Optimization of preparation process of Enteromorpha prolifera polysaccharide-iron and its structure characterization

The optimal chelating conditions for Enteromorpha prolifera polysaccharide-iron were investigated by response surface methodology(RSM). It was characterized through fourier transform infrared(FTIR)spectroscopy and X-ray diffraction. Results showed that the optimal reaction conditions were temperature 54 ℃,chelating time 3.8 h,the mass ration of polysaccharide and ferric iron 3.9∶1,and pH7.5. The chelating rate could reach 19.6%. The FTIR indicated that the hydroxyl and carboxyl groups were chelated with Fe³⁺. The generation of E. prolifera polysaccharide-iron was further confirmed by X-ray diffraction to provide scientific basis for the high-value utilization of E. prolifera resource and the exploitation of a new iron supplement.