Simultaneously Detecting of 13 Illegally Added Drugs in Health Foods for Improving Sexual and Prostate Function by High Performance Liquid Chromatography

LI Rong; ZHU Bin; GENG Zhao; YAN Yujun; LIU Longxing; ZHANG Wenbo

doi:10.13386/j.issn1002-0306.2023120148

Volume 45 Issue 22

Nov. 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(22): 236-243. > DOI: 10.13386/j.issn1002-0306.2023120148

LI Rong, ZHU Bin, GENG Zhao, et al. Simultaneously Detecting of 13 Illegally Added Drugs in Health Foods for Improving Sexual and Prostate Function by High Performance Liquid Chromatography[J]. Science and Technology of Food Industry, 2024, 45(22): 236−243. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120148.

Citation:

PDF (1841 KB)

Simultaneously Detecting of 13 Illegally Added Drugs in Health Foods for Improving Sexual and Prostate Function by High Performance Liquid Chromatography

1.
Bazhong Product Inspection and Testing Center, Bazhong 636000, China
2.
Sichuan Institute for Drug Control (SichuanTesting Center of Medical Devices)/NMPA Key Laboratory for Quality Evaluation for Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Chengdu 611731, China)

More Information

Received Date: December 13, 2023
Available Online: September 13, 2024

Graphical Abstract

Abstract

Abstract

To simultaneously detect 13 illegally added chemical drugs, a high performance liquid chromatography (HPLC) analytical method applicable to health food products for improving sexual function and prostate function was established. The samples were processed by vortex shaking for 1 min, followed by methanol extraction for 10 min under the ultrasonic condition. Then, the sample was separated on an Agilent 5 HC-C₁₈ (2) column (250 mm×4.6 mm, 5 µm) with a gradient separation using methanol-phosphate-triethylamine buffer as the mobile phase. The detection wavelengths were of 218 nm and 245 nm, the column temperature was 30 ℃, and the sample size was 10 µL. Results showed that the correlation coefficients (r) in the linear range were all greater than 0.999, the detection limits were 0.76～4.61 mg/kg, the average recoveries were 92.4%~100.7%, and the relative standard deviations were 0.44%~1.06%. The method was applied to the rapid screening of 65 batches of health foods for improving sexual and prostate function, and results indicated that paroxetine hydrochloride was detected in 2 batches of samples (15.26 mg/tablet, 6.34 mg/pellet, respectively) and yohimbine hydrochloride was detected in 1 batch of samples (10.12 mg/pellet). In summary, the method is easy to conduct and characterized with high sensitivity and reliability, which is suitable for daily supervision and inspection of illegal added drugs in health foods for improving sexual and prostate function.
- high performance liquid chromatography,
- sexual improvement,
- prostate improvement,
- health food,
- illegal addition

FullText(HTML)

References (36)

