Chemical Technology, Yibin 644000 , China
2.
Leshan Heng Feng Hua Bang Biotechnology Co., Ltd., Leshan 614000, China

More Information

Received Date: October 12, 2023
Available Online: June 24, 2024

Graphical Abstract

Abstract

Abstract

To reduce the content of free gossypol in cottonseed meal and improve the quality of cottonseed protein, this study used a two-stage fermentation method to treat cottonseed meal. Enterococcus faecalis (E) was first added, and then Aspergillus oryzae (M1) was added for staged fermentation. The fermentation conditions of mixed were optimized by single factor and orthogonal experiments. The results showed that when the inoculation amount of Enterococcus faecium was 1.5%, the fermentation temperature was 37 ℃, the fermentation time was 24 h, the inoculation amount of Aspergillus oryzae was 1.5%, the fermentation temperature was 30 ℃, the fermentation time 96 h, and the initial fermentation moisture of cottonseed meal was 50%, the crude protein content of cottonseed meal increased from 39.6% to 49.64%, the acid-soluble protein content increased from 4.79% to 36.58%, and the free gossypol content decreased from 1370 mg/kg to 45.32 mg/kg, and the detoxification rate reached 96.7%. The results would provide a scientific basis for the high-value utilization of cottonseed meal.
- fermented cottonseed meal,
- two-stage fermentation,
- Enterococcus faecalis,
- Aspergillus oryzae,
- protein quality,
- mixed bacteria fermentation,
- free gossypol

FullText(HTML)

References (26)

