Effects of Low-carbon and High Protein Bread Diet on Blood Lipid, Immune Index and Intestinal Flora in Mice

JIANG Yuchun; XIAO Yuhang; LI Dajun

doi:10.13386/j.issn1002-0306.2023120097

Volume 45 Issue 20

Oct. 2024

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Science and Technology of Food Industry > 2024 > 45(20): 341-348. > DOI: 10.13386/j.issn1002-0306.2023120097

JIANG Yuchun, XIAO Yuhang, LI Dajun. Effects of Low-carbon and High Protein Bread Diet on Blood Lipid, Immune Index and Intestinal Flora in Mice[J]. Science and Technology of Food Industry, 2024, 45(20): 341−348. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120097.

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Effects of Low-carbon and High Protein Bread Diet on Blood Lipid, Immune Index and Intestinal Flora in Mice

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China

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Received Date: December 10, 2023
Available Online: August 13, 2024

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Objective: To observe the effects of low carbohydrate and high protein diet (LC-HP) and high oil and high sugar diet on blood lipid, immune index and intestinal flora in mice. Use of high protein gluten as the main ingredient to make bread as the diet of mice. Methods: Healthy Kunming mice were randomly divided into low-carbon and high-protein group (group A, CHO 11.41%, Pr 39.18%), control group (group B, CHO 47.4%, Pr 9.6%), high-oil group (group C, CHO 51.4%, Pr 8.5%) and high-sugar group (group D, CHO 60.6%, Pr 7.3%). Each group consisted of 10 animals, 50 parts male and 50 parts female, for a 28 d experimental period. The mice were weighed every 7 days, and the organ coefficient was determined at the end of the experiment. The serum triglyceride (TG), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (high density lipoprotein cholesterol, HDL-C), tumor necrosis factor-α (TNF-α) and serum interleukin-6 (IL-6) were detected. 16S rDNA sequencing was used to detect the changes of flora in mouse fecal samples. Results: The weight growth rate of mice in group A was significantly lower than that in groups C and D (P<0.05). The levels of total TG and TC in group A were significantly lower than those in the other three groups (P<0.05), the levels of LDL-C in group A were significantly lower than those in groups C and D (P<0.05), and the levels of HDL-C in group A were significantly higher than those in group D (P<0.05). The immune organ coefficient of group A was higher than that of the other three groups, but there was no significant difference. The serum levels of TNF-α and IL-6 in group A were significantly higher than those in groups C and D (P<0.05). ACE, Chao1 and Shannon index of group A, which reflected the richness and diversity of intestinal microbial communities, were higher than those of the other three groups, and the relative abundance of beneficial bacteria was significantly increased (P<0.05). Conclusion: Compared with ordinary bread and high-sugar and high-oil bread, eating low-carbon water bread in mice could reduce the weight growth rate and blood lipid level, adjust immune indexes and improve the composition of intestinal flora, and it is confirmed that high-sugar bread had greater adverse health effects than high-oil bread.
- low-carbon water and high-protein diet,
- blood lipid,
- immunity,
- flora