References

[1]	MA M H, ZHANG J N, MA X L, et al. Using UHPLC-HRMS-based comprehensive strategy to efficiently and accurately screen and identify illegal additives in health-care foods[J]. Food Research International,2023,170:110315.
[2]	BRACCI E L, DAVIS C R, MURPHY K J. Developing a mediterranean healthy food basket and an updated australian healthy food basket modelled on the australian guide to healthy eating[J]. Nutrients,2023,15(7):1692. doi: 10.3390/nu15071692
[3]	余晓琴, 刘美, 李澍才, 等. 新型食品中非法添加挖掘及应对策略研究[J]. 中国食品卫生杂志,2023,35(9):1357−1363. [YU X Q, LIU M, LI S C, et al. Research on mining hidden rules of new unconventional illegal food addition andcoping strategies[J]. Chinese Journal of Food Hygiene,2023,35(9):1357−1363.] YU X Q, LIU M, LI S C, et al. Research on mining hidden rules of new unconventional illegal food addition andcoping strategies[J]. Chinese Journal of Food Hygiene, 2023, 35（9）: 1357−1363.
[4]	陈东洋, 张昊, 冯家力, 等. 保健食品中违禁药物检验技术研究进展[J]. 色谱,2020,38(8):880−890. [CHEN D Y, ZHANG H, FENG J L, et al. Advances in technologies for determination of illegal drugs in health food[J]. Chinese Journal of Chromatography,2020,38(8):880−890.] CHEN D Y, ZHANG H, FENG J L, et al. Advances in technologies for determination of illegal drugs in health food[J]. Chinese Journal of Chromatography, 2020, 38（8）: 880−890.
[5]	胡青, 孙健, 季申. 中药和食品中非法添加化学药品新趋势及监管对策[J]. 中国食品药品监管,2022(3):88−95. [HU Q, SUN J, JI S. Illegally added chemicals in traditional Chinese medicines and foods:New trends and regulatory countermeasures[J]. China Food & Drug Administration Magazine,2022(3):88−95.] HU Q, SUN J, JI S. Illegally added chemicals in traditional Chinese medicines and foods: New trends and regulatory countermeasures[J]. China Food & Drug Administration Magazine, 2022（3）: 88−95.
[6]	徐硕, 金鹏飞, 徐文峰, 等. HPLC-DAD法检测补肾壮阳和改善前列腺功能保健品中非法添加的17种化学药物[J]. 中国药师,2022,25(6):1108−1114. [XU S, JIN P F, XU W F, et al. Determination of seventeen chemical drugs illegally adulterated in health foods for invigorating kidney-yang and improving prostatic function by HPLC-DAD[J]. China Pharmacist,2022,25(6):1108−1114.] XU S, JIN P F, XU W F, et al. Determination of seventeen chemical drugs illegally adulterated in health foods for invigorating kidney-yang and improving prostatic function by HPLC-DAD[J]. China Pharmacist, 2022, 25（6）: 1108−1114.
[7]	ZHANG C F, LI C L, GENG Q, et al. Reasons and treatment strategy for discontinuation of dapoxetine treatment in premature ejaculation patients in China:A retrospective observational study[J]. Andrologia,2022,54(7):14425.
[8]	LELIEFELD H H J, DEBRUYNE F M J, REISMAN Y. The post-finasteride syndrome:Possible etiological mechanisms and symptoms[J]. International Journal of Impotence Research, 2023: 37697052.
[9]	VARITSARA M, CHINTHURAN T, FATEMEH A, et al. 26355 Alopecia areata:A rare side effect of prazosin[J]. Journal of the American Academy of Dermatology,2021,85(3S):98.
[10]	国家食品药品监督管理局. BJS 2008016补肾壮阳类中成药中西地那非及其类似物的检测[S]. 北京:中国标准出版社, 2008. [National Food and Medical Products Administration. BJS 2008016 Detection of sildenafil and analogues in Chinese patent medicines for invigorating kidney-yang[S]. Beijing:Standards Press of China, 2008.] National Food and Medical Products Administration. BJS 2008016 Detection of sildenafil and analogues in Chinese patent medicines for invigorating kidney-yang[S]. Beijing: Standards Press of China, 2008.
