黑米花青苷复方胶囊对酒精性肝损伤的防治作用
详细信息Preventive and curative effect of black rice anthocyanin compound capsule on alcohol-induced liver injury
-
摘要: 目的:研究黑米花青苷复方胶囊在小鼠酒精性肝损伤中的防治作用。方法:建立小鼠酒精性肝损伤病理模型,分别灌胃低剂量、高剂量的黑米花青苷复方胶囊进行治疗,正常组灌胃蒸馏水,助悬剂组灌胃植物油。第12d,测定肝组织中超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量、谷胱甘肽（GSH）含量及肿瘤坏死因子-α（TNF-α）和白介素-lβ（IL-lβ）的水平,并进行肝脏病理学观察。结果:黑米花青苷复方胶囊能明显提高肝组织中SOD的活力,对MDA起到直接清除作用;还可以提高GSH含量,降低TNF-α和IL-lβ的水平。病理组织学显示,黑米花青苷胶囊能显著改善肝细胞的脂肪变性。同时,助悬剂对药物的效果没有影响。结论:黑米花青苷复方胶囊能够对酒精性肝损伤的治疗起到一定的效果。Abstract: Established pathology model of alcoholic-induced liver injury in mice and cured by poured the black rice anthocyanin compound capsule in low concentration and high concentration into stomach, distilled water for the normal group and plant oil for the suspending agents group. The content of SOD, MDA, GSH and the levels of TNF-α&IL-lβ in liver homogenate were measured in the 12th day. Hepatic pathological examination was observed. The result showed that the black rice anthocyanin compound capsule could increase SOD activity obviously, clear MDA content directly in hepatic tissue, improve GSH content and reduce TNF-α&IL-lβ level and improve hepatic steatosis. At the same time, suspension agents basically had no influence on drug effect. Therefore the black rice anthocyanin compound capsule had a particular treatment effect on alcohol liver injury in mice.
-
[1] 孔令瑶.黑米中生物活性物质的研究[D].无锡:江南大学, 2008. [2] 曹小勇, 李新生.黑米花色素苷类色素研究现状及展望[J].氨基酸和生物资源, 2002, 24 (1) :3-6. [3] 王锋, 邓洁, 谭兴和, 等.花色苷及其共色作用研究进展[J].食品科学, 2008, 29 (2) :472-476. [4] Seok H N, Sun P C, Mi Y K, et al.Antioxidative activities of bran extracts from twenty one pigmented rice cultivars[J].Food Chemistry, 2006, 94:613-620.
[5] 王艳龙, 石绍福, 韩豪, 等.中国黑米花色苷研究现状及展望[J].中国生化药物杂志, 2010, 31 (1) :63-66. [6] 李春阳, 许时婴, 王璋.葡萄籽原花青素结构单元的红外光谱分析[J].食品与生物技术学报, 2005, 24 (4) :47-51. [7] Li xingsheng, Long shuming, Wu sanqiao, et al.Investigation of anthocyanin extraction from black rice and light stability of anthocyanin-containing alcoholic drink[J].CIGR Journal, 2011, 13 (Special issue) :151-159
[8] 吴三桥, 史隋孝, 丁锐, 等.黑米中花青苷色素的测定方法研究[J].氨基酸和生物资源, 2002, 24 (3) :66-68. [9] 胡秋林.黑米色素药理动物实验研究报告[J].武汉食品工业学院学报, 1997, 12 (3) :10-12. [10] 秦玉, 凌文华.黑米花色苷提取物胶囊对高血脂症病人的降血脂作用[J].食品科学, 2008, 29 (10) :540-542. [11] 杨靖亚, 吴宏忠, 胡怿, 等.天然抗氧化剂-抗癌转移的新途径[J].中国临床药理学与治疗学, 2007, 12 (4) :366-370. [12] Zhang Ming wei, Guo Bao jiang, Chi Jian wei, et al.Antioxidations and their correlation with total flavones and anthocyanin contents in different black rice varieties[J].Agric Sci China, 2005, 38 (7) :1324-1334.
[13] 石绍福.黑米花青苷组分分析及软胶囊研制[D].汉中:陕西理工学院, 2011. [14] 李业钊, 李航.乙醇代谢酶与乙醇性肝损伤[J].医学综述, 2008, 14 (18) :2788-2791. [15] Horie Y, Kato S, Ohki E, et al.Hepatic micro vascular dysfunctionin endotoxemie rats after acute ethanol administration[J].AlcoholClin Exp Re, 2000, 24 (5) :691-698.
[16] Kishore R, Hill J R, McMullen M R, et al.ERK1/2and Egr-1contribute to increased TNF-alpha production in rat Kupffer cellsafter chronic ethanol feting[J].Am J Physiol Gastrointest LiverPhysiol, 2002, 282 (1) :6-15.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