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References

[1]	林旭, 刘鑫, 黎怀星, 等. 肥胖的膳食控制策略[J]. 内科理论与实践,2017,12(4):245−255. [LIN Xu, LIU Xin, LI Huaixing, et al. Diet control strategies for obesity[J]. Journal of Internal Medicine Concepts & Practice,2017,12(4):245−255.] LIN Xu, LIU Xin, LI Huaixing, et al. Diet control strategies for obesity[J]. Journal of Internal Medicine Concepts & Practice, 2017, 12（4）: 245−255.
[2]	KIRKPATRICK C F, BOLICK J P, KRIS-ETHERTON P M, et al. Review of current evidence and clinical recommendations on the effects of low-carbohydrate and very-low-carbohydrate (including ketogenic) diets for the management of body weight and other cardiometabolic risk factors:A scientific statement from the national lipid association nutrition and lifestyle task force[J]. Journal of Clinical Lipidology,2019,13(5):689−711. doi: 10.1016/j.jacl.2019.08.003
[3]	WOJCIK J L, AUKEMA H M, ZAHRADKA P, et al. Effects of high protein diets on metabolic syndrome parameters[J]. Current Opinion in Food Science,2016,8:43−49. doi: 10.1016/j.cofs.2016.02.001
[4]	GAESSER G A, MILLER JONES J, ANGADI S S. Perspective:Does glycemic index matter for weight loss and obesity prevention? examination of the evidence on “fast” compared with “slow” carbs[J]. Advances in Nutrition,2021,12(6):2076−2084. doi: 10.1093/advances/nmab093
[5]	SCHUTTE S, ESSER D, SIEBELINK E, et al. Diverging metabolic effects of 2 energy-restricted diets differing in nutrient quality:A 12-week randomized controlled trial in subjects with abdominal obesity[J]. The American Journal of Clinical Nutrition,2022,116(1):132−150. doi: 10.1093/ajcn/nqac025
[6]	BASILE A, RENNER M, SCILLIAN J, et al. Restricting calories on low-carbohydrate vs low-fat diets for weight loss:A systematic review and meta-analysis[J]. Current Developments in Nutrition,2020,4:nzaa063_007. doi: 10.1093/cdn/nzaa063_007
[7]	MANCINI J G, FILION K B, ATALLAH R, et al. Systematic review of the mediterranean diet for long-term weight loss[J]. The American Journal of Medicine,2016,129(4):407−415. doi: 10.1016/j.amjmed.2015.11.028
[8]	张明. 低碳水化合物饮食在糖尿病中的应用[J]. 糖尿病天地(临床),2015,9(2):83. [ZHANG Ming. Application of low-carbohydrate diet in diabetes[J]. Clinical Journal of Diabetes World (Clinical),2015,9(2):83.] ZHANG Ming. Application of low-carbohydrate diet in diabetes[J]. Clinical Journal of Diabetes World （Clinical）, 2015, 9（2）: 83.
[9]	卫星, 孙萍. 高蛋白饮食体重管理模式对超重/肥胖病人代谢指标的影响[J]. 中西医结合心脑血管病杂志,2023,21(20):3840−3843. [WEI Xing, SUN Ping. Effect of high-protein diet and weight management model on metabolic indexes of overweight/obese patients[J]. Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease,2023,21(20):3840−3843.] doi: 10.12102/j.issn.1672-1349.2023.20.032 WEI Xing, SUN Ping. Effect of high-protein diet and weight management model on metabolic indexes of overweight/obese patients[J]. Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease, 2023, 21（20）: 3840−3843. doi: 10.12102/j.issn.1672-1349.2023.20.032
[10]	KOSTOGRYS R B, FRANCZYK-ŻARÓW M, MAŚLAK E, et al. Effect of low carbohydrate high protein (LCHP) diet on lipid metabolism, liver and kidney function in rats[J]. Environmental Toxicology and Pharmacology,2015,39(2):713−719. doi: 10.1016/j.etap.2015.01.008
[11]	XIN Z, YU Z, TIANYI Z, et al. Potential of hydrolyzed wheat protein in soy-based meat analogues:Rheological, textural and functional properties[J]. Food Chemistry:X, 2023:20100921.
[12]	王泽宇. 谷朊粉应用及深加工技术探讨[J]. 食品安全导刊,2019(36):71. [WANG Zeyu. Discussion on the application and deep processing technology of gluten[J]. China Food Safety Magazine,2019(36):71.] WANG Zeyu. Discussion on the application and deep processing technology of gluten[J]. China Food Safety Magazine, 2019（36）: 71.