[11]	国家食品药品监督管理局. BJS 2009030补肾壮阳类中成药中PDE5型抑制剂的快速检测方法[S]. 北京:中国标准出版社, 2009. [National Food and Medical Products Administration. BJS 2009030 Rapid detection method of PDE5 inhibitors in Chinese patent medicines for invigorating kidney-yang[S]. Beijing:Standards Press of China, 2009.] National Food and Medical Products Administration. BJS 2009030 Rapid detection method of PDE5 inhibitors in Chinese patent medicines for invigorating kidney-yang[S]. Beijing: Standards Press of China, 2009.
[12]	国家食品药品监督管理局. BJS 201710保健食品中75种非法添加化学药物的检测[S]. 北京:中国标准出版社, 2017. [National Food and Medical Products Administration. BJS 201710 Detection of 75 illegally added chemical drugs in health food [S]. Beijing:Standards Press of China, 2017.] National Food and Medical Products Administration. BJS 201710 Detection of 75 illegally added chemical drugs in health food [S]. Beijing: Standards Press of China, 2017.
[13]	国家食品药品监督管理局. BJS 201805食品中那非物质的测定[S]. 北京:中国标准出版社, 2018. [National Food and Medical Products Administration. BJS 201805 Determination of nafils substances in foods[S]. Beijing:Standards Press of China, 2018.] National Food and Medical Products Administration. BJS 201805 Determination of nafils substances in foods[S]. Beijing: Standards Press of China, 2018.
[14]	中华人民共和国海关总署. SN/T 5357-2021 出口保健食品中多种非法添加物的测定液相色谱-质谱/质谱法[S]. 北京:中国标准出版社, 2021. [General Administration of Customs of the People's Republic of China. SN/T 5357-2021 Determination of multi-groups of illegal additives in health foods for export-LC-MS/MS method[S]. Beijing:Standards Press of China, 2018.] General Administration of Customs of the People's Republic of China. SN/T 5357-2021 Determination of multi-groups of illegal additives in health foods for export-LC-MS/MS method[S]. Beijing: Standards Press of China, 2018.
[15]	VIEIRALVES R R, FAVORITO L A. Dapoxetine and premature ejaculation[J]. International Brazilian Journal of Urology,2023,49(4):511−514. doi: 10.1590/s1677-5538.ibju.2023.9908
[16]	刘竹芳, 黄文柱, 刘敏, 等. 5α还原酶抑制剂联用α受体阻断剂对老年良性前列腺增生下尿路症状的效果及护理要点[J]. 中国药业,2017,26(12):38−40. [LIU Z F, HUANG W Z, LIU M, et al. Clinical effect of 5α reductase inhibitors combined with α receptor blockers in treating lower urinary tract symptoms in elderly patients with benign prostatic hyperplasia and its main nursing points[J]. China Pharmaceuticals,2017,26(12):38−40.] doi: 10.3969/j.issn.1006-4931.2017.12.010 LIU Z F, HUANG W Z, LIU M, et al. Clinical effect of 5α reductase inhibitors combined with α receptor blockers in treating lower urinary tract symptoms in elderly patients with benign prostatic hyperplasia and its main nursing points[J]. China Pharmaceuticals, 2017, 26（12）: 38−40. doi: 10.3969/j.issn.1006-4931.2017.12.010
[17]	MANDOUR A A, ELKAEED E B, HAGRAS M, et al. Virtual screening approach for the discovery of selective 5α-reductase type II inhibitors for benign prostatic hyperplasia treatment[J]. Future Medicinal Chemistry, 2023, 15(23): 2149−2163.
[18]	WANG X, ZHAO J J, ZHANG Q, et al. A chemometric strategy for accurately identifying illegal additive compounds in health foods by using ultra-high-performance liquid chromatography coupled to high resolution mass spectrometry[J]. Analytical Methods:Advancing Methods and Applications,2021,13(14):1731−1739.
[19]	邵瑞婷, 丁学妍, 姜洁. 超高效液相色谱-串联质谱法测定调节三高类保健食品中59种非法添加药物[J]. 食品科学,2024,45(9):232−242. [SHAO R T, DING X Y, JIANG J. Determination of 59 illegal addition of drugs in three kinds of regulated health food by ultrahigh performance liquid chromatography-tandem mass spectrometry[J]. Food Science,2024,45(9):232−242.] doi: 10.7506/spkx1002-6630-20230628-221 SHAO R T, DING X Y, JIANG J. Determination of 59 illegal addition of drugs in three kinds of regulated health food by ultrahigh performance liquid chromatography-tandem mass spectrometry[J]. Food Science, 2024, 45（9）: 232−242. doi: 10.7506/spkx1002-6630-20230628-221