References

[1]	中国饲料数据库[EB/OL]. [2020-12-08](2022-10-06)[China Feed Database[EB/OL]. [2020-12-08](2022-10-06.] 2020-12-08]（2022-10-06）[China Feed Database[EB/OL]. [2020-12-08]（2022-10-06.
[2]	潘晶. 棉籽粕蛋白的制备及其性质研究[D]. 无锡:江南大学, 2010. [PAN J, Study on preparation and property of protein from cottonseed meal[D]. Wuxi:Jiangnan University, 2010.] PAN J, Study on preparation and property of protein from cottonseed meal[D]. Wuxi: Jiangnan University, 2010.
[3]	舒文秀, 周瑞, 蓝婧婷, 等. 棉籽粕脱毒技术及其在畜禽生产中的应用[J]. 饲料研究,2021,44(2):147−151. [SHU W X, ZHOU R, LAN J T, et al. Detoxification technology of cottonseed meal and its application in livestock and poultry production[J]. Feed Research,2021,44(2):147−151.] SHU W X, ZHOU R, LAN J T, et al. Detoxification technology of cottonseed meal and its application in livestock and poultry production[J]. Feed Research, 2021, 44（2）: 147−151.
[4]	常磊. 棉粕固态发酵及游离棉酚降解的研究[D]. 广州:暨南大学, 2013. [CHANG L, Study on the solid-state fermentation of cottonseed meal and the degradation of free gossypol[D]. Guangzhou:Jinan University, 2013.] CHANG L, Study on the solid-state fermentation of cottonseed meal and the degradation of free gossypol[D]. Guangzhou: Jinan University, 2013.
[5]	HUANG W Q, CUI K, HAN Y, et al. Long term effects of artificial rearing before weaning on the growth performance, ruminal microbiota and fermentation of fattening lambs[J]. Journal of Integrative Agriculture,2022,21(4):1146−1160.
[6]	RONBINSON P H, GETACHEW G, PETERS E J D, et al. Influence of variety and storage for up to 22 days on nutrient composition and gossypol level of Pima cottonseed (Gossypium spp.)[J]. Animal Feed Science & Technology,2001,91(3−4):149−156.
[7]	GADELHA I, FONSECA N, OLORIS S, et al. Gossypol toxicity from cottonseed products[J]. The Scientific World Journal,2014,20(2):231635.
[8]	倪海球, 李军国, 杨玉娟, 等. 响应面分析法优化棉粕膨化工艺参数[J]. 饲料工业, 2020, 41(21):24−31. [NI H Q, LI J G, YANG Y J, et al, Response surface analysis method for optimizing cotton meal puffing process parameters[J]. Feed Industry, 2020, 41(21):24−31.] NI H Q, LI J G, YANG Y J, et al, Response surface analysis method for optimizing cotton meal puffing process parameters[J]. Feed Industry, 2020, 41（21）: 24−31.
[9]	MA X K, HU J X, SHANG Q, et al. Chemical composition, energy content and amino acid digestibility in cottonseed meals fed to growing pigs[J]. Chemical composition, energy content and amino acid digestibility in,2019,47(1):20−28.
[10]	王丽, 王薇薇, 周航, 等. 棉粕脱毒工艺及游离棉酚对蛋鸡性能影响的研究进展[J]. 粮油食品科技, 2019, 27(2):50−54. [WANG L, WANG W W, ZHOU H, et al, Research progress on detoxification of gossypol in cottonseed meal and the effects of free gossypol on the performance of laying hens[J]. Science and Technology of Cereals, Oils and Foods, 2019, 27(2):50−54.] WANG L, WANG W W, ZHOU H, et al, Research progress on detoxification of gossypol in cottonseed meal and the effects of free gossypol on the performance of laying hens[J]. Science and Technology of Cereals, Oils and Foods, 2019, 27（2）: 50−54.
[11]	张莉, 杨晓龙, 王海亮, 等. 一株棉酚降解菌株的筛选鉴定及对棉粕的脱毒效果[J]. 新疆农业科学, 2022, 59(12):3057−3065. [ZHANG L, YANG X L, WANG H L, et al, Selection and identification of a cotton phenol degradation strain and its detoxification effect on cotton meal[J]. Xinjiang Agricultural Sciences, 2022, 59(12):3057−3065.] ZHANG L, YANG X L, WANG H L, et al, Selection and identification of a cotton phenol degradation strain and its detoxification effect on cotton meal[J]. Xinjiang Agricultural Sciences, 2022, 59（12）: 3057−3065.
[12]	吴慧昊, 舒文秀, 李姿茹, 等. 游离棉酚脱除菌株的分离鉴定及体内定植效果研究[J]. 中国酿造, 2022, 41(3):104−109. [WU H H, SHU W X, LI Z R, et al, lsolation, identification and in vivo colonization of free gossypol-degrading strain[J]. China Brewing, 2022, 41(3):104−109.] WU H H, SHU W X, LI Z R, et al, lsolation, identification and in vivo colonization of free gossypol-degrading strain[J]. China Brewing, 2022, 41（3）: 104−109.
[13]	宣秋希, 乔琳, 侯晓林, 等. 固态生料发酵棉籽粕菌种筛选及发酵工艺的研究[J]. 动物营养学报,2022,34(5):3376−3391. [XUAN Q X, QIAO L, HOU X L, et al. Screening of strains for solid-state fermentation of raw cottonseed meal and study on fermentation technology[J]. Chinese Journal of Animal Nutrition,2022,34(5):3376−3391.] doi: 10.3969/j.issn.1006-267x.2022.05.062 XUAN Q X, QIAO L, HOU X L, et al. Screening of strains for solid-state fermentation of raw cottonseed meal and study on fermentation technology[J]. Chinese Journal of Animal Nutrition, 2022, 34（5）: 3376−3391. doi: 10.3969/j.issn.1006-267x.2022.05.062