[13]	吕一鸣, 田潇凌, 王晓曦, 等. 小麦蛋白质研究与开发现状[J]. 粮食加工,2022,47(3):8−13. [LÜ Yiming, TIAN Xiaoling, WANG Xiaoxi, et al. Research and development status of wheat protein[J]. Grain Processing,2022,47(3):8−13.] doi: 10.3969/j.issn.1007-6395.2022.3.xblykj202203004 LÜ Yiming, TIAN Xiaoling, WANG Xiaoxi, et al. Research and development status of wheat protein[J]. Grain Processing, 2022, 47（3）: 8−13. doi: 10.3969/j.issn.1007-6395.2022.3.xblykj202203004
[14]	戴媛, 冷进松, 傅婷婷. 豌豆蛋白面包的制作工艺优化及其品质[J]. 食品工业科技,2020,41(11):194−199. [DAI Yuan, LENG Jinsong, FU Tingting. Processing optimization and quality of pea protein bread[J]. Science and Technology of Food Industry,2020,41(11):194−199.] DAI Yuan, LENG Jinsong, FU Tingting. Processing optimization and quality of pea protein bread[J]. Science and Technology of Food Industry, 2020, 41（11）: 194−199.
[15]	国家食品药品监督管理总局. GB 5009.5-2016 食品安全国家标准食品中蛋白质的测定[S]. 北京:中国标准出版社. China Food and Drug Administration. GB 5009.5-2016 National food safety standards. Determination of protein in Food[S]. Beijing:Standards Press of China.
[16]	李小彦, 姜晓燕, 刘美娟. 食品中碳水化合物计算方法探讨[J]. [J]. 现代食品,2020(10):175−176,179. [LI Xiaoyan, JIANG Xiaoyan, LIU Meijuan. Discussion on calculation method of carbohydrate in food[J]. Modern Food,2020(10):175−176,179.] LI Xiaoyan, JIANG Xiaoyan, LIU Meijuan. Discussion on calculation method of carbohydrate in food[J]. Modern Food, 2020（10）: 175−176,179.
[17]	LIU Y, YE L, CHEN H, et al. Herbicide propisochlor exposure induces intestinal barrier impairment, microbiota dysbiosis and gut pyroptosis[J]. Ecotoxicology and Environmental Safety,2023,262:115154. doi: 10.1016/j.ecoenv.2023.115154
[18]	李志春, 陈赶林, 郑凤锦, 等. 甘蔗醋对高脂喂养小鼠体质量、脏器系数和血清生化指标的影响[J]. 中国酿造,2020,39(1):50−54. [LI Zhichun, CHEN Ganlin, ZHENG Fengjin, et al. Effects of sugarcane vinegar on body weight, organ coefficient and serum biochemical indexes in mice fed with high fat[J]. China Brewing,2020,39(1):50−54.] doi: 10.11882/j.issn.0254-5071.2020.01.010 LI Zhichun, CHEN Ganlin, ZHENG Fengjin, et al. Effects of sugarcane vinegar on body weight, organ coefficient and serum biochemical indexes in mice fed with high fat[J]. China Brewing, 2020, 39（1）: 50−54. doi: 10.11882/j.issn.0254-5071.2020.01.010
[19]	杨玲, 陈可纯, 罗朵生, 等. 基于肠道菌群-TβMCA-FXR轴探讨田黄方对老年脂代谢紊乱小鼠作用机制[J]. 中药药理与临床,2023,39(1):18−24. [YANG Ling, CHEN Kechun, LUO Duosheng, et al. Based on the intestinal flora -TβMCA-FXR axis, the mechanism of Tianhuangfang in the treatment of senile mice with lipid metabolism disorder[J]. Pharmacology and Clinics of Chinese Materia Medica,2023,39(1):18−24.] YANG Ling, CHEN Kechun, LUO Duosheng, et al. Based on the intestinal flora -TβMCA-FXR axis, the mechanism of Tianhuangfang in the treatment of senile mice with lipid metabolism disorder[J]. Pharmacology and Clinics of Chinese Materia Medica, 2023, 39（1）: 18−24.
[20]	杨国平, 叶敏霞, 张荣, 等. 基于高通量测序的鼠类肠道细菌群落多样性分析[J]. 中华卫生杀虫药械,2021,27(2):148−153. [YANG Guoping, YE Minxia, ZHANG Rong, et al. Diversity analysis of intestinal bacterial community in rodents based on high-throughput sequencing[J]. Chinese Journal of Hygienic Insecticides & Equipments,2021,27(2):148−153.] YANG Guoping, YE Minxia, ZHANG Rong, et al. Diversity analysis of intestinal bacterial community in rodents based on high-throughput sequencing[J]. Chinese Journal of Hygienic Insecticides & Equipments, 2021, 27（2）: 148−153.
[21]	赖碧玉, 洪梦颖, 何永嘉, 等. 基于16S rDNA技术探讨针灸对腹泻型肠易激综合征模型大鼠肠道菌群的影响[J]. 中国针灸,2023,43(12):1411−1421. [LAI Biyu, HONG Mengying, HE Yongjia, et al. Based on 16S rDNA technology, this paper discusses the influence of acupuncture on intestinal flora in rats with diarrhea-predominant irritable bowel syndrome[J]. Chinese Acupuncture & Moxibustion,2023,43(12):1411−1421.] LAI Biyu, HONG Mengying, HE Yongjia, et al. Based on 16S rDNA technology, this paper discusses the influence of acupuncture on intestinal flora in rats with diarrhea-predominant irritable bowel syndrome[J]. Chinese Acupuncture & Moxibustion, 2023, 43（12）: 1411−1421.