[20]	XIE X J, ZHANG Y, YUE Z F, et al. Multi-fingerprint profiling analysis for screening and quantification of illegal adulterated antidiabetics in a functional food using HPLC coupled to diode array detection/fluorescence detection[J]. Microchemical Journal,2019,149:103995. doi: 10.1016/j.microc.2019.103995
[21]	胡燕. 高效液相色谱法检测茶叶中5种非法添加合成着色剂[J]. 食品与发酵科技,2023,59(4):137−142. [HU Y. Determination of five synthetic colorants illegally added in teasamples by high performance liquid chromatography[J]. Food and Fermentation Science & Technology,2023,59(4):137−142.] HU Y. Determination of five synthetic colorants illegally added in teasamples by high performance liquid chromatography[J]. Food and Fermentation Science & Technology, 2023, 59（4）: 137−142.
[22]	XU D M, LAI G Y, CHEN Y, et al. Simultaneous determination of 21 illegally added compounds in health foods by ultra high performance liquid chromatography-tandem mass spectrometry with solid phase extraction[J]. Chinese Journal of Chromatography,2019,37(7):778−785. doi: 10.3724/SP.J.1123.2019.01016
[23]	WU Z Q, JIANG X Q, YANG Y Q, et al. Amphiphilic polymers facilitated solid phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry for direct extraction and analysis of zearalenone and zearalanone in corn juice samples[J]. Journal of Separation Science,2023,46(14):e2300112. doi: 10.1002/jssc.202300112
[24]	PENG S, CHAO W, LEI X, et al. Simultaneous determination of eight phenolic acids in rapeseed by accelerated solvent extraction-solid phase extraction ultra-high-performance liquid chromatography-tandem mass spectrometry[J]. Food Analytical Methods,2022,15(10):2625−2632. doi: 10.1007/s12161-022-02310-6
[25]	HASSAN Y A A, AYAD M F, HUSSEIN L A, et al. Hydrophilic interaction liquid chromatography (HILIC) with DAD detection for the determination of relatively non polar fungicides in orange samples[J]. Microchemical Journal,2023,193:109196. doi: 10.1016/j.microc.2023.109196
[26]	SOUMIA B E, DOMENICA M, KATIA A, et al. Determination of astaxanthin and astaxanthin esters in three samples of shrimp waste (Parapenaeus longirostris) by high performance liquid chromatography coupled photo-diode array and mass spectrometry detection[J]. Natural Product Research,2023,193:1−8.
[27]	SUMIA A, BUSHRA S, RAFIQUE M A, et al. Salting-out assisted liquid-liquid microextraction and reverse-phase chromatographic quantification of two neonicotinoid insecticides from fruits and vegetables[J]. Journal of Chromatographic Science,2023,61(9):875−884. doi: 10.1093/chromsci/bmad055
[28]	宁霄, 金绍明, 李志远, 等. 功能性老年乳粉中300种非法添加药物及其类似物的液相色谱-高分辨质谱分析[J]. 色谱,2023,41(11):960−975. [NING X, JIN S M, LI Z Y, et al. Liquid chromatography-high resolution mass spectrometry analysis of 300 illegally added drugs and their analogues in functional milk powder for the elderly[J]. Chinese Journal of Chromatography,2023,41(11):960−975.] doi: 10.3724/SP.J.1123.2023.05013 NING X, JIN S M, LI Z Y, et al. Liquid chromatography-high resolution mass spectrometry analysis of 300 illegally added drugs and their analogues in functional milk powder for the elderly[J]. Chinese Journal of Chromatography, 2023, 41（11）: 960−975. doi: 10.3724/SP.J.1123.2023.05013