[14]	国家质量监督检验检疫总局. GB/T21264-2007饲料用棉籽粕[S]. 北京:中国标准出版社, 2017. [General Administration of Quality Supervision, lnspection and Quarantine of the People’s Republic of China GB/T21264-2007 Cottonseed meal(solvent)for feedstuffs[S] Beijing:China Standards Publishing House, 2017:12−24.] General Administration of Quality Supervision, lnspection and Quarantine of the People’s Republic of China GB/T21264-2007 Cottonseed meal（solvent）for feedstuffs[S] Beijing: China Standards Publishing House, 2017: 12−24.
[15]	国家市场监督管理总局. GB/T 13086-2020饲料中游离棉酚的测定方法[S]. 北京:中国标准出版社, 2020. [State Administration for Market Regulation GB/T 13086-2020 Method for determination of free gossypol in feeds[S]Beijing:China Standards Publishing House, 2020.] State Administration for Market Regulation GB/T 13086-2020 Method for determination of free gossypol in feeds[S]Beijing: China Standards Publishing House, 2020.
[16]	农业农村部. NY/T 3801-2020 饲料原料中酸溶蛋白的测定[S]. 北京:中国标准出版社, 2020:11−12. [Ministry of agriculture and rural affairs NY/T 3801-2020 Determination of acid-soluble protein in feed materials[S]Beijing:China Standards Publishing House, 2020:11−12.] Ministry of agriculture and rural affairs NY/T 3801-2020 Determination of acid-soluble protein in feed materials[S]Beijing: China Standards Publishing House, 2020: 11−12.
[17]	MAHARACH M, METHA W. Phytonutrient pellet supplementation enhanced rumen fermentation efficiency and milk production of lactating Holstein-Friesian crossbred cows[J]. Animal Nutrition,2022,9(2):119−126.
[18]	SON H, TANG J W, YAO X H, et al. Improvement of the nutritional quality of cottonseed meal by Bacillus subtilis and the addition of papain[J]. Food Science and Technology,2012,14(4):563−568.
[19]	李园园. 米曲霉产淀粉酶和纤维素酶的初步研究[D]. 天津:天津科技大学, 2014. [LI Y Y. Primary study on the amylase and cellulase fermented in Aspergillus oryzae[D]. Tianjin:Tianjin University of Science and Technology, 2014.] LI Y Y. Primary study on the amylase and cellulase fermented in Aspergillus oryzae[D]. Tianjin: Tianjin University of Science and Technology, 2014.
[20]	管浩. 外源氮和锰对黑曲霉降解小麦秸秆的影响[D]. 合肥:安徽农业大学, 2023. [GUAN H. Effects of exogenous nitrogen and manganese on decomposition of wheat straw by Aspergillus niger[D]. Hefei:Anhui Agricultural University, 2023.] GUAN H. Effects of exogenous nitrogen and manganese on decomposition of wheat straw by Aspergillus niger[D]. Hefei: Anhui Agricultural University, 2023.
[21]	王晓玲, 刘倩, 韩伟, 等. 棉酚脱除菌株的筛选及棉粕混菌固态发酵研究[J]. 粮油食品科技,2016,24(1):81−85. [WANG X L, LIU Q, HAN W, et al. Screening of gossypol-removing strain and the fermentation of cottonseed meal with mixed culture[J]. Science and Technology of Cereals, Oils and Foods,2016,24(1):81−85.] doi: 10.3969/j.issn.1007-7561.2016.01.019 WANG X L, LIU Q, HAN W, et al. Screening of gossypol-removing strain and the fermentation of cottonseed meal with mixed culture[J]. Science and Technology of Cereals, Oils and Foods, 2016, 24（1）: 81−85. doi: 10.3969/j.issn.1007-7561.2016.01.019
[22]	洪振. 微生物固态发酵法降低棉粕中游离棉酚含量的研究[D]. 武汉:武汉轻工大学, 2017. [HONG Z. Microbial solid fermentation to reduce free gossypol content in the cottonseed meal[D]. Wuhan:Wuhan Polytechnic University, 2017.] HONG Z. Microbial solid fermentation to reduce free gossypol content in the cottonseed meal[D]. Wuhan: Wuhan Polytechnic University, 2017.
[23]	ZHANG W J, XU Z R, SUN J Y, et al. Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal[J]. Journal of Zhejiang University Science(Life Science),2006(9):690−695.
[24]	亢乐. 基于比较组学研究豆类发酵食品制曲过程米曲霉关键蛋白酶[D]. 无锡:江南大学, 2023. [KANG L. Study on the key proteases of Aspergillus oryzae during koji-making of bean-based fermented food based on comparative omics analysis[D] Jiangnan University, 2023.] KANG L. Study on the key proteases of Aspergillus oryzae during koji-making of bean-based fermented food based on comparative omics analysis[D] Jiangnan University, 2023.
[25]	BAHAR M, BERNA N, et al. Organic acids production from low-cost cellulosic substrates by fermentation[J]. Biomass Conversion and Biorefinery,2023,28(4):146−148.
[26]	唐金泉, 宋卫国, 黄隽辉. 棉籽精深加工新技术与新工艺[J]. 中国油脂,2007(10):32−35. [TANG J Q, SONG W G, HUANG J H. New technology and process for cotton seed deep processing[J]. China Oils and Fats,2007(10):32−35.] doi: 10.3321/j.issn:1003-7969.2007.10.008 TANG J Q, SONG W G, HUANG J H. New technology and process for cotton seed deep processing[J]. China Oils and Fats, 2007（10）: 32−35. doi: 10.3321/j.issn:1003-7969.2007.10.008

Supplements (1)

Supplements
Other Related Supplements

Get Citation

PDF

XML

Article Metrics

Article views (110) PDF downloads (15)

Science and Technology of Food Industry

Effect of Mixed Fermentation on Improving Cottonseed Meal Protein and Reducing Free Gossypol

Abstract

References

Supplements

Other Related Supplements

Catalog

Article Metrics

Export File

Citation

Format

Content