[22]	中国营养学会. 中国营养学会发布《2023版中国居民膳食营养素参考摄入量》[J]. 营养学报, 2023, 45(5):414. [China Nutrition Society. China Nutrition Society issued the Reference Intake of Dietary Nutrients for China Residents[J]. Journal of Nutrition, 2023, 45(5):414.] China Nutrition Society. China Nutrition Society issued the Reference Intake of Dietary Nutrients for China Residents[J]. Journal of Nutrition, 2023, 45（5）: 414.
[23]	方婷, 徐馨, 曲梦影, 等. 代糖与健康的研究进展[J]. 环境与职业医学,2023,40(7):775−781. [FANG Ting, XU Xin, QU Mengying, et al. Research progress of sugar substitute and health[J]. Journal of Environmental and Occupational Medicine,2023,40(7):775−781.] doi: 10.11836/JEOM22468 FANG Ting, XU Xin, QU Mengying, et al. Research progress of sugar substitute and health[J]. Journal of Environmental and Occupational Medicine, 2023, 40（7）: 775−781. doi: 10.11836/JEOM22468
[24]	CARLSON J L, ERICKSON J M, LLOYD B B, et al. Health effects and sources of prebiotic dietary fiber[J]. Current Developments in Nutrition,2018,2(3):nzy005. doi: 10.1093/cdn/nzy005
[25]	刘爽, 彭依晴, 杜晨阳, 等. 影响饱腹感的成分及其生理功能的研究进展[J]. 食品安全质量检测学报,2021,12(22):8827−8833. [LIU Shuang, PENG Yiqing, DU Chenyang, et al. Research progress on components affecting satiety and their physiological functions[J]. Journal of Food Safety & Quality,2021,12(22):8827−8833.] LIU Shuang, PENG Yiqing, DU Chenyang, et al. Research progress on components affecting satiety and their physiological functions[J]. Journal of Food Safety & Quality, 2021, 12（22）: 8827−8833.
[26]	曾江琴, 孙勤国, 徐鸿婕, 等. 小陷胸汤对高血脂小鼠血脂及免疫功能的影响[J]. 现代免疫学,2021,41(5):374−379. [ZENG Jiangqin, SUN Qinguo, XU Hongjie, et al. Effect of Xiaoxinxiong decoction on blood lipid and immune function in hyperlipidemia mice[J]. Current Immunology,2021,41(5):374−379.] ZENG Jiangqin, SUN Qinguo, XU Hongjie, et al. Effect of Xiaoxinxiong decoction on blood lipid and immune function in hyperlipidemia mice[J]. Current Immunology, 2021, 41（5）: 374−379.
[27]	LIN S, YU Y, CENTA M, et al. Induction of lipid-lowering immune reactions by apolipoprotein B-specific T-helper cells in hypercholesterolemic mice[J]. Atherosclerosis,2023,379:S11.
[28]	任彩君, 吴黎明, 王凯. 膳食多酚对肠道菌群影响研究进展[J]. 食品工业科技,2022,43(1):400−409. [REN Caijun, WU Liming, WANG Kai. Research progress on the effect of dietary polyphenols on intestinal flora[J]. Science and Technology of Food Industry,2022,43(1):400−409.] REN Caijun, WU Liming, WANG Kai. Research progress on the effect of dietary polyphenols on intestinal flora[J]. Science and Technology of Food Industry, 2022, 43（1）: 400−409.
[29]	YAO Y, CAI X, FEI W, et al. The role of short-chain fatty acids in immunity, inflammation and metabolism[J]. Crit Rev Food Sci Nutr,2022,62(1):1−12. doi: 10.1080/10408398.2020.1854675
[30]	汪雪, 陈颖, 崔省委, 等. 乳杆菌属益生菌在家畜疾病防治中的应用[J]. 甘肃畜牧兽医,2022,52(6):17−20,29. [WANG Xue, CHEN Ying, CUI Shengwei, et al. Application of Lactobacillus probiotics in prevention and treatment of livestock diseases[J]. Gansu Animal Husbandry and Veterinary Medicine,2022,52(6):17−20,29.] doi: 10.3969/j.issn.1006-799X.2022.06.006 WANG Xue, CHEN Ying, CUI Shengwei, et al. Application of Lactobacillus probiotics in prevention and treatment of livestock diseases[J]. Gansu Animal Husbandry and Veterinary Medicine, 2022, 52（6）: 17−20,29. doi: 10.3969/j.issn.1006-799X.2022.06.006

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Effects of Low-carbon and High Protein Bread Diet on Blood Lipid, Immune Index and Intestinal Flora in Mice

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