[29]	周建峰, 王晓园, 隋玉杰, 等. QuEChERS-超高效液相色谱-串联质谱法快速筛查食疗膳食中40种非法添加药物[J]. 分析科学学报,2023,39(4):461−468. [ZHOU J F, WANG X Y, SUI Y J, et al. Determination of 40 kinds of illegally additives in medicine dietary by QuEChERS-ultra-performance liquid chromatography-tandem mass spectrometry[J]. Journal of Analytical Science,2023,39(4):461−468.] ZHOU J F, WANG X Y, SUI Y J, et al. Determination of 40 kinds of illegally additives in medicine dietary by QuEChERS-ultra-performance liquid chromatography-tandem mass spectrometry[J]. Journal of Analytical Science, 2023, 39（4）: 461−468.
[30]	XU H B, ZHANG S P, DU R Y, et al. Ultra-high performance liquid chromatography-orbitrap high-resolution mass spectrometry for rapid screening and identification of 32 illegally added drugs in slimming and anti-impotence health foods[J]. Chinese Journal of Chromatography,2022,40(6):531−540. doi: 10.3724/SP.J.1123.2021.12009
[31]	李莲微, 杨晓聪, 姚闽娜, 等. 超高效液相色谱-串联质谱法同时测定动物源性食品中9种多肽类抗生素残留[J]. 食品工业科技,2022,43(17):330−337. [LI L W, YANG X C, YAO M N, et al. Determination of nine polypeptide antibiotics residues in animal tissue food by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry,2022,43(17):330−337.] LI L W, YANG X C, YAO M N, et al. Determination of nine polypeptide antibiotics residues in animal tissue food by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry, 2022, 43（17）: 330−337.
[32]	VARSHA R, JASHBIR S, JAYAPRAKASHA G K, et al. Op-timization of extraction solvent and fast blue BB assay for comparative analysis of antioxidant phenolics from Cucumis melo L.[J]. Plants,2021,10(7):1379. doi: 10.3390/plants10071379
[33]	袁利杰, 赵光升, 张培毅, 等. UPLC-MS/MS测定小麦粉及其制品与小麦粉添加剂中新型非法添加物乙酰氧肟酸[J]. 现代食品科技,2024,40(1):281−287. [YUAN L J, ZHAO G S, ZHANG P Y, et al. Determination of new illegal additive acetohydroxamic acid in wheat flour and its products and wheat flour additive by UPLC-MS/MS[J]. Modern Food Science and Technology,2024,40(1):281−287.] YUAN L J, ZHAO G S, ZHANG P Y, et al. Determination of new illegal additive acetohydroxamic acid in wheat flour and its products and wheat flour additive by UPLC-MS/MS[J]. Modern Food Science and Technology, 2024, 40（1）: 281−287.
[34]	寻坚兰. 琥珀酸噁泼西汀高效液相色谱分析方法开发[D]. 上海:上海交通大学, 2020:29-30. [XUN J L. Developing a method for the determination of esreboxetine succinate by high performance liquid chromatography[D]. Shanghai:Shanghai Jiao Tong University, 2020:29-30.] XUN J L. Developing a method for the determination of esreboxetine succinate by high performance liquid chromatography[D]. Shanghai: Shanghai Jiao Tong University, 2020: 29-30.
[35]	HINAL H, KASHYAP T, SANJAY C, et al. HPTLC method development and validation for the simultaneous estimation of nortriptyline HCl and pregabalin in their combined dosage form[J]. Journal of Chromatographic Science, 2023.
[36]	FU X X, ZHONG L L, WANG R, et al. Exploration of a high-performance liquid chromatography method for antibiotic drug monitoring in melioidosis patients and establishment of its sampling procedure[J]. Results in Chemistry,2023,6:101187. doi: 10.1016/j.rechem.2023.101187

Supplements (1)

Supplements
Other Related Supplements

Get Citation

PDF

XML

Article Metrics

Article views (65) PDF downloads (15)

Science and Technology of Food Industry

Simultaneously Detecting of 13 Illegally Added Drugs in Health Foods for Improving Sexual and Prostate Function by High Performance Liquid Chromatography

Abstract

References

Supplements

Other Related Supplements

Catalog

Article Metrics

Export File

Citation

Format

Content